@Research Paper
<#LINE#>Removal of lead and inhibition of algal growth using prodigiosin produced by serratia marcescens<#LINE#>A.M.@Shaba,S.B.@Oyeleke,O.A.@Oyewole,S.S.D.@Mohammed,A.O.@Salahudeen,C.C.@Ikekwem <#LINE#>1-6<#LINE#>1.ISCA-IRJBS-2017-041.pdf<#LINE#>Department of Biological Sciences, Niger State Polytechnic Zungeru, Niger State, Nigeria@Department of Microbiology, Federal University of Technology, Minna, Nigeria@Department of Microbiology, Federal University of Technology, Minna, Nigeria@Department of Microbiology, Kaduna State University, Kaduna State, Nigeria@Department of Microbiology, Federal University of Technology, Minna, Nigeria@Department of Microbiology, Federal University of Technology, Minna, Nigeria<#LINE#>14/5/2017<#LINE#>25/7/2017<#LINE#>This study examined the removal of lead and inhibition of Anabaena sphaerica and Oscillatoria agardhii growth using prodigiosin produced by Serratia marcescens. Inhibition of the algal growth was studied by the addition of different concentration of prodigiosin 50µl, 100µl, 150µl in 90ml of algal culture. Control was without the pigment prodigiosin. Inhibition rates were determined at the interval of 72 hours of incubation using spectrophotometer. A.sphaerica record highest level of inhibition in all concentrations. It was observed to record highest levels of inhibition at 100µg/L concentration of prodigiosin which was 76.7%, while O.agardhii was 66.3% at the same concentration. At concentration of 50µg/L A.sphaerica record of 66.3% inhibition with O.agardhii recording 64.3% while at concentration of 150µg/L it was 67.2% and 66.5% respectively. Statically analysis shows no significant differences between the rate of inhibition by the two algal species studied but there was significant difference between concentration of prodigiosin and the rate of inhibition. Removal of lead polluted soil sample was studied by the addition of different concentration of prodigiosin 50µl, 100µl, 150µl to 5g of lead polluted soil in 90ml of distilled water. The lead removal rate was determined at the interval of 4 weeks of incubation for six months using atomic absorption spectroscopy (AAS). The result shows that 100µl of prodigiosin enhance high amount of lead removal from soil. The amount of lead immobilized by the pigment at 20 weeks was 52.5% and become stable after 24 weeks. This was followed by 50µL and 150µL of prodigiosin in which there was immobilization of 41.2% and 35.3% respectively at 20 weeks of treatment.The results suggest that the red pigment inhibited cyanobacteria growth and can be used as a potential for the removal of soil polluted with lead.<#LINE#>White J., Bibb M. and Bld A. (2007).@Dependence of undecyl prodigiosin production in Streptomyces coelicolor A3 (2) involves a pathway-specific regulatory cascade.@J. Bacteriology, 179, 627-633.@No$Ajayi S.O. and Osibanjo O. (1981).@Pollution studies on Nigerian rivers. 2. Water quality of some Nigerian rivers.@Environmental Pollution, 2(2), 87-95.@Yes$Kadiri M.O. (2006).@Phytoplankton flora and physicochemical attributes of some water in Eastern Nigeria.@Nigerian Journal of Botany, 19(2), 188-200.@Yes$Khanafari Anita, Assadi Mazaheri M. and Fakhr Ahmadi F. (2006).@Review of prodigiosin, Pigmentation in Serratia marcescens.@Journal of Biological Sciences, 6(1), 1-13.@Yes$Adesalu T.A. and Nwankwo D.I. (2005).@Studies on the phytoplankton of Olero creek and parts of Benin River, Nigeria.@Ekologia, 3(2), 21-30.@Yes$Jeong H., Yim J.H., Lee C., Choi S.H., Park Y.K., Yoon S.H., Hur Cheol-Goo, Kang Ho-Young, Kim Dockyu, Hee Lee Hyun, Hyang Park Kyun, Park Seung-Hwan, Park Hong-Seog, Kum Lee Hong, Kwang Oh Tae and Kim Jihyun F. (2005).@Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent.@Nucleic Acids Research, 33(22), 7066-7073.@Yes$Kim D., Lee J.S., Park Y.K., Kim J.F., Jeong H., Oh T.K., Kim B.S. and Lee C.H. (2007).@Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396.@Applied Microbiology, 102(4), 937-944.@Yes$Pandey S., Sree A., Dash S.S., Sethi D.P. and Chowdhury L. (2013).@Diversity of marine bacteria producing beta-glucosidase inhibitors.@Microbiology of Cell Fact, 12, 35.