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Simple and sensitive LC-ESI-MS method for estimation of dexketoprofen in plasma samples

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Author Affiliations

  • 1Synergen Bio Private Limited, Unit Nos. 101 to 104 and 309 to 311, Sai Chambers, 302, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India
  • 2Synergen Bio Private Limited, Unit Nos. 101 to 104 and 309 to 311, Sai Chambers, 302, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India
  • 3Synergen Bio Private Limited, Unit Nos. 101 to 104 and 309 to 311, Sai Chambers, 302, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India

Res.J.chem.sci., Volume 14, Issue (2), Pages 12-18, June,18 (2024)

Abstract

A simple and sensitive LC-ESI-MS assay was developed to determine the concentration of dexketoprofen in human plasma. Dexketoprofen and ketoprofen D3 (internal standard) were extracted from the plasma using solid phase extraction technique and injected onto Phenomenex Luna C18 100mm*4.6 mm, 3µm. The mobile phase consisted of Acetonitrile: 0.2% Formic Acid (65:35, v/v) and was delivered isocratically at a flow rate of 1.0 mL/min (Split 50:50 v/v). The MRM mode for dexketoprofen and internal standard ketoprofen D3 were detected at m/z 255.1→209.2 and 258.1→212.2 and the run time was 3 min. The detection limit of dexketoprofen was 50.0ng/mL, and the calibration curve was linear between 50.0 and 6000.0ng/ml. Concentrations of drugs were determined by using a validated LC-MS/MS method.

References

  1. Ameyibor E. and Stewart J.T. (1998)., HPLC determination of ketoprofen enantiomers in human serum using a nonporous octadecylsilane 1.5 microns column with hydroxypropyl beta-cyclodextrin as mobile phase additive., J. Pharm Biomed Anal., 17, 83–8. https://doi.org/10.1016/S0731-7085(97)00161-1.
  2. Ballesteros-Gómez A. and Rubio S. (2012)., Environment-responsive alkanol-based supramolecular solvents: Characterization and potential as restricted access property and mixed-mode extractants., Anal Chem., 84, 342–9. https://doi.org/10.1021/ac2026207.
  3. Bhusari V.K. and Dhaneshwar S.R. (2011)., Application of a stability-indicating tlc method for the quantitative determination of dexketoprofen trometamol in pharmaceutical dosage forms., J. Liq Chromatogr Relat Technol., 34, 2606–20. https://doi.org/10.1080/10826076. 2011.593071.
  4. Tettey-Amlalo RNO and Kanfer I. (2008)., Rapid UPLC-MS/MS method for the determination of ketoprofen in human dermal microdialysis samples., J. Pharm Biomed Anal., 50, 580–6. https://doi.org/10.1016/j.jpba.2008.09. 051.
  5. Vaghela A., Patel A., Vyas A. and Patel N. (2016)., Sample preparation in bioanalysis: A review., Int J Scientific Technol Res., 5, 6–10.
  6. Moein M.M., El Beqqali A. and Abdel-Rehim M. (2017)., Bioanalytical method development and validation: Critical concepts and strategies., J. Chromatogr B Anal Technol Biomed Life Sci. 1043, 3–11. https://doi.org/10.1016/j.jchromb.2016.09.028.
  7. Miranda H.F., Sierralta F., Aranda N., Noriega V. and Prieto J.C. (2016)., Pharmacological profile of dexketoprofen in orofacial pain., Pharmacol Rep., 68, 1111–4. https://doi.org/10.1016/j.pharep.2016.06.015.
  8. Mabrouk M.M., Hammad S.F., El-fatatry H.M. and El-Malla S.F. (2014)., Spectroscopic methods for determination of dexketoprofen trometamol and tramadol HCl., Pharm Analysis Quality Assurance., 4, 276–82.
  9. Kole PL, Venkatesh G, Kotecha J and Sheshala R. (2011)., Recent advances in sample preparation techniques for effective bioanalytical methods., Biomed Chromatogr., 25, 199–217. https://doi.org/10.1002/bmc.1560.
  10. Haq N, Iqbal M, Alanazi FK, Alsarra IA, Shakeel F. (2017)., Applying green analytical chemistry for rapid analysis of drugs: Adding health to pharmaceutical industry., Arab J Chem., 10, 777–85. https://doi.org/10. 1016/j.arabjc.2012.12.004.