Research Journal of Chemical Sciences ______________________________________________ ISSN 2231-606X Vol. 2(7), 62-68, July (2012) Res.J.Chem. Sci. International Science Congress Association 62 Synthesis and Antimicrobial Activity of Azetidin-2-one Containing Pyrazoline DerivativesShah Shailesh H.1* and Patel Pankaj S.Dept. of Chem., Patel JBR Arts, Patel AMR Commerce and Patel JDKD Science College, Borsad, GJ, & JJT-University, RJ, INDIA Department of Chemistry, Sheth LH Science College, Mansa, Gujarat, INDIA Available online at: www.isca.in Received 11th May 2012, revised 22th May 2012, accepted 24th May 2012Abstract Pyrazolines are well-known and important nitrogen containing 5-membered heterocyclic compounds and various methods have been worked out for their synthesis. A new series of 3-chloro-1-{4-[5-(Substitutedphenyl)-4,5-dihydro-pyrazol-3-yl]phenyl}-4-(4-hydroxyphenyl) azetidin-2-one are synthesized by reacting 3-chloro-1-{4-[3-(Substituted phenyl)prop-2-enoyl]phenyl}-4-(4-hydroxyphenyl)azetidin-2-one with 99% hydrazine hydrate. All these compounds were characterized by means of their IR, H NMR, Spectroscopic data and microanalysis. All the synthesized products were evaluated for their antimicrobial activity. All the compounds were tested for their antibacterial and antifungal activities by broth dilution method. Keywords: Chalcones, 2-pyrazolines, azetidin-2-one, antimicrobial activity. Introduction Pyrazol belongs to the family of azoles, five membered heterocycles; pyrazolines have proved to be the most useful framework for biological activities. Pyrazolines have attracted attention of medicinal chemists for both with regard to heterocyclic chemistry and the pharmacological activities associated with them. The pharmaceutical importance of these compounds lies in the fact that they can be effectively utilized as antibacterial, antifungal, antiviral, antiparasitic, antitubercular and insecticidal agents. As evident from the literature, in recent years a significant portion of research work in heterocyclic chemistry has been devoted to pyrazolines containing different aryl groups as substituents. 2-Azetidinones, commonly known as beta-lactams, are well-known heterocyclic compounds among the organic and medicinal chemists .The activity of the famous antibiotics such as penicillin, cephalosporin, monobactams and carbapenems are attributed to the presence of 2-azetidinone ring in them. Azetidin-2-ones can be prepared from Schiff’s bases, which are the condensation products of aldehydes and amino compounds. They are considered significant owing to their wide range of biological application. Recently, some other types of biological activity besides the antibacterial activity have been reported in compounds containing 2-azetidinone ring. Such biological activities include antimicrobial, anti-tubercular, carbonic anhydrase inhibitors, local anesthetics, anti-inflammatory, anthelmintic, anticonvulsant, hypoglycemic activity2-4. 2-pyrazolines are reported as antibacterial, antifungal6-8, antimicrobial, antiviral10, anti-arthritis11 and anti-inflammatory12 agents. Encouraged by these facts, we selected to work on Azetidin-2-one containing Pyrazoline with different substitutions on the phenyl ring. In the present study we report the reaction of 3-chloro-1-{4-[3-(substituted phenyl) prop-2-enoyl] phenyl} - 4-(4-hydroxyphenyl) azetidin-2-one with 99% hydrazine hydrate to form pyrazoline (4a-j). The structures of the various synthesized compounds were assigned on the basis of IR, H-NMR spectral data and elemental analysis (table-1). These compounds were also screened for their