@Yes$Tsuda K., Takamura N., Matsuyama M. and Fujii Y. (2011).@Assessment method for leaf litters allelopathic effect on cyanobacteria.@Journal of Aquatic and Plant Management, 43, 43-46.@Yes$Anderson D.M. (2009).@Approaches to monitoring, control and management of harmful algal blooms (HABs).@Ocean Coastal Management, 52(7), 342-347.@Yes$Sengco M.R. and Anderson D.M. (2004).@Controlling harmful algal blooms through clay flocculation.@Journal of Eukaryotic Microbiology, 51(2), 169-172.@Yes$Van Hullebusch E., Deluchat V., Chazal P.M. and Baudu M. (2002).@Environmental impact of two successive chemical treatments in a small shallow eutrophied lake: Part II. Case of copper sulfate.@Environmental Pollution, 120(3), 627-634.@Yes$Alam Z.B., Otaki M., Furumai H. and Ohgaki S. (2001).@Direct and indirect inactivation of Microcystis aeruginosa by UV-radiation.@Water Research, 35(4), 1008-1014.@Yes$Su J., Yang X., Zhou Y. and Zheng T. (2011).@Marine bacteria antagonistic to the harmful algal bloom species Alexandrium tamarense (Dinophyceae).@Biological Control, 56, 132-138.@Yes$Jeong S.Y., Ishida K., Ito Y., Okada S. and Murakami M. (2003).@Bacillamide, a novel algicide from the marine bacterium, Bacillus sp. SY-1, against the harmful dinoflagellate, Cochlodinium polykrikoides.@Tetrahedr. Letters, 44(43), 8005-8007.@Yes$Bai S.J., Hung L.P., Sue J.Q., Tien Y. and Zeng T.L. (2011).@Algicide effect of a novel marine actinomycete on the toxic dinoflagellate Alexandrium tamarense.@Current Microbiology, 62, 1774-1781.@Yes$Cai W., Wang H., Tien H., Tian Y., Chen F. and Zheng T.L. (2011).@Influence of a bacteriophage on the population dynamic of toxic dinoflagellate by lysis of algicidal bacteria.@Applied and Environmental Microbiology, 77(21), 7837-7840.@Yes$Mayali X. and Azam F. (2004).@Algicidal bacteria in the sea and their impact on algal blooms.@Journal of Eukaryotic Microbiology, 51(2), 139-144.@Yes$Wang X., Gong L., Liang S., Han X., Zhu C. and Li Y. (2012).@Algicidal activity of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa.@Harmful Algae, 4(2), 433-443.@Yes$Yoshinaga I., Kim M.C., Katanozaka N., Imai I., Uchida A. and Ishida Y. (1998).@Population structure of algicidal marine bacteria targeting the red tide forming alga Heterosigma akashiwo (Raphidophyceae), determined by restriction fragment length polymorphism analysis of the bacterial 16S ribosomal RNA genes.@Marine Ecology, 170, 33-44.@Yes$Wang X., Gong L., liang S., Han X.J., Zhu C. and Li Y. (2005).@Algicidal activity of rhamnolipid biosurfactants by Pseudomona aeruginosa.@Harmful Algae, 4(2), 433-443.@Yes$Imai I., Fujimaru D., Nishigaki T., Kurosaki M. and Sugita H. (2006).@Algicidal bacteria isolated from the surface of seaweeds from the coast of Osaka Bay in the Seto Inland Sea, Japan.@African Journal of Marine Sciences, 28(2), 319-323.@Yes$Lee S.O., Kato J., Takiguchi N., Kuroda A., Ikeda T., Mitsutani A. and Ohtake H. (2000).@Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28.@Applied Environmental Microbiology, 66(10), 4334-4339.@Yes$Cho J.C. and Giovannoni S.J. (2004).@Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria.@Applied Environmental Microbiology, 70(1), 432-440.@Yes$Nagayama K., Shibata T., Funjimoto K., Honjo T. and Nakamur T. (2003).@Algicidal effect of phlorotannins from the brown algae Eckloniakurome on red tide microalgae.@Aquaculture, 218(1-4), 601-611.@Yes$Jin Z.P., Luo K., Zhang S., Zheng Q. and Yang H. (2012).@Bioaccumulation and catabolism of prometryne in green algae.@Chemosphere, 87(3), 278-284.@Yes$Furstner A. (2003).@Chemistry and biology of roseophilin and the prodigiosin alkaloids: a survey of the last 2500 years.@Angew. Chemistry International Edition England, 42(31), 3582-3603.@Yes$Takamatsu S., Hodges T.W., Rajbhandari I., Gerwick W.H., Hamann M.T. and Nagle D. G. (2003).@Marine natural products as novel antioxidant prototypes.@Journal of Natural Products, 66(5), 605-608.@Yes$Dembitsky V.M., Rezanka T., Spiìzek J. and Hanus L.O. (2005).@Secondary metabolites of slime molds (Myxomycetes).@Phytochemistry, 66, 747-769.@Yes$Mekhael H. and Yousif H. (2009).@The role of red pigment produced by Serratia marcescens as antibacterial plasmid curring agent.@Journal of Duhok University, 12(1), 268-274.@Yes$Berlanaga M., Ruiz N., Hernandez-Borrell J., Montero T. and Vinas M. (2000).@Role of outer membrane in the accumulation of quinolones by Serratia marcescens.@Canadian Journal of Microbiology, 46, 716-721.@Yes
