Research Journal of Chemical Sciences ________________________________________ ISSN 2231-606X Vol. 1(5), 85-87, Aug. (2011) Res.J.Chem.Sci. International Science Congress Association 85 Short CommunicationSynthesis and Biological activity for 4-Methoxyphenyl (pyrazin-2-yl) Methanone and its detection by GC-MS Techniques Dalvi S.N. and Garge S.S.2 Dept. of Physics, SN Arts, DJM Commerce and BNS Science College, Sangamner, Ahmednagar, Pin– 422605 INDIA PDF Applied research and Development Institute, Ahmednagar, Maharashtra INDIA Available online at: www.isca.in (Received 30th June 2011, revised 3rd July 2011, accepted 23rd July 2011)Abstract The synthesis of 4-methoxyphenyl (pyrazin-2-yl) methanone and its detection by GC-MS technique by p-methoxybenzaldehyde reacted with pyrazin-2-yl in the presence of suitable solvent in different conditionto form p-methoxybenzaldehyde. The structure detected with GC-MS and the compound identified with T.L.C. technique and then examined for antibacterial activity. Key words:4-methoxyphenyl (pyrazin-2-yl), GC-MS, antibacterial activity, p-methoxybenzaldehyde derivatives. IntroductionThe microorganisms can produce the phenol derivatives and may find in insects, have various biological activities such as anti-microbial and anti-tumor4 activities1,2,3,4,5,6. Although biological activity of actinomycin elucidated, other phenoxyazine derivatives have not been examined well. The literature search reveals that the Schiff base derived from 4-methoxyphenyl (pyrazin-2-yl) methanone l has not been reported. The nitrogen of the quinoxaline unit, an acceptor for hydrogen bonding and may lead to polymeric structures. The electronic environment in the metal complexes of this Schiff base from those derived from salicylaldehyde might different. In this article, we describe the studied various novel p-methoxybenzaldehyde derivatives 4-methoxyphenyl (pyrazin-2-yl) methanone were preparation and detected by GC-MS and the antibacterial activity of derivatives. Material and MethodsPreparation of 4-methoxyphenyl (pyrazin-2-yl) methanone: The2.7 gm of p-methoxybenzaldehyde dissolved in 20 ml solvent and the 1.4 gm of pyrazin-2-yl dissolved in 20 ml solvent and then add 0.5 gm sodium acetate and let it be dissolved completely. The p-methoxybenzaldehyde mixture is added drop by drop with continuous stirring. This mixture is reflux for one hour at 90C temperature with continuous stirring. After an hour, stop heating and cool the mixture at room temp and now it’s ready for further analysis. The mixture filtration is done with separation of the solvent by simple distillation methods. Study of Antibacterial Activity: In the present research work the analysis done for the antibacterial activity spectrum of p-methoxybenzaldehyde derivates. The Gram-positive bacteria, Staphylococcusaureusand Gram-negative bacteria Escherichiacoli are used for this study. The inoculums size adjusted to 1 to 2 × 107 CFU (colony forming units)/ml by serial dilution with sterilized nutrient broth media. The nutrient agar (pH 7.2-7.4) used for routine susceptibility and testing of nonfastidious bacteria. The stock solution 10000 g/ml is prepared in 20% v/v water in DMSO. By using the stock solution, 6000 g/ml, 4000 g/ml, 2000 g/ml and 1500 g/ml solution preparation done and out of which 100 l solution taken for assay. The Ciprofloxacin used as a standard. The 20% v/v WFI in DMSO used as a control. The antibacterial assay carried out by agar well diffusion method9,10. After 16 to 18 hours period of incubation, examination of each plate was done. Results and Discussion The results of preliminary evaluation showed that the antibacterial activity of the derivatives from p-methoxybenzaldehyde evaluated at two different concentrations by the diffusion method. The derivatives from p-methoxybenzaldehyde show the antibacterial activity at varied levels inthe E. coli and S. aureus. The S. Aureus was found less active