@Research Paper
<#LINE#>Extraction, purification and characterization of pyocyanin produced by Pseudomonas aeruginosa and evaluation for its antimicrobial activity<#LINE#>Popy @Devnath,Md. Kamal @Uddin,Forkan @Ahamed,Md. Towhid @Hossain,Mohammed Abul @Manchur <#LINE#>1-9<#LINE#>1.ISCA-IRJBS-2017-029.pdf<#LINE#>Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh@Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh@Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh@Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh@Department of Microbiology, Faculty of Biological Sciences, University of Chittagong, Chittagong 4331, Bangladesh<#LINE#>13/3/2017<#LINE#>5/4/2017<#LINE#>Pseudomonas aeruginosa, a gram negative bacteria, which exerts broad antagonistic activity against other bacterial and fungal pathogens through the production of a secondary metabolite-pyocyanin. In the present study, various clinical samples were taken for P. aeruginosa isolation. From sixteen primary P. aeruginosa isolates, five isolates (PS1, PU5, PU8, PU10 and PP3) were selected, on the basis of pigmentation in cetrimide agar. The screening for antimicrobial activity of P aeruginosain cross streak method showed that Staphylococcus aureus (ATCC 6538), Salmonella enterica (NCTC 6017), and Bacillus cereus (ATCC 14579) were sensitive, Escherichia coli (ATCC 8739) was intermediate, and K. pneumoniae (ATCC 43816) was resistant to the inhibitory action of the selected P. aeruginosa isolates. The antimicrobial pigment pyocyanin was extracted from culture broth following solvent extraction method. Purification of the pigment was done by column and thin layer chromatography. The Rf value was found around 0.81 for all the extracted pigment solution. Confirmation of the pigment as pyocyanin was done through FTIR, and UV-visible spectrophotometric analysis. FTIR analysis revealed different functional groups (-OH, -C=N, -CH3 etc.) which belongs to the aromatic structure of pyocyanin. In UV-Vis spectrophotometric analysis, a maximum absorption was observed at 270-275nm.The modification of media composition enabled to increase pyocyanin production and the highest amount was produced by the isolate PU10 in Medium-B having a concentration of 9.45 &#956;g/mL. The antimicrobial activity of the purified pyocyanin pigment against different test organism showed that highest concentration of pyocyanin (25 &#956;g/mL) was needed to produce zone of inhibition in case of E. coli, and the lowest (5 &#956;g/mL) was for S. aureus.<#LINE#>Lyczak J.B., Cannon C.L. and Pier G.B. (2000).@Establishment of Pseudomonas aeruginosa infection: lessons from a versatile opportunist.@Microbes Infect., 2(9), 1051-1060.@Yes$Delden C.V. and Iglewski B.H. (1998).@Cell-to-cell signaling and Pseudomonas aeruginosa infections.@Emerg Infect. 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(2014).@Pyocyanin: production, applications, challenges and new insights.@World J.Microbiol.Biotechnol.,30(4), 1159-1168. DOI: 10.1007/s11274-013-1552-5@Yes$King E.O., Ward M.K. and Raney D.E. (1954).@Two simple media for the demonstration of pyocyanin and fluorescin.@J. Lab.Clin. Med., 44(2), 301-307.@Yes$Hassett D.J., Charniga L., Bean K., Ohman D.E. and Cohen M.S. (1992).@Response of Pseudomonas aeruginosa to pyocyanin: mechanisms of resistance, antioxidant defenses, and demonstration of a manganese-cofactored superoxide dismutase.@Infect. Immun., 60(2), 328-336.@Yes$Friedheim E. and Michaelis L. (1931).@Potentiometric study of pyocyanine.@J. Biol. Chem., 91(1), 355-368.@Yes$Silverstein R.M., Bassler G.C. and Morrill T.C. (1981).@Spectrometric Identification of Organic Compounds.