<#LINE#>Evaluation of antimicrobial activity of Neem and black pepper extracts and its efficacy in decontaminating Gutta percha cones<#LINE#>Patil@Niranjan Nandkumar,Patel@Shweta Bhise <#LINE#>7-16<#LINE#>2.ISCA-IRJBS-2017-054.pdf<#LINE#>Department of Microbiology, Patkar-Varde College, S.V. Road, Goregaon, Mumbai, India@Department of Microbiology, Patkar-Varde College, S.V. Road, Goregaon, Mumbai, India<#LINE#>12/5/2017<#LINE#>29/7/2017<#LINE#>Dental caries is the disease very common worldwide, after periodontitis. If left untreated, the entire tooth demineralizes. Root canal treatment is the solution to the untreated decays. GP cones are used to fill in the abscess cavity of the tooth. Various irrigants are used to sterilize the GP cones and cavity is like sodium hypochlorite, ethanol etc. but these chemicals come with their own disadvantages. Thus, different parts of plants are tested for their antimicrobial activity to decontaminate GP cones. In this study, neem and black pepper would be studied for their antimicrobial activity. The ethanolic extracts of neem leaves, neem bark and kernels, and black pepper along with mixture of all three materials are used to check for their antimicrobial activity against Streptococcus mutans, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. Further GP cones are dipped in different concentration of the extracts and tested against S. mutans, which is the main causative oral pathogen. The MIC was also determined to check the minimum inhibitory concentration of these extracts. Results showed that neem and black pepper both had good anti-microbial activity against the organisms used in the study but the mixture extract showed excellent cumulative anti-microbial activity.<#LINE#>Teles Ricardo, Teles Flavia, Frias&#8208;Lopez Jorge, Paster Bruce and Haffajee Anne (2013).@Lessons Learned and Unlearned in Periodontal Microbiology.@Periodontol 2000.,62(1), 95-162.@Yes$Marcenes W., Kassebaum N.J., Bernabé E., Flaxman A., Naghavi M. and Lopez A. (2013).@Global burden of oral conditions in 1990-2010: a systematic analysis.@J Dent Res., 92(7), 592-597.@Yes$Anderson C.A., Curzon M.E., Van Loveren C., Tatsi C. and Duggal M.S. (2009).@Sucrose and dental caries: a review of the evidence.@Obes Rev., 10(s1), 41-54.@Yes$Moynihan Paula (2016).@Sugars and Dental Caries: Evidence for Setting a Recommended Threshold for Intake.@AdvNutr.,7(1), 149-156.@Yes$Ghasemi Negin, Rahimi Saeed, Shahi Shahriar, Samiei Mohammad, Frough Reyhani Mohammad and Ranjkesh Bahram (2017).@A Review on Root Anatomy and Canal Configuration of the Maxillary Second Molars.@Iran Endod  J., 12(1), 1-9.@Yes$Singh Ramandeep Gambhir, Kaur Amanpreet, Singh Arshdeep, Singh Sandhu Anmol Rattan and Singh Dhaliwal Angad Prakash (2016).@Dental Public Health in India: An Insight.@J Family Med Prim Care., 5(4), 747-751.@Yes$Aas Jørn A., Paster Bruce J., Stokes Lauren N., Olsen Ingar and Dewhirst Floyd E. (2005).@Defining the Normal Bacterial Flora of the Oral Cavity.@J ClinMicrobiol., 43(11), 5721-5732.@Yes$Jyoti B.B. (2005).@Phytotherapeutics in conservative dentistry and endodontics - a review.@J Conserv Dent., 8(2), 31-39.@Yes$Cardoso C.L., Kotaka C.R., Redmerski R., Guilhermetti M. and Queiroz A.F. (2009).@Rapid decontamination of gutta percha cones with sodium hypochlorite.@J Endod. , 25(7), 498-501.@Yes$Subbiah Subba Rao C.V. and Balaji R.G. (2005).@Effect of disinfectants and glass bead size on efficacy of glass bead sterlizer.@J ConservDent., 8(3), 23-31.@Yes$de Souza R.E., de Souza E.A., Sousa-Neto M.D. and Pietro R.C. (2003).@In vitro evaluation of different chemical agents for the decontamination of gutta-percha cones.@PesquiOdontol Bras., 17(1), 75-78.@Yes$Redmerski R., Bulla J.R., Moreno T., Garcia L.B. and Cardoso C.L. (2007).@Disinfection of gutta-percha cones with chlorhexidine.@Braz J Microbiol., 38(4), 649-655.@Yes$Sequeira J.F., Jr, da Silva C.H., Cerqueira M., das D., Lopes H.P. and de Uzeda M. (1998).@Effectiveness of four chemical solutions in eliminating Bacillus subtilis spores on gutta percha cones.@Endod Dent Traumatol., 14(3), 124-126.@Yes
