@Research Paper <#LINE#>Assessment of airborne fungal pollution in a hospital room<#LINE#>Jyoti @Gaur,Kavita @Naruka <#LINE#>1-6<#LINE#>1.ISCA-IRJBS-2017-089.pdf<#LINE#>Department of Science, Aishwarya College of Education, Jodhpur, Rajasthan, India@Department of Zoology, J.N.V. University, Jodhpur, Rajasthan, India<#LINE#>22/9/2017<#LINE#>25/11/2017<#LINE#>Hospitals and other health care facilities are designed to fight various diseases and to provide complete patient care. Now days it has been a tremendous increase in hospital acquired infections, especially those caused by fungi. To reduce these incidences routine proper monitoring is required. Therefore, present study was performed to detect the current airborne fungal load and the influence of meteorological factors on their concentrations in a hospital outdoor patient room. A total of 8 fungal species were isolated among which Aspergillus showed dominance. Quantitative and qualitative seasonal variation in airborne fungal load was also observed.<#LINE#>Centeno S. and Machado S. (2004).@Assessment of airbone mycoflora in critical areas of the principal hospital of Cumaná, state of Sucre, Venezuela.@Invest Clin., 45(2), 137-144.@Yes$Rainer J., Peintner U. and Pöder R. (2001).@Biodiversity and concentration of airbone fungi in a hospital environment.@Mycopathologia, 149, 87-97.@Yes$Sanca S., Asan A., Otkun M.T. and Ture M. (2002).@Monitoring indoor airborne fungi and bacteria in the different areas of Trakya University hospital, Edirne, Turkey.@Indoor Built Environ., 11(5), 285-292.@Yes$Pfaller M.A. (1996).@Nosocomial candidiasis: emerging species, reservoir, and modes of transmission.@Clin Infect Dis., 22, 89-94.@Yes$Pini G., Faggi E., Donato R. and Fanci R. (2005).@Isolation of Trichosporon in a hematology ward.@Mycoses, 48(1), 45-49.@Yes$Wang C.Y., Wu H.D. and Hsueh P.R. (2005).@Nosocomial transmission of cryptococcosis.@N Engl J Med., 352, 1271-1272.@Yes$Gould J.C. (1970).@Airborne pathogenic bacteria in a tissue transplant unit.@In: Silver IH (Ed): Aerobiology. Academic Press, London, 62-76.@Yes$Li C.S. and Hou P.A. (2003).@Bioaerosol characteristics in hospital clean rooms.@Sci Total Environ., 305(1-3), 169-176.@Yes$Panagopoulu P., Filioti J., Petrikkos G., Giakouppi P., Anatoliotaki M., Farmaki E., Kanta A., Apostolakou H., Avlami A., Samonis G. and Roilides E. (2002).@Environmental surveillance of filamentous fungi in three tertiary care hospitals in Greece.@J Hosp Infect., 52(3), 185-191.@Yes$Pei-Chih W., Huey-Jen S. and Chia-Yin L. (2000).@Characteristics of indoor and outdoor airbone fungi at suburban and urban homes in two seasons.@The Sci of the Total Environ., 253(1-3), 111-118.@Yes$Naruka K. and Gaur J. (2013).@Microbial air contamination in a school.@Int. J. Curr. Microbiol. App. Sci., 2(12), 404-410.@Yes$Obbard J.P. and fang L.S. (2003).@Airborne concentrations of bacteria in a hospital environment in Singapore.@Water, Air & Soil Pollution., 144(1-4), 333-341.@Yes$Frey D., Oldfield R.J. and Bridger R.C. (1979).@A colour atlas of pathogenic fungi.@Wolfe Medical Publications Ltd. Holland.@Yes$Aegerter B.J., Nuñez J.J. and Davis R.M. (2003).@Environmental factors affecting rose downy mildew and development of a forecasting model for a nursery production system.@Plant Dis., 87(6), 732-738.@Yes$Ekhaise F.O., Ighosewe O.U. and Ajakpovi O.D. (2008).@Hospital indoor airborne microflora in private and government owned hospitals in Ben in City, Nigeria.@World Journal of Medical Sciences, 3(1), 19-23.@No$Qudiesat K., Abu-Elteen K., Elkarmi A., Hamad M. and Abussaud M. (2009).@Assessment of airborne pathogens in healthcare settings.@African Journal of Microbiology Research, 3(2), 66-76.@Yes$Martone W.J. (1992).@Incidence and nature of endemic and epidemic nosocomial infections.@hospital infections, J.V Bennet and Brachman, editors, Boston: Little Brown and Co., 577-596.@Yes$Augustowska M. and Dutkiewicz J. (2006).@Variability of Airborne Microflora in a hospital ward within a period of one year.@Ann Agric Environ Med., 13, 99-106.@Yes$Bhatia L. and Vishwakarma R. (2010).@Hospital indoor airborne microflora in private and government owned hospital in Sagar city, India.@World Journal of Medical Sciences., 5(3), 65-70.@Yes$Panagopoulu P., Filioti J., Petrikkos G., Giakouppi P., Anatoliotaki M., Farmaki E., Kanta A., Apostolakou H., Avlami A., Samonis G. and Roilides E. (2002).@Environmental surveillance of filamentous fungi in three tertiary care hospitals in Greece.@J Hosp Infect., 52(3), 185-191.@Yes$Lacey J. and Dulkiewicz J. (1994).@Bioaerosols and occupational lung disease.@J. Aerosol Sci., 25(8), 1371-1404.@Yes$Holmberg K. (1987).@Indoor mould exposure and health effects.@Volatile Organic Compounds, Combustion Gases, Particles and Fibers, Microbiological Agents., Institute for Water, Soil and Air Hygiene, Berlin. Indoor Air, 87(1), 637-642.@Yes$Dutkiewicz J. (1997).@Bacteria and fungi in organic dust as potential health hazard.@In: Midtgård U, Poulsen OM (Eds): Waste Collection and Recycling – Bioaerosol Exposure and Health Problems. Proceedings of an International Meeting held in Køge, Denmark, 13-14 September 1996. Ann Agric Environ Med., 4, 11-16.@Yes$Herman L.G. (1980).@Aspergillus in patient care areas.@Ann NY Acad Sci., 353, 140-146.@Yes$Ingold C.T.C.T. (1971).@T Fungal spores. Their libération and dispersal.@T Fungal spores. Their liberation and dispersal, 4, 302.@Yes$Katial P.K., Zhang Y., Jones R.H. and Dyer P.D. (1997).@Atmospheric mould spores count in relation to meteorological parameters.@International Journal of Biometeorology., 41(1), 17-22.@Yes$Mitakakis T., Clift A. and Mcgee P.A. (2001).@The effect of local cropping activities and weather on the airborne concentra¬tion of allergenic Alternaria spores in rural Australia.@Grana., 40, 230.