@Fourth Edition. John Wiley & Sons Inc. ISBN-13: 978-0471863557@Yes$Boulette M.L., Baynham P.J., Jorth P.A., Kukavica-Ibrulj L., Longoria A., Barrera K., Levesque R.C. and Whiteley M. (2009).@Characterization of Alanine Catabolism in Pseudomonas aeruginosa and Its Importance for Proliferation In Vivo.@J. Bacteriol., 191(20), 6329-6334.DOI: 10.1128/JB.00817-09@Yes$Mac-Faddin J.F. (1985).@Media for Isolation - Cultivation - Identification - Maintenance of Medical Bacteria.@Williams and Wilkins. ISBN: 0683053167@Yes$Ramalho R., Cunha J., Teixeira P. and Gibbs P.A. (2002).@Modified Pseudomonas agar:  new differential medium for the detection/ enumeration of Pseudomonas aeruginosa in mineral water.@J.Microbiol.Methods, 49(1), 69-74.@Yes$Palumbo S.A. (1972).@Role of iron and sulfur in pigment and slime formation by Pseudomonas aeruginosa.@J.Bacteriol., 111(2), 430-436.@Yes$Hassan H.M. and Fridorich I. (1980).@Mechanism of the antibiotic action of pyocyanine.@J.Bacteriol., 141(1), 156-163.@Yes$Kerr J.R., Taylor G.W., Rutman A., Høiby N., Cole P.J. and Wilson R. 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<#LINE#>Seasonal variations in the bottom sedimental macro-nutrients and its impact on the bio-chemical profile of fresh water molluscan Pila globosa<#LINE#>R. @Ranjani,A.S. @Maheswari <#LINE#>10-14<#LINE#>2.ISCA-IRJBS-2017-030.pdf<#LINE#>Research Department of Zoology, Seethalakshmi Ramaswami College (Women), Thiruchirappalli - 620 002, India @Research Department of Zoology, Seethalakshmi Ramaswami College (Women), Thiruchirappalli - 620 002, India <#LINE#>17/3/2017<#LINE#>24/4/2017<#LINE#>The study was carried out to determine the nutrient content of important molluscs. Pila globosa, the commonly available fresh water molluscan species was selected for the ananlysis, and to investigate the size and seasonal variation of Protein, Carbohydrate, and Lipid contents from Koothaippar wetlands, between August 2013 to January 2014, covering three Seasons, Pre – Monsoon (August and September), Monsoon (October – December) and Post Monsoon (January). Low amount of protein was recorded in premonsoon season (0.0933 mg) and maximum in Postmonsoon season (0.1363mg). Minimum amount of carbohydrate was recorded in premonsoon (0.0733mg) and maximum in Postmonsoon (0.0933mg). Minimum amount of lipid present in Monsoon and Postmonsoon season (0.08mg). Total quantity of protein in region I, and low values of protein present in monsoon (0.081mg) and high during Postmonsoon season (0.153mg). The low amount of carbohydrate was present in premonsoon season 0.0643mg and maximum in monsoon season (0.161mg) in region II. This paper highlights the preliminary investigation of the bottom sedimental macro nutrients and the influence of these sedimental characteristics on the nutritive profile of Pila golobosa in two regions of Koothaippar lake ecosystem.<#LINE#>Padidela Swapna and Thummala R.R. (2015).@Proximate, Amino acid, Fatty acid and mineral analysis of Bivalve Parreysia Cylindrica from Waddepally and Kaleshwaram Lake.@World Journal of Pharmacy and Pharmaceutical sciences, 4(4), 1388-1401.@Yes$Subba Rao N.V. and Dey A. (1989).@Freshwater Mollusca in Aquaculture.@Handbook of Freshwater Molluscs of India, 225-232.@Yes$Thorp James H. and Covich Alan P. (2009).@Ecology and classification of North American Freshwater Invertebrate.@Academic Press, 331-429.@Yes$Rao Subba N.V. (1993).@Freshwater Molluscs of India.@Recent Advances in Freshwater Biology. New Delhi. Anmol Publication, 2, 187-202.@Yes$Raveendran (2016).@Molluscan Diversity in Keezhathottam of Thanjavur District, Tamil Nadu, India with special Reference to Gastropods and Bivalves.