<#LINE#>Evaluation of ecological status of natural vegetation of Diana forest range under Jalpaiguri division, West Bengal, India<#LINE#>Sarkar@Anup Kumar,Dey@Manas,Mazumder@Mallika <#LINE#>17-33<#LINE#>3.ISCA-IRJBS-2017-056.pdf<#LINE#>Department of Botany, Dukhulal Nibaran Chandra College, Aurangabad, Murshidabad, Pin-742201, West Bengal, India and Department of Botany, Prasanna Deb Women’s College, Jalpaiguri, Pin-735101, West Bengal, India@Jurapani High School, Jurapani, Dhupguri, Jalpaiguri, Pin-735210, West Bengal, India@Department of Botany, Raiganj University, Uttar Dinajpur, Pin-733134, West Bengal, India<#LINE#>16/6/2017<#LINE#>4/8/2017<#LINE#>Forest vegetations are the most diverse in the vegetation structure and composition which directly or indirectly responsible for the existence of other ecosystem. All forest regions are being affected by several means and thus scientific and ecological monitoring is obligatory for conseration of the forest. Angiospermic plants are a typical and important component of most of the forests and contribute considerably to biodiversity. Thus proper ecological analysis of angiospermic plants provide the elementary status of the vegetation. The present investigation is aimed to reveal the phytosociological and ecological status of the angiospermic plants of Diana Forest Range under Jalpaiguri Forest Division.<#LINE#>Merigot B., Bertrand J.A., Mazouni N., Mante C., Durbec J.P. and Gaertner J.C. (2007).@A multi-component analysis of species diversity of groundfish assemblages on the continental shelf of the Gulf of Lions (north-western Mediterranean Sea).@Estuarine, Coastal and Shelf Science , 73, 123-136.@Yes$Sarkar A.K. and Mazumder M.A. (2016).@Surveillance to Evaluate the Diversity, Dominance and Community Structure of Tree Species in Nagrakata Forest Beat of Chalsa Forest Range, West Bengal, India.@Int. J. Pure App. Biosci., 4(5), 133-143.@Yes$Prain D. (1903).@Bengal Plants.@Botanical Survey of India, Kolkata, I-II.@No$Adnan M. and Holscher D. (2012).@Diversity of Medicinal Plants among Different Forest-use Types of the Pakistani Himalaya.@Economic Botany., 66(4), 344-356.@Yes$Bhattacharya S., Tiwari K.C., Mazumdar R. and Misra A.K. (1980).@Folklore medicine from district (Assam).@Bull Med Ethnobotany Res., 1, 447-460.@Yes$Borthakur S.K. and Goswami N. (1995).@Herbal Remedies from Demoria of Kamrup district of Assam in North-East India.@Fitoterapia ., 66(4), 333-340.@Yes$Das S.N., Janardhanan K.P. and Roy S.C. (1983).@Some observations on the ethnobotany of the tribes of Totopara and adjoining areas in Jalpaiguri Districts of West Bengal.@J Economic Taxonomic Botany., 4(2), 453-474.@Yes$Hajra P.K. and Boissya A.K. (1980).@Ethnobotanical notes on Miris (Missings) of Assam plain; Jain S K: Glimpses of Indian ethno botany: Oxford and IBH Publishers, New Delhi.@161-169.@No$Tabassum R. (2015).@Angiospermic Flora of Gazipur District, Bangladesh.@The University of  Dhaka.@Yes$Dey A. and De J.N. (2012).@Traditional use of medicinal plants as febrifuge by the tribals of Purulia district, West Bengal, India.@Asian Pacific Journal of Tropical Disease., S800-S803.@Yes$Fosberg F.R. and Sachet H. (1965).@Manual of Tropical Herbaria.@Regnum Veg., The Netherlands, 39.@Yes$Mishra R. (1968).@Ecology Work. Published by Mohan Primlani.@Oxford & IBH Publication Co., New Delhi.@Yes$Phillips E.A. (1959).@Methods of vegetation study. Henri Holt Co.@Inc. New York, 318.@Yes$Sarkar A.K. (2016).@Ecological Studies of Tree Vegetation of Ramshai Forest Range, Gorumara National Park, India.@International Research Journal of Biological Sciences, 5(7), 53-59.@Yes$Sarkar A.K., Dey M. and Mazumder M. (2017).@A Comparative Study of Tree Species Composition of Panjhora Forest Beat and Sipchu Forest Beat of Chalsa Forest Range, West Bengal, India.@J App Biol Biotech., 5(2), 45-52.@Yes$Shannon C.E. and Wiener W. (1963).