@Yes$Azab M.M., Boghdadi G.S., Gerges M.A., Abd-Elsalam S.F. and Elnaggar Y.A. (2016).@Airway colonization with Alternaria and Cladosporium spp. in fungi-sensitized asthma patients in Sharkeya, Egypt.@Egyptian Journal of Medical Microbiology, 25(3), 103-108.@Yes$Akerman M., Valentine-Maher S., Rao M., Taningco G., Khan R., Tuysugoglu G. and Joks R. (2003).@Allergen sensitivity and asthma severity at an inner city asthma center.@J. Asthma., 40(1), 55-62.@Yes <#LINE#>Assessment of diversity and threats of avifauna in and around Sonavade village (Tarf-Kalsuli), Dist- Sindhudurg, Maharashtra, India<#LINE#>Pritesh @Ghadigaonkar,Akshay @Pandirkar,Johnson @Varkey,Prajal @Jangam,Pradnya @Bandekar,Prakash @Khedekar,Omkar @Patil <#LINE#>7-14<#LINE#>2.ISCA-IRJBS-2017-090.pdf<#LINE#>Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India@Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India@VIVA College of Arts, Commerce and Science, Viva College Road, Virar West, Vasai – 401303, MS, India@Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India@Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India@Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India@VIVA College of Arts, Commerce and Science, Viva College Road, Virar West, Vasai – 401303, MS, India<#LINE#>28/9/2017<#LINE#>26/11/2017<#LINE#>The present investigation was carried out in and around Sonavade village, Tarf-Kalsuli, Tal- Kudal, Sindhudurg district of Maharashtra. Sonavade village is located in Sahyadri mountain ranges of Western Ghat. The study area is in the vicinity of the Dajipur Wildlife Sanctuary. During the study period of May 2015 to April 2017 a total number of 136 species from 16 orders and 41 families were recorded. As per the observations of the study conducted, avifauna order Passeriformes is dominant in study area (56%), including 76 bird species in 18 families followed by Coraciiformes (7%), Accipitriformes (6%), Apodiformes, Columbiformes, Piciformes, Galliformes each 4%. Psittaciformes 3%, Capriulgiformes, Charadriiformes, Strigiformes each 2% and Trogoniformes, Bucerotiformes, Falconiformes, Suliformes each 1%. According to ‘The IUCN Redlist of Threatened Species List’ 130 species of birds observed during the study are found to be listed in Least Concern category, while Chloropsis cochinchinensis, Buceros bicornis, Anthracoceros coronatus, Sterna aurantia and Psittacula eupatria are listed in Near Threaten category (NT). Indian Vulture (Gyps indicus) Critically Endangered (CR) species was also observed in hilly part of the area under study. Geographical location of study area provides a healthy habitat for birds. Study area is rich in bird diversity but anthropogenic development, loss of habitat and poaching/hunting are the main threats to the avifauna in the study area. This study was conducted to prepare a baseline data for this region which will be referral document for conservation and research related activities undertaken in future.<#LINE#>Grimmett R., Inskipp C. and Inskipp T. (2013).@Pocket guide to the Birds of Indian subcontinent.@Oxford university press, New Delhi, India, 1-226, ISBN: 9781408162644@No$Pritesh Ghadigaonkar (2016).@Avian diversity in and around Ajoba fort, Thane District, Maharashtra.@International research Journal of biological science, 5(2), 7-15.@No$Farheen Khan (2016).@Seasonal variation of avifaunal diversity of wetland near new VIVA college, Virar West, District Palghar, Maharashtra India.@International research Journal of Environmental science, 5(11), 47-55.@No$Pawar P.R. (2011).@Species diversity of birds in mangroves of Uran (Raigad), Navi Mumbai, Maharashtra, West coast of India.@Journal of Experimental Sciences, 2(10), 73-77.@Yes$Walmiki N. (2013).@Avian diversity in and around Bassein Fort and Creek, Dist. Thane, Maharashtra.@International Journal of Advanced Research, 1(3), 73-85.@No$Dekate H.M. and Baviskar R.M. (2016).@Biodiversity of avifauna in Mumbri creek of south Konkan, Deogad Taluka Maharashtra.@Int. J. of Life Science, 4, 145-147.@Yes$Patil A.J., Rao G.B., Shirke B., Babu S. and Quadros G. (2015).@Diversity of avifauna in the shallow pat lake in Kudal taluka of sindhudurga district.@UGC sponsored National seminar on@Yes$Varkey J., Pandirkar A.H., Fernandes B., Pathak K., Khadye P. and Ghadigaonkar P. (2015).@Threats to the existing diversity of avifauna of Gogte salt plant, Mumbai.@Suburb Proceedings of the UGC Sponsored National Seminar on Wetlands: Present Status, Ecology and Conservation, India, 1-8.@Yes$Avibase-Bird checklist of the world (Sindhudurga) (2017), http://avibase.bsc eoc.org/checklist.jsp?region=INswmhsi&list=howardmoore, 18 Aug 2017@undefined@undefined@No$Indian biodiversity portal (2017).@Checklist of birds of Radhanagari Wildlife sanctuary.@http://oldwww.wii.gov.in/nwd_birds/rad.htm, 18 Aug 2017@No$Ali S. and Dillon R. (2003).@A pictorial Guide to the Birds of the Indian Subcontinent, Oxford, India.@1-466, ISBN: 0195665236@No$Bikram Grewal (2016).@A pictorial field guide to Birds of India, pakistan, Nepal, Bhutan, Shrilanka and Bangladesh.