in inhibition zone. The antibacterial activity of derivatives from p-methoxybenzaldehyde has seen and its detection with suitable solvent in different condition p-methoxybenzaldehyde which reacted with pyrazin-2-yl to form p-methoxybenzaldehyde done by GC-MS. The structures detected with GC-MS and compounds checked with TLC technique and detection for antibacterial activity. ConclusionIn presence of p-methoxybenzaldehyde, synthesis of 4-methoxyphenyl (pyrazin-2-yl) methanone of 3-(chloromethyl) -9-ethyl-9H-carbazole done with suitable solvent in different conditions and the reaction also proceeds in different media without using acidic condition Research Journal of Chemical Sciences ______ Vol. 1(5), 85-87, Aug. (2011) International Science Congress Association but the yield is not satisfactorily detected by GC antibacterial activity of derivatives. The r esults exhibited in the table 1 show that a 4- methoxyphenyl (pyrazin methanone derivative has good a ntibacterial action. Table-1 Antibacterial activity of 4- methoxyphenyl (pyrazin yl) methanone by agar well diffusion Microorganism Inhibition Zone (mm) 4- methoxyphenyl (pyrazin- 2 methanone Staphylococcus aureus 32 mm Escherichia coli 29 mm Acknowledgement The author acknowledges with thanks the Head, Department of Physics, S. N. Arts, D. J. M. Commerce & B. N. S. Science College, Sangamner, Dist. Ahmednagar (MS), India and Director, P.D.F. Applied Research and Development Institute, Ahmednagar, (MS), India who have extended their valuable support during this study. References1. Anzai K., Isono K., Okuma K. and Suzuki S., antibiotics, questiomycins A and B., 125-132, (1960) 2. Hollstein U., Spectroscopic analysis of the and kinetic DNA binding properties actinomycin analogs, Chem. Rev., 74 3. ButenandtA. Biekert E., Kubler H., Pigments in the pupal integuments of two of cabbage white butterfly, Physiol Chem. 256 (1960) Figure-1 GC-MS of 2-amino-5- methylphenol derivatives ______ _________________________________ ______________ International Science Congress Association but the yield is not satisfactorily detected by GC -MS and esults exhibited in methoxyphenyl (pyrazin -2-yl) ntibacterial action. methoxyphenyl (pyrazin -2- by agar well diffusion method Inhibition Zone (mm) methoxyphenyl 2 -yl) methanone 32 mm 29 mm The author acknowledges with thanks the Head, Department of Physics, S. N. Arts, D. J. M. Commerce & College, Sangamner, Dist. Ahmednagar (MS), India and Director, P.D.F. Applied Research and Development Institute, Ahmednagar, (MS), India who have extended their valuable support during this study. Suzuki S., The new antibiotics, questiomycins A and B., J. Antibiot., 13, 2. Hollstein U., Spectroscopic analysis of the equilibdum and kinetic DNA binding properties of several 625-652 (1974) Biekert E., Kubler H., and Linzen B., Pigments in the pupal integuments of two colour types Physiol Chem. , 319, 238-4. Lalehzari A., Desper J. crystal structures, and antibacterial activities of copper(II) complexes derived from 1 morpholin-4- ylethylimino) methyl] transition metal chemistry 2008) 5. Yamaguchi T., Sato K., Izumi R., To Tohoku J., In Vitro a against h elicobacter pylori 161-165 (2003) 6. Uruma T., Yamaguchi H., Fukuda M., Goto H., Kishimoto T., Kamiya S., Chlamydia pneumoniae in lymphocytes, J. Med. Microbiol. 2005) 7. Kadzewski A. and naphthalene-1,5- diamine 03498-03500 (2006) 8. Ziegler J., Schuerle T., Pasierb L., Kelly C., Elamin A., Cole K. A. and Wright D. W., synthesis, characterization and of zinc (II) salicylaldimine 39, 3731(2009) 9. Leese C.L. and Rydon H.N., Some derivatives of 1:4:5 quinoxaline, J. Chem. Soc 10. 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Wt.: 214.22m/e: C, 67.28; H, 4.71; N, 13.08; O, 14.94 GC-MS of 4-methoxyphenyl(pyrazin-2-yl)methanone derivative obtained from 2,3-dihydro-5,6-dimethoxyinden-1-one.Scheme- I 4-methoxyphenyl (pyrazin-2-yl) methanone derivatives from p-methoxybenzaldehyde