@International Journal of Zoology and Applied Biosciences, 1, 141-143.@No$Malleswar Dharanikota (2014).@Activities of selected enzymes in various Tissues of Pila globosa under Heat shock.@International journal of Environment sciences, 3(6), 11-15.@No$Rao Subba N.V. (1989).@Handbook freshwater molluscs of India.@Zoological Survey of India. The Radiant Process Private Limited. Calcutta, 1-283.@Yes$Nazir Ahmed T.A. (2007).@A practical manual for M.Sc. Zoology.@M.M.A. Publication. 269-A, Bharathisalai, Karikkadu, pattukkottai, 159-161.@No$Maheswari A.S. and Thiyagesan K. (2014).@Macro nutrients (N, P & K) Profile of the bottom sediments of koothaippar wetlands in Relation to sediment pH, electrical conductivity, Atmospheric and water temperature.@Biochem. cell. Arch., 14(2), 343-347.@No
<#LINE#>Status and diversity of avian fauna in and around Bordi region, west coast of India<#LINE#>Kadam @Surendra S.,Avadhesh Shashi @Dhar <#LINE#>15-18<#LINE#>3.ISCA-IRJBS-2017-035.pdf<#LINE#>Dept. of Zoology, N.B Mehta College of Science & Commerce, Bordi, Tal. Dahanu, Dist. Palghar, 401 702, MS, India@Dept. of Zoology, N.B Mehta College of Science & Commerce, Bordi, Tal. Dahanu, Dist. Palghar, 401 702, MS, India<#LINE#>2/4/2017<#LINE#>25/4/2017<#LINE#>This is the first report on avian diversity in and around Bordi region. Bordi is a coastal village situated in Palghar District of Maharashtra, which lies along state’s border with Gujarat. Bordi is about 130 km from busting city of Mumbai and 40 km away  from Vapi which is famous for pharmaceutical and chemical companies and factories. Bordi is located between 200 09’ 00” N latitude and 720 43’ 00” E longitude with average elevation is about 12m. Total 6 sites were selected for study of Avian fauna including, Bordi, Jambugaon, Aswali, Gholwad, Zai and Borigaon. The study period was from June 2013 to December 2016.  Record images were taken using a Canon SX50HS camera and observations were also made through Olympus 10x50 DPS R binocular. During the study period, a total of 48 Avian species belonging to 14 orders and 28 Families with Order Passeriformes having maximum species of 15 were recorded and 6 species under both Pelecaniformes and Charadriiformes and Family Ardeidae with 5 species under it followed by Muscicapidae with 4 species under it. Some uncommon sightings were observed like a pair of Woolly Necked Stork and very elusive and shy Slaty Breasted Rail.<#LINE#>Monga S. (2003).@Birds of Mumbai.@India book house Pvt.,Ltd. 176.@No$Verma A., Balachandran S.,Chaturvedi N. and Patil V. (2004).@A preliminary report on the biodiversity of Mahul Creek, Mumbai, India with special reference to Avifauna.@Zoo’s print journal, 19(9), 1599-1605.@Yes$Pawar P.R. (2011).@Species diversity of Birds in Mangroves of Uran (Raigad), Navi Mumbai, Maharashtra, West coast of India.@Journal of experimental science, 2(10), 73-77.@Yes$Walmiki N., Karangutkar S., Yengal P., Pillai R., Ajgaonkar P., Singh N. and Sagre P. (2013).@Avian diversity in and around Bassein Fort and creek, district Thane, Maharashtra.@International Journal of Advanced Research, 1(3), 73-85.@No$Nitsure S.R. (2002).@Study of avifauna at Thane creek near Rituchakkra nature park.@Master’s thesis submitted to the Indian Institute of Ecology and environment , New Delhi, 265.@Yes$Quadros G. (2001).@Study of inter-tidal fauna of Thane creek.@Ph.D Thesis, University of Mumbai, 293.@Yes$Singh R.B. (2016).@Beautiful Avifauna of Waghoba Forest of Palghar Maharashtra.@Indian Journal of Research, 5(12), 495-498.@No$Sinnarkar K., Hule A.S., Dalvi R.S. and Kamath V. (2013).@Avian diversity in Mahim Bay, Mumbai.@Proc. National Conference on Biodiversity: Status & Challanges in Conservation-‘FAVEO’: 1-6.