@Om Book International, India, 789, ISBN: 10987654321@No <#LINE#>Properties of spray dried liquid jaggery powder prepared using plant mucilage clarificant<#LINE#>Lava @Chikkappaiah,Harish @Nayaka M.A.,Mahadevaiah@.,Venkatesh @K.S.,Dinesh @Kumar <#LINE#>15-22<#LINE#>3.ISCA-IRJBS-2017-094.pdf<#LINE#>Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India@Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India@Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India@Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India@Department of Chemistry, Bipin Bihari Science PG College, Jhansi, UP, India<#LINE#>9/10/2017<#LINE#>23/11/2017<#LINE#>The effects of different plant mucilage clarificants and packaging materials on the characteristic properties of spray dried liquid jaggery (SDLJ) powder during storage were determined. Three different liquid jaggery were prepared using plant mucilage from Aloe vera, fenugreek (Trigonella foenum-graecum L.) and flax seeds (Linumus itatissimum L.) as clarificants, in addition control jaggery prepared without using clarificants. No preservatives were used during preparation of liquid jaggery. Spray drying of liquid jaggery was performed at an inlet air temperature of 160°C and outlet temperature of 80°C with atomising disk speed of 32000 rpm using maltodextrin as carrier. The prepared SDLJ powder was packed in different covers made up of low density polyethylene (LDPE), high density polyethylene (HDPE) and aluminium pouches and stored for six months for further analysis. At every two months interval parameters such as moisture content (%), bulk density (g/cm3), hygroscopicity (%), solubility (sec) and total reducing sugars (%) of jaggery samples were evaluated. Comparative study indicated that control SDLJ powder had poor response for all the parameters during storage in all packaging modes. Plant mucilage clarificants treated jaggery samples showed better shelf life response and were in the order Aloe vera > fenugreek > flax seeds. Aluminium pouches showed better stability for all the characteristic properties analysed with least variation upto six months.<#LINE#>Jagganatha Rao P.V.K., Das M. and Das S.K. (2007). Jaggery- a traditional Indian sweetener.@Indian Journal of Traditional Knowledge, 6(1), 95-102.@undefined@Yes$Nerkar Y.S. (2004).@Present scenario and thrust areas for making sugarcane productivity in India.@Financing Agriculture, 29.@Yes$Patil S.D. and Anekar S.V. (2014).@Effect of different parameters and storage conditions on liquid jaggery without adding preservatives.@Inter. J. Res. Eng. Tech, 3(12), 280-283.@Yes$Hunsigi G. (2001).@Sugarcane in agriculture and Industry.@Karnataka Institute of Applied Agricultural Research (KIAAR), Bagalkot.@No$Unde P.A, Adagale P.V., Hashmi S.I. and Raheem Abdul (2011).@Effect of different particle sizes of jaggery powder on storability.@IDOSI Publications, 7(2), 157-160.@Yes$Pattnayak P.K. and Misra M.K. (2004).@Energetic and economics of traditional gur preparation: A case study in Ganjam District of Orissa, India.@Biomass and Bioenergy 26, 79-88.@Yes$Said P.P. and Pradhan R.C. (2013).@Preservation and value addition of jaggery.@Inter J Agri Eng, 6(2), 569-574.@Yes$Fazaeli M., Emam-Djomeh Z., Ashtari A.K. and Omid M. (2012).@Effect of process condition and carrier concentration for improving drying yield and other quality attributes of spray dried black mulberry (Morus nigra) juice.@Inter J Agri Eng, 8(1), 1-20.@Yes$Shrestha A.K., Howes, T., Adhikari, B.P. and Bhandari B.R. (2007).@Water sorption and glass transition properties of spray dried lactose hydrolysed skim milk powder.@LWT-Food Science and Technology, 40(9), 1593-1600.@Yes$Patil J.E., Shinde U.S., Nevkar G.S. and Singh J. (2005).@Clarification Efficiency of Synthetic and Herbal Clarificants in Quality Jaggery Production.@Sugar Tech, 7(2), 77-81.@Yes$Singh H. and Gill P.S. (1954).@Comparative efficiency of certain organic clarificants in gur manufacture.@Second Biannual Conf. Sug. Res and Dev. Workers. 613-615.@No$Joshi K.K. and Pandit S.N. (1959).@Improved technique of gur manufacture and the role of various clarificants.@Indian Journal of Sugar Research and Develpoment, 42-49.@Yes$Laxmikantham (1973).@Importance of jaggery in Andhra Pradesh.@Technology in Sugarcane growing, Andhra Pradesh Agricultural University, Hyderabad (India), 60-70.@No$Agrawal M.D. and Ghosh A.K. (1983).@Effect of cane maturity, nitrogen fertilization, clarificants and additives on keeping qualities of gur.@Indian Sugar, 33, 97-104.@Yes$Baboo B. (1993).@Manufacture of good quality gur and its storage.@Tech. Bull. No.33 IISR Lucknow.@No$Mungare T.S., Jadhav H.D., Patil J.P., Hasure ILR., Jadhav B.S. and Singh Jaswant (2000).@Clarification technique for production of quality jaggery.@Co op. Sugar, 32(4), 283-285.@Yes$Ragavan T., Thirumurugan A., Sathiya K. and Sundra Vadana S. (2011).@A case study: studies on quality jaggery (gur) production with organic clarificants.@Int J For Crop Improv, 2, 207-210.@Yes$Anonymous (1990).@Bureau of Indian Standards.@IS 12923, (Clause 4.3 and 6.1), New Delhi.Chauhan A.K., Sharma S., Sharma P. and Maheshwari R. (2012).@No$Chauhan D.V.S. (1972).@Vegetable production in India.@3rd edn. Ram Prasad and Sons, Agra@No$Shakunthala V.A. and Devraj G. (1991).@Hibiscus abelmoschus L.- a vegetable clarificant in gur making.@National Seminar on Increasing Productivity, Quality and Storability of Jaggrey (gud) at Indian Institute of Sugarcane Research, Lucknow, India.@Yes$Chavan U.D. (2002).@Final report of AD-HOC research project on Preparation of mucilage from okra cortex.@Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri.@Yes$Goula A.M. and Adamopoulos K.G. (2008).@Effect of maltodextrin addition during spray drying of tomato pulp in dehumidified air- ii. Powder properties.@Drying Technology, 26(6), 726-737.@No$Bhandari B.R., Datta N., Crooks R., Rigby S. and Howes T. (1997).@A semi-empirical approach to optimize the quality of drying aids required to spray dry sugar rich foods.@Drying Technology, 15(10), 2509-2525.@Yes$Roos Y. and Karel M. (1991).@Water and molecular weight effects on glass transitions in amorphous carbohydrates and carbohydrate solutions.@Journal of Food Science, 56(6), 1676–1681.@Yes$Singh S., Dubey A., Tiwari L. and Verma A.K. (2009).@Microbial profile of stored jaggery: A traditional Indian sweetener.@Sugar Tech, 11(2), 213-216.@Yes$Kumar D., Singh Jarnail, Rai D.R., Bhatia S. and Kumar Mahesh (2013).@Effect of Modified Atmosphere Packaging on Keeping Quality of Jaggery.@Sugar Tech, 15(2), 203-208.