antimicrobial activity. Material and Methods The IR spectra were recorded on IR affinity-1, DRS-8000A, Shimadzu, Ptc. Ltd., Japan spectrophotometer. The H-NMR was recorded in DMSO on Bruker Advance II 400 MHz spectrometer using TMS as an internal standard. Melting points were determined in open capillary tubes and are uncorrected. The purity of the compounds was checked by TLC-using Silica gel-G (Merck). Column chromatography was performed on silica gel. All the compounds were tested for their antibacterial and antifungal activities by broth dilution method.Synthesis of 1-(4-{[(4-hydroxyphenyl) methylene] amino} phenyl) ethanone (1) : A mixture of 4-hydroxy benzaldehyde (0.01M), 1-(4-aminophenyl) ethanone (0.01M) and methanol (30ml) was heated for about 5 min. in a beaker (250 ml) to get a clear solution. The solution was kept overnight at room temperature to get the respective crude solid which was recrystallized from ethanol to obtain the pure crystals of 1-(4-{[(4-hydroxy phenyl)methylene]amino}phenyl) ethanone respectively. The yield of the product was 75% and the product melts at 195C. Found: C(75.28%) H(5.45%) N(5.82%) , Calcd. Research Journal of Chemical Sciences ___________________________________________________________ ISSN 2231-606XVol. 2(7), 62-68, July (2012) Res. J. Chem. Sci. International Science Congress Association 63 for C1513NO: C(75.30%) H(5.48%) N(5.85%). IR, cm-1:3085 (-OH), 3040 (=C-H), 2920(-C-H), 1676(�C=O), 1647(�C=N-), 1606 (�C=C), 1363(-CH, bend), 1314(-C-N), 1284 (-C-O-), 1240(-C-CO-C-). H-NMR (DMSO, , ppm): 2.5692 (3H, s, COCH), 6.5277-7.9774 (8H, m, Ar-H), 8.3820 (1H, s, -CH=N-), 9.6392 ( 1H, s, Ar-OH). Synthesis of 1-(4-acetylphenyl)-3-chloro-4-(4-hydroxyphenyl) azetidin -2-one (2): In a 100ml Round bottom flask 1-(4-{[(4-hydroxyphenyl) methylene] amino} phenyl) ethanone (0.01M) in 70ml benzene was taken. Chloro acetyl chloride (0.01M) was added at room temperature with constant stirring and triethylamine 1ml was added and the reaction mixture was refluxed for 7 hours. After the completion of reaction, solvent was removed by vacuum distillation. The solid was filtered, dried and recrystallized from toluene. The yield of the product was 60% and the product melts at 119C. Found: C(64.64%) H(4.44%) N(4.42%), Calcd. for C1714ClNO: C(64.67%) H(4.47%) N(4.44%). IR, cm-1: 3300 (-OH), 3050(=C-H), 2950(-C-H), 1680(&#x-3.3;å ¦C=O), 1600(&#x-3.3;å ¦C=C), 1375(-CH, bend), 1300(-C-N), 1240(-C-CO-C-), 1220(-C-O), 560 (C-Cl). H-NMR (DMSO, , ppm): 2.5392 (3H, s, COCH), 4.8954 (1H, d, &#x-3.3;å ¦CH-Ar), 5.5151 (1H, d, &#x-3.3;å ¦CH-Cl), 6.6720-8.0745 (8H, m, Ar-H), 9.7784 ( 1H, s, Ar-OH).Synthesis of 3-chloro-1-{4-[3-(Substituted phenyl) prop-2-enoyl] phenyl}-4-(4-hydroxyphenyl) azetidin-2-one (3a-j): To the solution of 1-(4-acetylphenyl)-3-chloro-4-(4-hydroxyphenyl) azetidin-2-one (0.01M) in absolute ethanol (50 ml), substituted benzaldehyde (0.01M) and 2% NaOH were added and refluxed for 10 hours. After refluxing the reaction mixture was concentrated, cooled, filtered and neutralized with dil. HCl. The solid residue thus obtained was crystallized by absolute ethanol. IR(3b), cm-1:3359(-OH), 3045(=C-H), 1728(&#x-3.3;å ¦C=O), 1608(&#x-3.3;å ¦C=C), 1290(-C-N), 1186 (-C-O-), 769(-C-Cl).H-NMR (3c-DMSO, , ppm): 3.8789 (6H, s, -OCH), 4.8613 (1H, d, &#x-3.3;女CH-Ar), 5.3413 (1H, d, &#x-3.3;女CH-Cl), 6.7340-7.8883 (11H, m, Ar-H), 7.9733 (2H, d, -CH=CH-), 9.8306 ( 1H, s, Ar-OH). Synthesis of 3-chloro-1-{4-[5-(Substituted phenyl)-4,5-dihydro-pyrazol-3-yl]phenyl}-4-(4-hydroxyphenyl) azetidin-2-one.