@The Mathematical theory of Communication, University Illinois Press, Urban.@125.@Yes$Sarkar A.K. (2015).@Phytosociological studies of tree vegetation of Moraghat forest range, India.@Asian J. Biol. Life Sci., 4(3),217-220.@No$Simpson  E.H. (1949).@Measurement of Diversity.@Nature., 163, 688.@Yes$Pielou E.C. (1966).@Species Diversity and pattern diversity of in the study of Ecological; Succession.@Jour. of theoretical Biology.,10(2), 370-383.@Yes$Margalef R. (1968).@Perspective in Ecological Theory.@Uni. Of Chicago Press, 112.@Yes$Whittaker R.H. (1977).@Evolution of species diversity In land communities.@Evolutionary biology.@Yes$Lohbeck M., Poorter M., Martinez-Ramos J., Rodriguez-Velazquez M., Van Breugel and Bongers F. (2014).@Changing Drivers of Species Dominance during Tropical Forest Succession.@Functional Ecology, 28(4), 1052-1058.@Yes$Berger W.H. and Parker F.L. (1970).@Diversity of planktonic Foraminifer in deep sea Sediments.@Science, 168(3937), 1345-1347.@Yes
@Short Communication
<#LINE#>Effect of different substrate on moisture percentage of compost<#LINE#>Ahmad@Jalil,Ameen@Ayesha,Raza@Shahid <#LINE#>34-36<#LINE#>4.ISCA-IRJBS-2017-032.pdf<#LINE#>Department of Biological sciences, University of South Asia, Lahore, Pakistan@Department of Biological sciences, University of South Asia, Lahore, Pakistan@Department of Biological sciences, University of South Asia, Lahore, Pakistan<#LINE#>22/3/2017<#LINE#>19/7/2017<#LINE#>The maintenance of moisture is very important in composting process. Moisture content is a dominant parameter in composting as it is necessary for the activity of microbes. It enhances the metabolic rate of microbes and hence increases the rate of degradation. This study was designed to observe the effect of substrate on moisture percentage. The 50% moisture was optimized in all windrows containing different substrate. It was concluded from this research that the greater moisture showed by substrate composed of cow dung+ screening matter + green waste + paper and lowest was shown by waste composed of cow dung+ screening matter + green waste + saw dust. The decrease in moisture % in all treatments was observed. The decrease in moisture from 50% is a sign of compost maturity and high activity of microorganisms and better degradation.<#LINE#>Zhang N., Wu K., He X., Li S.Q., Zhang Z.H., Shen B. and Shen Q.R. (2011).@A new bioorganic fertilizer can effectively control banana wilt by strong colonization with Bacillus subtilis N11.@Plant and Soil, 344(1-2), 87-97.@Yes$Tom L., Richard H., Veeken A. and Silva T. (2002).@Moisture relationships in composting process.@Compost science and utilization, 10(4), 286-302.@Yes$Sundberg C., Yu D., Franke-Whittle I., Kauppi S., Smårs S., Insam H. and Jönsson H. (2013).@Effects of pH and microbial composition on odour in food waste composting.@Waste Management, 33(1), 204-211.@Yes$Liang C., Das K.C. and McClendon R.W. (2003).@The influence of temperature and moisture contents regimes on the aerobic microbial activity of a bio solids composting blend.@Bioresource Technology, 86(2), 131-137.@Yes$Tiquia S.M., Tam N.F.Y. and Hodgkiss I.J. (1996).@Microbial activities during composting of spent pig-manure sawdust litter at different moisture contents.@Bioresource Technology, 55(3), 201-206.@Yes$Makan A., Assobhei O. and Mountadar M. (2013).@Effect of initial moisture content on the in-vessel composting under air pressure of organic fraction of municipal solid waste in Morocco.@Iranian Journal of Environmental Health, 10, 3.@Yes
<#LINE#>Ethnomedicinal plants used by the mising tribe of Dhemaji District of Assam, India<#LINE#>Ayam@Victor Singh,Doley@Pradip,Singh@Ch. B. <#LINE#>37-43<#LINE#>5.ISCA-IRJBS-2017-058.pdf<#LINE#>Department of Botany, Rajiv Gandhi University, Rono Hills, Itanagar 79111, Arunachal Pradesh, India@Department of Botany, Rajiv Gandhi University, Rono Hills, Itanagar 79111, Arunachal Pradesh, India@Institute of Bioresource and Sustainable Development, Takyelpat, Imphal 795001, Manipur, India<#LINE#>19/6/2017<#LINE#>4/8/2017<#LINE#>Medicinal plants are extensively used as alternative therapeutic tools for the prevention and curing of various diseases and ailments. The