@Yes$Shinde B.N., Marathe A.B., Javalekar D.V. and Kadam S.K. (1983).@Effect of form and colour of polyethylene—the wrapping material on keeping quality of jaggery during storage.@Indian Sugar, 32(12), 937-940.@Yes$Chikkappaiah L., Nayaka H.M.A., Manohar M.P. and Santhosh C. (2017).@Properties of Liquid Jaggery Prepared Using Plant Mucilage As Clarificant.@Int J Recent Sci Res, 8, 19590-19595.@Yes$AOAC. (1990).@Official methods of analysis.@15th edn. Association of official Analytical Chemist. Washinton, DC, USA.@No$Jinapong N., Suphantharika M. and Jamnong P. (2008).@Production of instant soyamilk powder by ultra filtration, spray drying and fluidized bed agglomeration.@J Food Eng, 84(2), 194-205.@No$Goula A.M. and Adamopoulos K.G. (2010).@A new technique for spray drying orange juice concentrate.@Innov Food Sci Emerging Technol, 11(2), 342-351.@Yes$DuBois M., Gilles K.A., Hamilton J.K., Rebers P.A. and Smith F. (1956).@Colorimetric method for determination of sugars and related substances.@Analytical chem., 28(3), 350-356.@Yes$Samborska K., Choromańska A., Witrowa-Rajchert D. and Bakier S. (2011).@Honey spray-drying in a presence of maltodextrin (in Polish).@Postępy Techniki Przetwórstwa Spożywczego, 1, 19-23.@Yes$Mandal D., Tudu S., Mitra S.R. and De G.C. (2006).@Effect of common packaging materials on keeping quality of sugarcane jaggery during monsoon season.@Sugar Techn, 8(2/3), 137-142.@Yes$Chand K., Singh A. and Kulshrestha M. (2012).@Jaggery quality affected by hilly climatic conditions.@Indian Journal of Traditional Knowledge, 11, 172-176.@Yes$Avilla E.L., Rodriguez M.C. and Velasuez H.J.C. (2015).@Influence of maltodextrin and spray drying process condition on sugarcane juice power quality.@Rev. Fac. Nal. Agr. Medellin, 68(1), 7509-7520.@Yes$Barbosa-Canovas G.V., Ortega-Rivas E., Juliano P. and Yan H. (2005).@Food Powders: physical properties, Processing and Functionality.@Kluwer Academic Publisher, New York.@Yes$Kurozawa L.E., Morassi A.G., Vanzo A.A., Park K. J. and Hubinger M.D. (2009).@Influence of spray drying conditions on physicochemical properties of chicken meat powder.@Drying Technology, 27(11), 1248-1257.@No$Geldart D., Harnby N. and Wong A.C. (1984).@Fluidization of cohesive powders.@Powder Technology, 37, 25-37.@Yes$Domian E. and Poszytek K. (2005).@Wheat flour flowability as affected by water activity, storage time and consolidation.@International Agrophysics, 19, 119-124.@Yes$Nayak C.A. and Rastogi N.K. (2010).@Effect of selected additive on microencapsulation of anthocyanin by spray drying.@Drying Technology, 28(12), 1396-1404.@Yes$Ghosal S., Indira T.N. and Bhattacharya S. (2010).@Agglomeration of a model food powder: Effect of maltodextrin and gum Arabic dispersions on flow behavior and compacted mass.@Journal of Food Engineering, 96(2), 222-228.@Yes$Caparino O.A., Tang J., Nindo C.I., Sablani S.S., Power J.R. and Fellman J.K. (2012).@Effect of drying methods on the physical properties and microstructure of mango (Philippine carabao var.) power.@J Food Eng, 111, 135-148.@Yes <#LINE#>Study of Physico-chemical Parameters, Planktonic Diversity and Bacterial Load of Clarias batrachus Cultivation Pond at Bankura, WB, India<#LINE#>Arindam @Ganguly,Asish @Mandal,Mohammad Ali @Khan,Tapas Kumar @Dutta,Subrata @Raha,Pradeep Kumar Das @Mohapatra <#LINE#>23-34<#LINE#>4.ISCA-IRJBS-2017-096.pdf<#LINE#>Department of Microbiology, Vidyasagar University, Midnapore- 721102, West Bengal, India@Department of Botany, Ramananda College, Bishnupur, Bankura-722122, West Bengal, India@Department of Mathematics, Ramananda College, Bishnupur, Bankura-722122, West Bengal, India@Department of Zoology, Bankura Sammilani College, Bankura- 722102, West Bengal, India@Department of Botany, Sidho-Kanho-Birsha University, Purulia-723104, West Bengal, India@Department of Microbiology, Vidyasagar University, Midnapore- 721102, West Bengal, India<#LINE#>14/10/2017<#LINE#>25/11/2017<#LINE#>The exploitation of the natural habitats, uncontrolled introduction of allied exotic fishes and pathogenic infections has brought down the condition of Asian catfish, Clarias batrachus to quite a deplorable state. Currently, a worldwide effort is on the run to cultivate the endangered species in both natural and semi-intensive manner. An extensive study was performed to evaluate the synergistic effect of physico-chemical parameters, planktonic diversity and bacterial flora of C. batrachus cultivation on its productivity. The values of the physicochemical parameters were between 18 to 25ºC for temperature, 6.5 to 7.7 for pH, 3.1 to 4.8 mg/L for dissolved oxygen, 21 to 27 mg/L alkalinity, 70 to 128 mg/L for salinity, 78 to 128 mg/L for total hardness and 176 to 260 mg/L for total dissolved solids. The amount of total ammonia, nitrate and nitrite of the treated pond were 0.02 to 0.06 ppm, 0.1 to 0.4 ppm and 0.1 to 0.5 ppm respectively. Five predominant groups of zooplanktons and five classes of phytoplanktons were identified throughout the study. The viable bacterial counts of pond water and sediment mud were found in low range during winter in comparison to summer season. The bacterial flora consisted mainly of gram negative rods.<#LINE#>FAO. (2014), http://www.fao.org/fishery/countrysector/naso _india/en@undefined@undefined@No$Abalaka S.E. (2013).@Evaluation of the Haematology and Biochemistry of Clarias gariepinus as Biomarkers of Environmental Pollution in Tiga dam, Nigeria.@Braz. Arch. Biol. Technol., 56(3), 371-376.@Yes$Watson S.B. and John L. (2003).@Overview drinking water quality and sustainability.@Water Qual. Res. J. Can., 38(1), 3-13.@Yes$Mikschi E. (1989).@Rotifer distribution in relation to temperature and oxygen content.@Hydrobiol., 186(1), 209-214.@Yes$Pradhan A., Bhaumik P., Das S. and Chaudhuri S.R. (2008).@Phytoplankton Diversity as Indicator of Water Quality for Fish Cultivation.