(4a-j): A mixture of 3-chloro-1-{4-[3-(Substituted phenyl) prop-2-enoyl] phenyl}-4-(4-hydroxyphenyl) azetidin-2-one (0.01M) and 99% hydrazine hydrate (0.015M) in ethanol (50ml) refluxed gently for 3 hours. Then the mixture was concentrated and allowed to cool. The resulting solid was filtered, washed with ethanol and recrystallized from ethanol to give a pale brown solid. IR(4f), cm-1: 3317 (-OH), 3080 (=C-H), 1718(&#x-3.3;女C=O), 1658(&#x-3.3;女C=N-), 1544 (&#x-3.3;女C=C), 1460(-CH, bend), 1324(-C-N),1284 (-N-N), 1234 (-C-O),641 (-C-Cl-), 3463 (&#x-3.3;女NH). H-NMR (4h-DMSO, , ppm): 3.1699 (6H, s, N(CH), 3.9462 (2H, d, CH- of Pyrazol), 4.3000 (1H, t, &#x-3.3;女CH-Ar of Pyrazol), 4.8268 (1H, d, &#x-3.3;女CH-Ar of Azetidine), 5.3981 (1H, d, &#x-3.3;女CH-Cl of Azetidine), 6.6114-7.9986 (13H, m, Ar-H, -NH-), 9.5428 ( 1H, s, Ar-OH). Table-1 Physical constant of 3-chloro-1-{4-[5-(Substituted phenyl)-4, 5-dihydro-pyrazol-3- yl] phenyl}-4-(4-hydroxyphenyl) azetidin-2-one Comp R Molecular formula Yield % M.P. °°C Elemental Analysis % C Found (Calcd) % N Found (Calcd) % H Found (Calcd) 4a -2-Cl C2419Cl 68 173 63.70 (63.73) 9.26 (9.29) 4.19 (4.23) 4b -2-OH C2420ClN 70 182 66.42 (66.44) 9.62 (9.68) 4.61 (4.65) 4c -3,4-(OCH 3 ) 2 2624ClN 65 135 65.28 (65.34) 8.76 (8.79) 5.03 (5.06) 4d -3-NO C2419ClN 74 188 62.24 (62.27) 12.08 (12.10) 4.11 (4.14) 4e -4-Cl C2419Cl 68 278 63.72 (63.73) 9.27 (9.29) 4.19 (4.23) 4f -4-N(C 2 H 5 ) 2 2829ClN 64 138 68.74 (68.77) 11.43 (11.46) 5.94 (5.98) 4g -4-OH C2420ClN 72 178 66.40 (66.44) 9.63 (9.68) 4.60 (4.65) 4h -4-N(CH C2625ClN 63 162 67.73 (67.75) 12.12 (12.15) 5.44 (5.47) 4i CHO C2420ClN2 72 203 68.95 (68.98) 10.02 (10.06) 4.79 (4.82) 4j -2-OH- 3-OCH 3 2522ClN4 73 208 64.69 (64.72) 9.03 (9.06) 4.75 (4.78) Research Journal of Chemical Sciences ___________________________________________________________ ISSN 2231-606XVol. 2(7), 62-68, July (2012) Res. J. Chem. Sci. International Science Congress Association 64 Results and Discussions Antimicrobial activity: The MICs of synthesized compounds were carried out by broth micro dilution method as described by Rattan (2000). It is one of the non automated in vitro bacterial susceptibility tests. This classic method yields a quantitative result for the amount of antimicrobial agents that is needed to inhibit growth of specific microorganisms. The in vitro antimicrobial activity of test compounds were assessed against 24 hr cultures of several selected bacteria and fungi. The bacteria used were E. coli, S.aureus, P. aeruginosa, and S. pyogenus; the fungi used were C. albicans, A. Niger, and A.clavatus. The antimicrobial activity was performed by broth dilution method in DMSO. Gentamycin, ampicilin, chloramphenicol, ciprofloxacin, norfloxacin, nystatin and greseofulvin were used as standard for the evaluation of antibacterial and antifungal activities respectively. The activity was reported by minimal inhibition concentration. The results are summarized in table-2 Table-2 Antimicrobial activities 3-chloro-1-{4-[5-(Substituted phenyl)-4, 5-dihydro-pyrazol-3-yl] phenyl}-4-(4-hydroxyphenyl) azetidin-2-one Compound R Antibacterial activity Minimal Inhibition Concentration Antifungal Activity Minimal Inhibition Concentration E.COLI P.AER UGINOSA S.AUREUS S.PYOGENUS C.ALB ICANS A.NIGER A.CLA VATUS 4a -2-Cl 100 150 125 100 1000 1000 800 4b -2-OH 175 200 175 250 800 700 700 4c -3,4-(OCH 3 ) 2 225 225 150 200 �1000 800 600 4d -3-NO 2 175 225 200 150 700 600 1000 4e -4-Cl 100 175 100 175 700 �1000 1000 4f -4-N(C 2 H 5 ) 2 250 200 1250 200 500 1000 1000 4g -4-OH 200 175 200 175 800 800 800 4h -4-N(CH 3 ) 2 200 200 175 225 700 700 700 4i CHO 175 225 200 125 750 600 800 4j -2-OH- 3-OCH 3 200 