present study is focused on the utility of certain medicinal plants on the Mising tribe of Dhemaji district of Assam, India. Dhemaji district is rich in indigenous medicinal plants due to the wide range of habitats. The ethnic people living in the remote areas of the district, depend on traditional medicine. The Misings are distributed all over the district and practised different traditional healing methods, the information of which was generated through field studies and interview with the elderly men or/and women of the community and crosschecking with the information obtained from the local herbalists. The present investigation has revealed in the documentation of a total number of 62 plant species, distributed across 38 families and 57 genera. The most important medicinal plant families were Lamiaceae, Asteraceae, Zingiberaceae, Rutaceae, Apocynaceae, Araceae, Urticaceae, Solanaceae and Piperaceae. Most frequently medicated claims were jaundice, malarial fever, cough, stomach ailments, menstrual problems, piles, tonsillitis, blood purification, and skin diseases etc. The most commonly used plant parts were the leaf, root, and rhizome. All these claims need to be subjected to both phytochemical and pharmacological investigations to discover the potentialities of these plants and plant parts as drugs.<#LINE#>Pushpangadan P. (1995).@Ethnobiology in India.@a status report. GOI, New Delhi.@Yes$MoEF (1994).@Biodiversity country studies, strategies & action plan.@Ministry of Environment & Forest, Government of India, 48.@No$Pronob G. and Islam M. (2014).@Certain Ethnomedicinal plants used by Local communities in Sivasagar District of Assam, India.@International Journal of Pharmacy & Life Sciences, 5(11), 4013-4021.@Yes$Jain S.K. and Rao R.R. (1977).@A handbook of field and herbarium methods.@New Delhi: Today and tomorrow’s printers and publishers.@Yes$Alexiades M.N. (1996).@Collecting ethnobotanical data: an introduction to basic concept and techniques. In Selected guidelines for Ethnobotanical Research: A Field Manuel.@The New York Botanical Garden, New York, 53-94.@Yes$Kanjilal U.N., Kanjilal P.C. and Das A. (1982).@A flora of Assam@Vol. I-II, Avon Book Company, Delhi. 6, 22-156.@No$Kanjilal U.N., Kanjilal P.C., De R.N. and Das A. (1982).@A flora of Assam.@Vol. III-IV, Avon Book Company, Delhi, 6, 2-100.@No$Gupta A.K., Sadasivaiah B. and Bhat G.K. (2013).@Phyla nodiflora.@The IUCN Red List of Threatened Species.e.T164053A19646880.http://dx.doi.org/10.2305/IUCN.UK.2013-1.RLTS.T164053A19646880.en.Downloaded on 04 June 2017.@No
<#LINE#>MicroRNAs Specific Primer Design using miRNA Design Tool<#LINE#>Rachh@Maulik,Desai@Piyush <#LINE#>44-46<#LINE#>6.ISCA-IRJBS-2017-061.pdf<#LINE#>Veer Narmad South Gujarat University, Surat, Gujarat, India@Veer Narmad South Gujarat University, Surat, Gujarat, India<#LINE#>27/5/2017<#LINE#>2/8/2017<#LINE#>MicroRNAs (miRNAs) are tiny (only 18-24 nucleotide long) non-coding RNAs which involved in post-transcriptional regulation of gene expression in multi-cellular organisms by affecting both translation of mRNAs and the stability. There are several different methods used for quantification of miRNAs like Northern Blotting, quantitative RT-PCR, Microarray etc. but, quantitative RT-PCR is used as the standard that is used to validate & confirmation of the results of various methods. The design of primers for miRNA qRT PCR is very much difficult because of short length of miRNA, which size is the very much near to the length of normal PCR primers. The miRNA Design Tool is based on the Universal Probe Library (ULP) probes to design primer(s) for miRNA detection. The tool is a software based and easy method for design of working primers for target specific miRNA for qRT-PCR. The application is available as online service by AstridBio.<#LINE#>E.G. (2015).@Entrez Gene: MicroRNA 145.@Retrieved 2015-01-26.@No$Götte M., Mohr C., Koo C.Y., Stock C., Vaske A.K., Viola M., Ibrahim S.A., Peddibhotla S., Teng Y.H., Low J.Y., Ebnet K., Kiesel L. and Yip G.W. (2010).@miR-145-dependent targeting of junctional adhesion molecule A and modulation of fascin expression are associated with reduced breast cancer cell motility and invasiveness.