@Am. J. Environ. Sci., 4(4), 406-411.@Yes$Neves I.F., Rocha O., Roche K.F. and Pinto A.A. (2003).@Zooplankton community structure of two marginal lakes of the river Cuba (Mato Grass, Brazil) with analysis of rotifer and Cladocera diversity.@Braz. J. Biol., 63(2), 329-343.@Yes$Majagi S. and kumarVijay K. (2009).@Ecology and abundance of zooplankton in Karanja reservoir.@Environmental monitoring and assessment, 152(1-4), 451.@Yes$Apiratikul R. and Pavasant P. (2008).@Batch and column studies of biosorption of heavy metals by Caulerpa lentillifera.@Bioresour. Technol., 99(8), 2766-2777.@Yes$Roy P.R. and Barat S. (2011).@Influence of water quality on the bacterial contamination of resident loach, Lepidocephalichthys guntea (Hamilton Buchanan) and on a Terai River Lotchka of Darjeeling District, West Bengal, India.@Arch. Environ. Sci., 5, 116-123.@Yes$Muylaert K., Van der Gucht K. and Vloemans N. (2002).@Relationship between bacterial community composition and bottom-up versus top-down variables in four eutrophic shallow lakes.@Appl. Environ. Microbiol., 68, 4740-4750.@Yes$Azam F., Fenchel T., Field J.G., Gray J.S., Meyerr-Reil L.A. and Thingstad F. (1983).@The ecological role of water column microbes in the sea.@Mar. Ecol. Prog. Ser., 10, 257-263.@Yes$Lightner D.V. (1996).@A Hand Book of Shrimp Pathology and Diagnostic Procedures for Disease of Culture Penaeid Shrimp.@World Aquaculture Society, Baton Rouge, Louisiana.@Yes$APHA. (2008).@Standard methods for the examination of water and wastewater.@20th edition (American Public Health Association, Washington, DC).@No$Battish S.K. (1992).@Freshwater Zooplankton of India.@Oxford and IBH publishing Co., New Delhi.@Yes$Al-Deghayem W.A., Al-Balawi H.F., Kandeal S.A. and Suliman E.A.M. (2017).@Gonadosomatic index and some hematological parameters in African catfish Clarias gariepinus (Burchell, 1822) as affected by feed type and temperature level.@Braz. Arch. Biol. Technol., 60, e17160157.@Yes$Ahmad U., Parveen S., Abdel Mola H.R. and Ganai A.H. (2012).@Zooplankton population in relation to physico-chemical parameters of Lal Diggi pond in Aligarh, India.@J. Environ. Biol., 33, 1015-1019.@Yes$Bisht A., Singh U.P. and Pandey N.N. (2012).@Comparative study of seasonal variation in bacterial flora concomitant with farm raised fingerlings of Cyprinus carpio at tarai region of Uttarakhand.@J. Environ. Biol., 35, 363-367.@Yes$Trivedy R.K. (1989).@Limnology of freshwater pond in Mangalore, Oct (National Symposium on Advances in limnology conservation of endangered fish species).@23-25.@Yes$Shiddamallayya N. and Pratima M. (2008).@Impact of domestic sewage on fresh water body.@Indian J. Environ. Biol., 29, 303-308.@No$Schuldiner S., Agmon V., Brandsma J., Cohen A., Friedman E. and Padan E. (1986).@Induction of SOS functions by alkaline intracellular pH in Escherichia coli.@J. Bacteriol., 168(2), 936-939.@Yes$Taglicht D., Padan E., Oppenheim A.B. and Schuldiner S. (1987).@An alkaline shift induces the heat shock response in Escherichia coli.@J. Bacteriol., 169(2), 885-887.@Yes$Maurer L.M., Yohannes E., Bondurant S.S., Radmacher M. and Slonczewski J.L. (2005).@pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12.@J. Bacteriol., 187, 304-319.@Yes$Padan E., Bibi E., Ito M. and Krulwich T.A. (2005).@Alkaline pH Homeostasis in Bacteria: New Insights.@Biochim. Biophys. Acta., 1717(2), 67-88.@Yes$Murhekar G.H. (2011).@Determination of Physico-Chemical parameters of Surface Water Samples in and around Akot City.@Res. J. Chem. Sci., 1(2), 183-187.@Yes$Bhatnagar A. and Garg S.K. (2000).@Causative factors of fish mortality in still water fish ponds under sub-tropical conditions.@Aquaculture., 1(2), 91-96.@Yes$Venkatesh K.R., Rajendran M. and Murugappan A. (2009).@A correlation study on physico-chemical characteristics of domestic sewage.@Nat. Environ. Pollut. Tech., 8, 141-145.@No$Shashi and Dwivedi A.K. (2009).@Numerical interdependence in pH, acidity and alkalinity of polluted river water.@J. Environ. Biol., 30(5), 773-775.@Yes$Yusoff F.M., Banerjee S., Khatoon H. and Shariff M. (2011).@Biological Approaches in Management of Nitrogenous Compounds in Aquaculture Systems.@Dyn. Biochem. Process Biotechnol. Mol. Biol., 5(1), 21-31.@Yes$Dutta T.K. and Patra B.C. (2013).@Biodiversity and seasonal abundance of Zooplankton and its relation to physico-chemical parameters of Jamunabundh, Bishnupur, India.@Int. J. Sci. Res., 3, 76-82.@Yes$Ratushnyak A.A., Borisovich M.G., Vallev V.S., Ivanov D.V., Andreeva M.G. and Trushin M.V. (2006).@The hydrochemical and hydrobiological analysis of the condition of the kuibyshev reservoir littorals (Republic of Tatarstan, Russia).@Ekoloji., 15(61), 22-28.@Yes$Weber-Scannell P.K. and Duffy L.K. (2007).@Effects of Total Dissolved Solids on Aquatic Organisms: A Review of Literature and Recommendation for Salmonid Species.@Am. J. Environ. Sci., 3(1), 1-6.@Yes$Ayyappan S. (2010).@Handbook of Fisheries and Aquaculture.@ICAR, New Delhi.@Yes$Pillay T.V.R. (2005).@Aquaculture: Principles and Practices.@Blackwell Science Ltd. Oxford, London;@No$Courtenay W.R., Sahlman H.F., Miley W.W. and Herrema D.J. (1974).@Exotic fishes in fresh and brackish waters of Florida.@Biol. Conserv., 6(4), 292-302.@Yes$Knud-Hansen C.F., Batterson T.R., McNabb C.D., Hadiroseyani Y., Dana D. and Eidman H.M. (1990).@Hatchery techniques for egg and fry production of Clarias batrachus (Linnaeus).@Aquaculture., 89, 9-19.@Yes$Tucker C.S., Lloyd S.W. and Busch R.L. (1984).@Relationship between un-ionized ammonia in channel catfish ponds.@Hydrobiol., 111, 75-79.@Yes$Dawah A.M. and Gomaah S.A. (2005).@Phytoplankton dynamics in earthen ponds stocked with African catfish (Clarias gariepinus) feeding with different diets.@Egyptian J. Phycol., 6, 1-15.@Yes$Chowdhury M.B.R., Uddin M.N. and Begum A. (1994).