200 225 200 1000 800 �1000 Table-3 Antibacterial Activity: Minimal Inhibition Concentration (The Standard Drugs) Drug E.Coli P. Aeruginosa S. Aureus S. Pyogenus - MTCC 443 MTCC 1688 MTCC 96 MTCC 442 (Microgramme/ml) Gentamycin 0.05 1 0.25 0.5 Ampicillin 100 -- 250 100 Chloramphenicol 50 50 50 50 Ciprofloxacin 25 25 50 50 Norfloxacin 10 10 10 10 Table-4 Antifungal Activity: Minimal Inhibition Concentration (The Standard Drugs) DRUG C.ALBICANS A.NIGER A.CLAVATUS - MTCC 227 MTCC 282 MTCC 1323 (Microgramme/ml) Nystatin 100 100 100 Greseofulvin 500 100 100 Research Journal of Chemical Sciences ___________________________________________________________ ISSN 2231-606XVol. 2(7), 62-68, July (2012) Res. J. Chem. Sci. International Science Congress Association 65 Biological screening result of activities 3-chloro-1-{4-[5-(substituted phenyl)-4, 5-dihydro-pyrazol-3-yl] phenyl}-4-(4-hydroxyphenyl) azetidin-2-one based derivatives shows that compound (4a) have shown better activity against E. coli and S. pyogenus, while (4e) have Shawn better activity against E.coli, while rest of all compound possessed good activity against S.aureus in the range of 100-225 g/ml. Compounds with substitution 2-Chloro (4a), shown good antibacterial activity against S. pyogenus , while rest of all derivatives possessed good activity against S. pyogenus in the range of 125-250 g/ml. Compound (4f) is found to be good antifungal activity against C. albicans, against standard drugs Greseofulvin. While rest of all derivatives are poor against A. Niger and A. clavatus Conclusion The Main focus of this research work was to synthesize, characterize and evaluate antimicrobial activities of the newly synthesized pyrazoline derivatives, structures of synthesized compounds were confirmed and characterized with the help of analytical data’s such as IR and H-NMR. In summary, we have described the synthesis and antimicrobial activity of some new 3-chloro-1-{4-[5-(substituted phenyl)-4, 5-dihydro-pyrazol-3-yl] phenyl}-4-(4-hydroxyphenyl) azetidin-2-one MIC values revealed that amongst newly synthesized compound having chlorophenyl type linkage has shown good activity against the bacterial strains..AcknowledgementThe authors are thankful to the Principal and Management of Arts, Commerce and Science College, Borsad and Mansa for providing laboratory facilities, SAIF, Chandigarh for NMR Spectra and Loyola Research Center- Xavier’s College, Ahmedabad for IR spectra and micro-care laboratory, Surat, Gujarat, India for biological activity. 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Pyrazoline Bisphosphonate esters as novel anti-inflammatory and antiarthritic agents, J Med Chem., 36, 134–8,(1993)Figure-1 IR Spectra of (1) Research Journal of Chemical Sciences ___________________________________________________________ ISSN 2231-606XVol. 2(7), 62-68, July (2012) Res. J. Chem. Sci. International Science Congress Association 66 OOH methanolCH ClCOCHClEtOHClCH EthanolEt-OH4a-jReaction Scheme O R ClOH R NHClOH NHNH CH 3a-jScheme Figure-2 IR Spectra of (2) Research Journal of Chemical Sciences ___________________________________________________________ ISSN 2231-606XVol. 2(7), 62-68, July (2012) Res. J. Chem. Sci. International Science Congress Association 67 Figure-3 IR Spectra of 3b Figure-4 IR Spectra of 4f Figure-5 NMR Spectra of (1) Research Journal of Chemical Sciences ___________________________________________________________ ISSN 2231-606XVol. 2(7), 62-68, July (2012) Res. J. Chem. Sci. International Science Congress Association 68 Figure-6 NMR Spectra of (2) Figure-7 NMR Spectra of 3c Figure-8 NMR Spectra of 4h