@Oncogene,  29(50),6569-6580.doi:10.1038/onc.2010.386.PMID 20818426.@Yes$UPL (2017).@Universal Probe Library.@https://lifescience. roche.com/en_in/brands/universal-probe-library.html. 05/06/2017@No$Larsson E., Fredlund Fuchs P., Heldin J., Barkefors I., Bondjers C., Genové G., Arrondel C., Gerwins P., Kurschat C., Schermer B., Benzing T., Harvey S.J., Kreuger J. and Lindahl P. (2009).@Discovery of microvascular miRNAs using public gene expression data: miR-145 is expressed in pericytes and is a regulator of Fli1.@Genome Medicine, 1(11), 108. doi:10.1186/gm108.PMC 2808743. PMID19917099.@Yes$Zhang J., Guo H., Zhang H., Wang H., Qian G., Fan X., Hoffman A.R., Hu J.F. and Ge S. (2011).@Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.@Cancer, 117(1),86-95. doi:10.1002/cncr.25522.PMC 2995010.PMID 20737575.@Yes$Sachdeva M., Zhu S., Wu F., Wu H., Walia V., Kumar S., Elble R., Watabe K. and Mo Y.Y. (2009).@p53 represses c-Myc through induction of the tumor suppressor miR-145.@Proceedings of the National Academy of Sciences of the United States of America, 106(9), 3207-12. Doi:10.1073/pnas.0808042106. PMC 2651330. PMID 19202062.@Yes$Slaby O., Svoboda M., Fabian P., Smerdova T., Knoflickova D., Bednarikova M., Nenutil R. and Vyzula R. (2007).@Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer.@Oncology, 72(5-6), 397-402. doi:10.1159/000113489.PMID 18196926.@Yes$Starczynowski D.T., Morin R., McPherson A., Lam J., Chari R., Wegrzyn J., Kuchenbauer F., Hirst M., Tohyama K., Humphries R.K., Lam W.L., Marra M. and Karsan A. (2011).@Genome-wide identification of human microRNAs located in leukemia-associated genomic alterations.@Blood, 117(2),595-607. doi:10.1182/blood-2010-03-277012.PMID 20962326.@Yes$Zsolt C., Julianna H., Zoltan S., Eva V., Zoltan H., Erzsebet S., Attila V., Balazs D., Maria B., Attila H., Balint D., Zsolt T., Laszlo N. and Balint A. (2013).@A Versatile Method to Design Stem-Loop Primer-Based Quantitative PCR Assays for Detecting Small Regulatory RNA Molecules.@PLoS One, 8(1), e55168. doi:  10.1371/journal.pone.0055168. PMCID: PMC3561390@Yes$Busk P.K. (2014).@A tool for design of primers for microRNA-specific quantitative RT-qPCR.@BMC Bioinformatics, 15, 29. doi: 10.1186/1471-2105-15-29.@Yes
<#LINE#>Detection of Arctigenin in Ipomoea cairica L. leaves: A potential drug for Japanese encephalitis<#LINE#>Srivastava@Deepa <#LINE#>47-49<#LINE#>7.ISCA-IRJBS-2017-068.pdf<#LINE#>Department of Botany, D.D.U. Gorakhpur University, Gorakhpur, Uttar Pradesh, India<#LINE#>30/1/2017<#LINE#>27/7/2017<#LINE#>Ipomoea cairica L. is a perennial herb which grows in unstable sites, such as waste-ground and roadsides in urban areas. It has been reported for several medicinal properties. Literature survey revealed the presence of phenylpropanoid compounds in this plant. One of such compound is Arctigenin. Arctigenin, is a Phenylpropanoid dibenzylbutyrolactone lignan with antinflammatory and antioxidant activities. Recent report suggests that arctigenin inhibits neuronal apoptosis and can reduce the severity of Japanese encephalitis. The presence work confirms the presence of arctigenin in cultivated plant of Ipomoea cairica leaves extract. The dried leaves sample was extracted through column chromatography with ethanol. The extracted samples were subjected to thin layer chromatography (TLC) taking Benzene: Ethyle acetate (9:1 ratio) and high performance liquid chromatography (HPLC) in methanol. Identification of the isolated compounds was done with reference standard using TLC and HPLC. The present study confirms the presence of arctigenin in leaves extract of Ipomoea cairia leaves.<#LINE#>Srivastava D. and Shukla K. (2015).@Ipomoea cairica: A medicinal weed with promising health benefits.@International J. of Research and Review, 2(5), 687-694.@Yes$Srivastava D. and Shukla K. (2015).@Antioxidant potential of Medicinal plant Ipomoea cairica (L) Sweet.@Int. J of Dev. Res., 5(4), 4255-4258.@No$Ferreira A.A., Amaral F.A., Duarte I.D.G., Oliveira P.M., Alves R.B., Silveira D., Azevedo A.O., Raslan D.S. and Castro M.S.A. (2006).@Antinociceptive effect from Ipomoea cairica extract.