@Bacterial flora in catfish, Clarias batrachus pond.@Bangladesh J. Aquacult., 16, 39-42.@Yes @Short Communication <#LINE#>Quantification of bioelements in seed and oil of black chia (Salvia hispanica L)<#LINE#>Alejandro Alonso-Calderón@Calderón,Juana DeisySantamaría-@Juárez,Jorge R. Omaire-Vargas@Vargas,Esmeralda Vidal-Robles@Robles,Jenaro LeocadioVarela-Caselis@Caselis,D. @Cruz,Edith Chávez-@Bravo <#LINE#>35-39<#LINE#>5.ISCA-IRJBS-2017-093.pdf<#LINE#>Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico@Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico@Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico@Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico@Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico@Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico@CICM del Instituto de Ciencias de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla<#LINE#>6/10/2017<#LINE#>28/11/2017<#LINE#>The Aztec empire stood out from a nutritional point of view by having corn, beans, amaranth and chia, as main components of their diet. Chia had also medical applications such as in infusions for gastrointestinal problems, and respiratory, ophthalmology, obstetrics, skin treatments among others. This research was conducted to determine the concentration of the metals Ni, Zn, Mn, Fe, Cd, Cu, Ca, K and Na, using a Perkin-Elmer Precisely, Model 400 AAanalyst spectrophotometer, equipped with a flame atomization system. The results obtained for the chia seed were: nickel: 1.407 ppm, iron: 338.125 ppm, manganese: 6.937 ppm, zinc: 6.3 ppm, copper: 4.375, boron: 6.316 ppm, cadmium 0.0, calcium: 228 ppm, potassium: 24.743 ppm y sodium: 3.089 ppm. Calcium is essential for all organisms as it is a structural component of cell walls and bones, for the metabolism of bone fixation it is needed synergy with nickel, boron, zinc, manganese and copper, Iron deficiency is the most prevalent nutritional deficiency in the world and Mexico is no exception causing anemia, being chia an excellent source of these bioelements.<#LINE#>Cahill J.P. (2003).@Ethnobotany of chia, Salvia hispanica L. (Lamiaceae).@Economic Botany, 57(4), 604-618.@Yes$Ayerza R. (2009).@The Seed´s Protein and Oil Content, Fatty Acid Composition, and Growing Cycle Length of a Single Genotype of chia (Salvia hispanica L.) as Affected by Environmental Factors.@J. Oleo Sci., 58(7), 347-354.@Yes$Ixtaina V.Y., Vega A., Nolasco S.M., Tomás M.C., Gimeno M., Bárzana E. and Tecante A. (2010).@Supercritical carbon dioxide extraction of oil from Mexican chia seed (Salvia hispanica L.): Characterization and process optimization.@J Supercrit Fluids, 55, 192-199. https://doi.org/10.1016/j.supflu.2010.06.003@Yes$Ixtaina V.Y., Martínez M.L., Spotorno V., Mateo C.M., Maestri D.M., Diehl B.W.K., Nolasco S.M., Tomás M. C. (2011).@Characterization of chia seed oils obtained by pressing and solvent extraction.@Journal of Food Composition and Analysis, 24(2), 166-174.@Yes$Kang J.X. (2005).@Balance of Omega–6/Omega–3 Essential Fatty Acids Is Important for Health.@Simopoulos AP (ed): Nutrition and Fitness: Mental Health, Aging, and the Implementation of a Healthy Diet and Physical Activity Lifestyle, World Rev Nutr Diet. Basel, Karger, 95, 93-102.@Yes$Poudyal H., Panchal S.K., Waanders J., Ward L. and Brown L. (2012).@Lipid redistribution by α-linolenic acid-rich chia seed inhibits stearoyl-CoA desaturase-1 and induces cardiac and hepatic protection in diet-induced obese rats.@J Nutr Biochem, 23(2), 153-162. https://doi.org/10.1016/j. jnutbio.2010.11.011@Yes$Castro M.R., Pratt D.E. and Miller E.E. (1986).@Natural antioxidants of chia seeds.@Proceedings of The World Conference on Emerging Technologies in the Fats and Oils Industry edited and published by American Oil Chemists Society, Champaign, Illinois, USA, 392-396.@Yes$Taga M.S., Miller E.E. and Pratt D.E. (1984).@Chia seeds as a source of natural lipid antioxidants.@Journal of American Oil Chemists Society, 61(5), 928-931.@Yes$Imran M., Nadeem M., Manzoor M.F., Javed A., Ali Z., Akhtar M.N, Ali M. and Hussain Y. (2016).@Fatty acids characterization, oxidative perspectives and consumer acceptability of oil extracted from pre-treated chia (Salvia hispanica L.) seeds.@Lipids Health Dis., 15(1), 162. https://doi.org/10.1186/s12944-016-0329-x@Yes$Ayerza R. and Coates W. (2000).@Dietary Levels of Chia: Influence on Yolk Cholesterol, Lipid Content and Fatty Acid Composition for Two Strains of Hens.@Poult. Sci.,79(5), 724-739.@Yes$Vuksan V., Whitham D., Sievenpiper J.L., Jenkins A.L., Rogovik A.L., Bazinet R.P., Vidgen E. and Hanna A. (2007).@Supplementation of Conventional Therapy with the Novel Grain Salba (Salvia hispanica L.) Improves Major and Emerging Cardiovascular Risk Factors in Type 2 Diabetes.@Diabetes Care, 30(11), 2804-2810. https://doi.org/10.2337/dc07-1144@Yes$Alonso C.A., Chávez B.E., Rivera A., Montalvo P.C., Arroyo T.R., Monterrosas S.M., Jiménez S.T. and Tapia H.A. (2013).@Characterization of Black Chia Seed (Salvia hispanica L) and Oil and Quantification of β-sitosterol.@International Research Journal of Biological Sciences, 2(1), 70-72.@Yes$Ratner R. and Ortiz M. (2008).@Fitoesteroles: Una alternativa natural al tratamiento de la hipercolesterolemia.@Revista Obesidad, 5(1), 24-29.@No$Fierro A., Vásquez Y., Reyes P. and Boza S. (2004).@Determinación Cuantitativa del Sitosterol presente en vegetales de la dieta, Posibles implicaciones para su uso preventivo en poblaciones susceptibles.@Clínica Ciencia, 2(2), 43-48.@Yes$Bushway A.A., Belyea P.R. and Bushway R.J. (1981).@Chia seed as a source of oil, polysaccharide, and protein.@Journal of Food Science, 46(5), 1349-1350.@Yes$Scientific Opinion (2009).@Scientific Opinion of the Panel on Dietetic Products Nutrition and Allergies on a request from the European Commission on the safety of ‘Chia seed (Salvia hispanica) and ground whole Chia seed’ as a food ingredient.