@No$Lad D. and Patil S. (2015).@Status and Diversity of Avian fauna in the estuarine wetland area of Bhayander and Naigaon, Maharashtra, India.@Bioscience Discovery,6(1), 39-44.@Yes$Ali S. and Dhillon S. (2003).@Ripley- Handbook of Birds of India and Pakistan.@Oxford University Press, 4, 310.@No$Abdulali H. (1981).@Checklist of the Birds of Borivali National park with notes on their status.@Bombay, BNHS.@Yes$Kulkarni A.N., Kanwate V.S. and Deshpande V.D. (2005).@Birds in and around Naned City, Maharashtra.@A Zoos Print Journal,20(11), 2076-2078.@Yes$Subramaniya S. (2005).@Heronries of Tamil Nadu.@Indian Birds, 1(6), 126-140.@Yes
@Short Communication
<#LINE#>Effect of different substrate on organic matter during composting process<#LINE#>Ayesha @Ameen,Jalil @Ahmad,Shahid @Raza <#LINE#>19-21<#LINE#>4.ISCA-IRJBS-2017-033.pdf<#LINE#>Department of Biological sciences, University of South Asia, Lahore Pakistan@Department of Biological sciences, University of South Asia, Lahore Pakistan@Department of Biological sciences, University of South Asia, Lahore Pakistan<#LINE#>22/3/2017<#LINE#>24/4/2017<#LINE#>Organic matter is important to assess the maturity of compost prepared by using organic waste. The decrease in organic matter content reveals that the degradation rate is active. Many microbes utilize this organic matter by mineralization. The study was designed to check the effect of different organic wastes used as a substrate on organic matter. It was concluded from this study that different organic waste substrate show variation in organic matter during the process of composting. The highest value of organic matter was observed in experimental heap with humic acid addition and the lowest was observed in control heap.<#LINE#>Ciavatta C., Govi M., Pasotti L. and Sequi P. (1993).@Changes in organic matter during stabilization of compost from municipal solid wastes.@Bioresource Technology, 43(2), 141-145.@No$Polprasert C. (1989).@Organic waste recycling.@SciTech Connect.@Yes$Bernai M.P., Paredes C., Sanchez-Monedero M.A. and Cegarra J. (1998).@Maturity and stability parameters of composts prepared with a wide range of organic wastes.@Bioresource Technology, 63(1), 91-99.@No$Nakasaki K., Nag K., andKarita S. (2005).@Microbial succession associated with organic matter decomposition during thermophilic composting of organic waste.@Waste management & research, 23(1), 48-56.@No$Burke I.C., Elliott E.T. and Cole C.V. (1995).@Influence of macroclimate, landscape position, and management on soil organic matter in agroecosystems.@Ecological applications, 5(1), 124-131.@Yes$Tognetti C., Mazzarino M.J. and Laos F. (2007).@Improving the quality of municipal organic waste compost.@Bioresource Technology, 98(5), 1067-1076.@Yes$Pascual J.A., Garcia C. and Hernandez T. (1999).@Comparison of fresh and composted organic waste in their efficacy for the improvement of arid soil quality.@Bioresource Technology, 68(3), 255-264.@No$Albiach R., Canet R., Pomares F. and Ingelmo F. (2001).@Organic matter components and aggregate stability after the application of different amendments to a horticultural soil.@Bioresource Technology, 76(2), 125-129.@Yes$Castaldi P., Alberti G., Merella R. and Melis P. (2005).@Study of the organic matter evolution during municipal solid waste composting aimed at identifying suitable parameters for the evaluation of compost maturity.@Waste Management, 25(2), 209-213.@Yes$Iqbal M.K., Khan A.H.M.E.D., Nadeem A. and Hussnain A. (2012).@Comparative study of different techniques of composting and their stability evaluation in municipal solid waste.@Journal of Chemical Society Pakistan, 34(2), 273-282.@Yes