@J. Ethnopharmacol., 105, 148-153.@Yes$Han B.H., Kang Y.H., Yang H.O. and Park M.K.(1994).@A butyrolactone lignan dimer from Arctium lappa.@Phytochemistry, 37(4), 1161-1163.@Yes$Zhou X., Zhang H., Ge L., Gong H. and Tian S. (2011).@Determination of Arctiin and Arctigenin contents in Arctium tomentosum Mill. By HPLC Method.@Journal of Chemistr., 8(S1), S372-S376.@Yes$Song J., Li A., Xia Y., Gao Z., Zou S.F., Kong L., Yao Y.J., Jiao Y.N., Yan Y.H., Li S.H., Tao Z.Y., Lian G., Yang J.X. and Kang T.G. (2016).@Arctigenin Treatment Protects against Brain Damage through an Anti-Inflammatory and AntiApoptotic Mechanism after Needle Insertion.@Front. Pharmacol., 7(182), 1-16.@Yes$Han H.Y., Kee J.Y., Kim D.S., Mun J.G., Jeong M.Y., Park S.H., Choi B.M., Park S.J., Kim H.J., Um J.Y. and Hong S.H. (2016).@Arctigenin Inhibits Lung Metastasis of Colorectal Cancer by Regulating Cell Viability and Metastatic Phenotypes.@Molecules, 21(9), 1135.@Yes$Srivastava D. and Shukla K. (2017).@Arctigenin, A plant Lignan with tremendous potential: A review.@International Journal of Current Research, 9(4), 50232-50237.@No$Li X.M., Miao Y., Su Q.Y., Yao J.C., Li H.H. and Zhang G.M. (2016).@Gastroprotective effects of arctigenin of Arctium lappa L. on a rat model of gastric ulcers.@Biomedical Reports, 5(5), 589-594.@Yes$Huang J., Xiao L., Wei J.X., Shu Y.H., Fang S.Q., Wang Y.T. and Lu X.M. (2017).@Protective effect of arctigenin on ethanol induced neurotoxicity in PC 12 cells.@Mol. Med. Rep., 15(4), 2235-2240@Yes$Zhu Zhiyuan, Yan Jianming, Jiang Wei, Yao Xin-gang, Chen Jing, Chen Lili, Li Chenjing, Hu Lihong, Jiang Hualiang and Shen Xu (2013).@Arctigenin effectively ameliorates memory impairment in Alzimer’s disease model mice targeting both &#945;-amyloid production and clearance.@Journal of Neuroscience, 33(32), 13138-13149.@Yes$Swarup V., Ghosh J., Mishra M.K. and Basu A. (2008).@Novel strategy for treatment of Japanese encephalitis using arctigenin, a plant lignan.@J. Antimicrob Chemother., 61(3), 679-688.@Yes$Srivastava D. (2016).@Global Scenarion of Abtiviral Drugs for Japanese Encephalitis.@Research Journal of Recent Sciences, 5(10), 1-5.@No
@Short Review Paper
<#LINE#>Review of Hafeez, Shaik. and J. Chapla., Treatment of Naja Naja (King Cobra) Snakebites in Tribal Areas of Adilabad District, India, by Hakim<#LINE#>Harit@Daya Nand <#LINE#>50-51<#LINE#>8.ISCA-IRJBS-2017-034.pdf<#LINE#>Department of Zoology, Government Champhai College, Mizoram-796 321, India<#LINE#>26/3/2017<#LINE#>26/7/2017<#LINE#>Treatment of snakebite by the ‘Hakims’ and by herbal treatments are suggested in many research paper, many of them are without any scientific proof and do not confirm the authenticity of the treatment. Several papers suggest the treatment of snake bite, without any consideration of whether snake is poisonous or non poisonous. Author has reviewed the paper very carefully, which is not only interesting but encouraging to discover herbal treatment for snake bite, by Hafeez, Shaik. and J. Chapla., Treatment of Naja Naja (King Cobra) Snakebites in Tribal Areas of Adilabad District, India, by Hakim. International Research Journal of Biological Sciences Vol. 4(3), 1-5, March (2015).<#LINE#>Das I. (2008).@A pictographic Guide to Snakes and other reptiles of India.@Om Books International, New Delhi, pp 53 & 55. ISBN : 81-87108-35-5.@No$Sharma R.C. (2003).@Hand Book of Indian Snakes.@Zoological Survey of India, Kolkata, 196-199, ISBN : 81-8171-16-9.@Yes$Smith M.A. (2003).@Hand Book of Indian Snakes.@Cosmo Publications, New Delhi, 427-436, ISBN : 81-7755-743-2.@No$Wall F. (2000).@The Poisonous Terrestrial Snakes.@Asiatic Publishing House, Delhi, 23-29, ISBN : 81-87067-35-7.@No$Whitaker R and Ashok Captain (2004).@Snakes of India the field Guide.@Draco Books Chennai, India, 304-312. ISBN: 81-901873-2-5.@Yes$Sainkhediya Jeetendra and Aske Dilip Kumar (2012).@Ethno-medicinal plants used by tribal communities for the treatment of Snake bite in West Nimar, MP.@ISCA Journal of Biological Sciences, 1(2), 77-79.@Yes$Menon Jaideep, Joseph J.K. and Kulkarni Kruntik (2007).@Treatment of Snake bite – A resume.@Cobra, 1(4), 1-21.@No