@The EFSA Journal, 996, 1-26. https://doi.org/10.2903/j.efsa.2009.996@No$Huheey E.J. (1981).@Química Inorgánica.@Ed. Harla, Segunda Edición., 805-827.@No$Anke M., Groppel B., Kronemann H. and Grün M. (1984).@Nickel--an essential element.@IARC Sci Publ, 53, 339-365.@No$Vielma J.R., Mora Mora M., Marino Alarcón O., Hernández G., Linares L.J., Urdaneta Romero H. and Arévalo González E. (2012).@Estudio comparativo de la excreción urinaria de boro, calcio, magnesio y fósforo en mujeres posmenopáusicas con y sin osteoporosis.@Invest Clin, 53(1), 3-15.@Yes$Nielsen Forrest H. (1994).@Biochemical and Physiologic Consequences of Boron Deprivation in Humans.@Environmental Health Perspectives Environ Health Perspect, 102(Suppl 7), 59-63.@Yes$Brambila E y Luna A. (2012).@Metabolismo del zinc y las metalotioneinas en los procesos quirúrgicos.@Mensaje Bioquímico, XXXVI, 39-50.@Yes$Martín M.I., del Portal J.I. and del Portal J.C.I. (2000).@Vitaminas y minerales.@Editorial Complutense.@Yes$Landete-Castillejos T., Molina-Quilez I., Estevez J.A., Ceacero J.A., Garcia A.J. and Gallego L. (2012).@Alternative hypothesis for the origin of osteoporosis: the role of Mn.@Frontiers in Bioscience E, 4, 1385-1390.@Yes$Larsen Hans R. (2010).@Copper: The Missing Link?.@The AFIB REPORT.@Yes @Review Paper <#LINE#>Gene transfer in eukaryotic cells: current applications and implications<#LINE#>Bruh @Asmelash,Haftom @Brhane <#LINE#>40-44<#LINE#>6.ISCA-IRJBS-2017-055.pdf<#LINE#>College of Agri. and Environmental Sciences, School of Animal and Range Sciences, Post Box: 138, Haramaya University, Dire Dawa, Ethiopia@College of Natural and Computational Science; Department of Biology, Haramaya University, Ethiopia<#LINE#>16/6/2017<#LINE#>14/11/2017<#LINE#>Gene transfer is indispensable to modify the genome of organisms and enhance their overall performance as compared to the average performance of their ancestors. Gene transfer in eukaryotic cells gives way to decipher the genotypes responsible for different traits, agricultural enhancement and therapeutic strategy. The three commonly used gene transfer methods are physical, chemical and biological based ones. It is reported that the application of transgenenesis in livestock agriculture comprises of increment of growth rate, milk production and composition, feed usage and carcass composition, disease resistance, enhanced reproductive and prolificacy enhancement. Now days, liposome mediated gene transfer is believed to be the most promising widely used gene transfer method in eukaryotic cells. In principle, the DNA fragments encapsulated with liposomes adheres to the cell membranes which in turn will fuse with them to transfer genes or DNA fragments of interest. Thus, the DNA fragment of interest enters into the nucleus of a cell that will later undergo reproduction. In order to achieve maximum transfection efficiency in eukaryotic cells, it is essential to optimize several parameters including lipid to DNA charge ratio and DNA amount concentration. Liposome mediated gene transfer is relatively easy, direct, inexpensive, reproducible and efficient.<#LINE#>Verma I. and Somia N. (1997).@Gene therapy: promises, problems and prospects.@Nature, 389, 239-242.@Yes$Tae K. and James H. (2010).@Mammalian cell transfection: the present and the future.@Anal Bioanal Chem, 397(8), 3173-3178.@Yes$Wurm F. (2004).@Production of recombinant protein therapeutics in cultivated mammalian cells.@Nat Biotechnol, 22(11), 1393-1398.@Yes$Patil S., Rhodes D. and Burgess D.J. (2005).@Biophysical characterization of anionic lipoplexes.@BiochemicalEtBiophysicalActa, 1711, 1-11.@Yes$Karmali P. and Chaudhuri A. (2007).@Cationic liposomes as non-viral carriers of gene medicines: Resolved issues, open questions, and future promises.@Medicinal Research Reviews, 27(5), 696-722.@Yes$Thierry A. and Dritschilo A. (1992).@Intracellular availability of unmodified, phosphorothioates and liposomal encapsulated oligodeoxynucleotides for antisense activity.@Nucleic Acids Res, 20(21), 5691-5698.@Yes$Amax A. (2007).@Collaboration to Develop High-throughput Devices for Primary Cell Transfection.@@No$Goodman R.M., Hauptli H., Crossway A. and Knauf V.C. (1987).@Gene transfer in crop improvement.@Science, 236(4797), 48-54.@Yes$Wheeler M.B. (2007).@Agricultural applications for transgenic livestock.@Trends in Biotechnology, 25(5), 204-210.@Yes$Uzogara S. (2000).@The impact of genetic modification of human foods in the 21st century: a review.@Biotechnol Adv, 18(3), 179-206.@Yes$Lorence A. and Verpoorte R. (2004).@Gene transfer and expression in plants.@Methods Mol Biol, 267, 329-350.@Yes$Robl J.M., Wang Z., Kasinathan P. and Kuroiwa Y. (2006).@Transgenic animal production and animal biotechnology.@Theriogenology, 67, 127-133.@Yes$Whitelaw C., King T. and Lilico S. (2008).@Production of transgenic farm animals by viral vector-mediated gene transfer.@Reprod.Domest. Anim., 43(S2), 355-358.@Yes$Brophy B., Smolenski G., Wheeler T., Wells D., L@Cloned transgenic cattle produce milk with higher levels of β-casein and κ-casein.@Nature biotechnology, 21(2), 157-162.@Yes$Bremel R.D., Yom H.C. and Bleck G.T. (1989).@Alteration of milk composition using molecular genetics.@Journal of dairy science, 72(10), 2826-2833.@Yes$Rothschild M.F., Jacobson C., Vaske D.A., Tuggle C.K., Short T.H., Sasaki S. and McLaren D.G. (1994).@A major gene for litter size in pigs.@Proceedings of the Fifth World Congress Genet. Applied to Livestock Production, 21, 225-228.@Yes$Osanto S., Brouwenstyn N., Vaessen N., Figdor C.G., Melief C.J.M. and Schrier P.I. (1993).@Immunization with Interleukin-2 Transfected Melanoma Cells. A Phase I–II Study in Patients with Metastatic Melanoma.@University Hospital Leiden. Human gene therapy, 4, 323-330.@Yes$Arienti F., Sulé-Suso J., Belli F., Mascheroni L., Rivoltini L., Melani C. and Parmiani G. (1996).@Limited antitumor T cell response in melanoma patients vaccinated with interleukin-2 gene-transduced allogeneic melanoma cells.@Human gene therapy, 7(16), 1955-1963.@Yes$Nabel G.J., Gordon D., Bishop D.K., Nickoloff B.J., Yang Z.Y., Aruga A. and Chang A.E. (1996).@Immune response in human melanoma after transfer of an allogeneic class I major histocompatibility complex gene with DNA–liposome complexes.@Proceedings of the National Academy of Sciences, 93(26), 15388-15393.@Yes$Deshane J., Siegal G.P., Wang M., Wright M., Bucy R.P., Alvarez R.D. and Curiel D.T. (1997).@Transductional efficacy and safety of an intraperitoneally delivered adenovirus encoding an anti-erbB-2 intracellular single-chain antibody for ovarian cancer gene therapy.@Gynecologic oncology, 64(3), 378-385.@Yes$Stopeck A.T., Hersh E.M., Akporiaye E.T., Harris D.T., Grogan T., Unger E. and Stahl S. (1997).@Phase I study of direct gene transfer of an allogeneic histocompatibility antigen, HLA-B7, in patients with metastatic melanoma.@Journal of Clinical Oncology, 15(1), 341-349.@Yes$Kun L.E., Gajjar A., Muhlbauer M., Heideman R.L., Sanford R., Brenner M. and Facchini S. (1995).@Stereotactic injection of herpes simplex thymidine kinase vector producer cells (PA 317- G1Tk1SvNa.7) and intravenous ganciclovir for the treatment of progressive or recurrent primary supratentorial pediatric malignant brain tumors.@Hum Gene Ther, 6(9), 1231-1255.@Yes$Haynes B. (1996).@HIV vaccines: where we are and where we are going.@Lancet, 348, 933-937.@Yes$Weber J. (1997).@Distinguishing between response to HIV vaccine and response to HIV.@Lancet, 350, 230-231.@Yes$Woolf T. (1998).@Therapeutic repair of mutated nucleic acid sequences.@Nature Biotechnol, 16(4), 341-344.@Yes$Feng M., Jackson W.H., Goldman C.K., Rancourt C., Wang M., Dusing S.K. and Curiel D.T. (1997).@Stable in vivo gene transduction via a novel adenoviral/retroviral chimeric vector.@Nat Biotech, 15, 866-870.@Yes$Anthony D., Pan Y., Wu S., Shen F. and Guo Y. (1998).@Ex vivo and in vivo IGF-I antisense RNA strategies for treatment of cancer in humans.@Adv. Exp. Med. Biol., 451, 27-34. [PMC free article: PMC28332] [PubMed: 9391052]@Yes$Blaese R., Culver K. and Anderson W. (1990).@The ADA human gene therapy clinical protocol.@Hum Gene Ther, 1, 327-362.@Yes$Rosenberg S.A., Aebersold P., Cornetta K., Kasid A., Morgan R.A., Moen R. and Merino M.J. (1990).@Gene transfer into humans: immunotherapy of patients with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction.@N Engl J Med., 323, 570-578.@Yes$Mahvi D.M., Sondel P.M., Yang N.S., Albertini M.R., Schiller J.H., Hank J. and Logrono R. (1997).@Phase I/IB Study of Immunization with Autologous Tumor Cells Transfected with the GM-CSF Gene by Particle-Mediated Transfer in Patients with Melanoma or Sarcoma University of Wisconsin, Madison, Wisconsin.@Human gene therapy, 8(7), 875-891.@Yes$Nguyen D.M., Spitz F.R., Yen N., Cristiano R.J. and Roth J.A. (1996).@Gene therapy for lung cancer: enhancement of tumor suppression by a combination of sequential systemic cisplatin and adenovirus-mediated p53 gene transfer.@The Journal of thoracic and cardiovascular surgery, 112(5), 1372-1377.@Yes$Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M. and Danielsen M. (1987).@Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.@Proceedings of the National Academy of Sciences, 84(21), 7413-7417.@Yes$Pleyer U. and Dannowski H. (2002).@Delivery of genes via liposomes to corneal endothelial cells.@Drug News Perspect, 15(5), 283-289.@Yes$Huang L. and Li S. (1997).@Liposomal gene delivery: A complex package.@Nat.Biotechnol., 15, 620-621.@Yes$Hug P. and Sleight R.G. (1991).@Liposomes for the transformation of eukaryotic cells.@BiochimBiophys Acta, 1097(1), 1-17.@Yes$Zhu N., Liggitt D., Liu Y. and Debs R. (1993).@Systemic gene expression after intravenous DNA delivery into adult mice.@Science, 26, 209-211.@Yes$Metselaar J.M., Bruin P., de Boer L.W., de Vringer T., Snel C., Oussoren C. and Hennink W.E. (2003).@A novel family of L-amino acid-based biodegradable polymer− lipid conjugates for the development of long-circulating liposomes with effective drug-targeting capacity.@Bioconjugate chemistry, 14(6), 1156-1164.@Yes$Kim J.K., Choi S.H., Kim C.O., Park J.S., Ahn W.S. and Kim C.K. (2003).@Enhancement of polyethylene glycol (PEG)‐modified cationic liposome‐mediated gene deliveries: effects on serum stability and transfection efficiency.@Journal of pharmacy and pharmacology, 55(4), 453-460.@Yes$Immordino M.L., Dosio F. and Cattel L. (2006).@Stealth liposomes: review of the basic science, rationale, and clinical applications, existing and potential.@International journal of nanomedicine, 1(3), 297-315.@Yes$Brem G., Brenig B., Kräusslich H., Müller M., Springmann K. and Winnacker E. (1988).@Gene transfer by DNA microinjection of growth hormone genes in pigs.@Eleventh International Congress on Animal Reproduction and Artificial Insemination. Dublin.@Yes$Liu Z., Cashion L.M. and Twu J.J. (1997).@A systematic comparison of relative promoter/enhancer activities in mammalian cell lines.@Analytical biochemistry, 246(1), 150-152.@Yes$Fedele M., Pentimalli F., Baldassarre G., Battista S., Klein-Szanto A.J., Kenyon L. and Viglietto G. (2005).@Transgenic mice overexpressing the wild-type form of the HMGA1 gene develop mixed growth hormone/prolactin cell pituitary adenomas and natural killer cell lymphomas.@Oncogene, 24(21), 3427-3435.@Yes$Stallwood Y., Briend E., Ray K.M., Ward G.A., Smith B.J., Nye E. and McKenzie G.J. (2006).@Small interfering RNA-mediated knockdown of notch ligands in primary CD4+ T cells and dendritic cells enhances cytokine production.@The Journal of Immunology, 177(2), 885-895.@Yes