@Research Paper <#LINE#>Contribution of phosphate solubilizing activity to plants by Pseudomonas sp. having antifungal activity<#LINE#>Latt@Zaw Ko, Zaw Oo@Win, Yu@SanSan,Kyaw@Ei Phyu,Lynn@Tin Mar <#LINE#>1-7<#LINE#>1.ISCA-IRJBS-2017-072.pdf<#LINE#>Biotechnology Research Department, Ministry of Education, Myanmar@Biotechnology Research Department, Ministry of Education, Myanmar@Biotechnology Research Department, Ministry of Education, Myanmar@Biotechnology Research Department, Ministry of Education, Myanmar@Biotechnology Research Department, Ministry of Education, Myanmar<#LINE#>1/8/2017<#LINE#>2/10/2017<#LINE#>The application of chemical fungicides has caused health hazards in animals and humans due to their residual toxicity. The objective of this research work is to find antagonistic bacteria having plant growth promoting activity for the control of some plant pathogenic fungi. Isolation of antagonistic bacteria from rhizosphere of rice, colonial and microscopic morphology and biochemical characterization were done. For antagonistic activity, dual culture and well diffusion method were employed. P-solubilizing activity was detected by plate screening and Vogel method. Isolated bacteria were assumed as Pseudomonas sp. according to its characteristic. This bacteria exhibited antagonistic activity against eight plant pathogenic fungi. The highest activity gave against fungi infected on green gram (26 mm) by dual culture method and against Fusarium sp. in giving 21 mm of inhibition zone by well diffusion method. 264.20 ppm and 235.77 ppm of soluble phosphate was detected by this isolate. Isolated bacteria exhibited antagonistic activity against eight plant pathogenic fungi by dual culture and well diffusion methods. Its activity was higher on PDA media than on nutrient media. In addition, isolated bacteria also possess P-solubilizing activity.<#LINE#>Spadaro D. and Gullino M.L. (2004).@State of the art and future prospects of the biological control of postharvest fruit diseases.@Int J Food Microbio, 91(2), 185-194.@Yes$Whipps J.M. (2001).@Microbial interactions and biocontrol in the rhizosphere.@Jou of Experi Bot, 52, 487-511.@Yes$Dev Neha and Dawande A.Y. (2010).@Biocontrol of soil borne plant pathogen Rhiozoctonia solani using Trichoderma spp. and Pseudomonas fluorescens.@Asiatic J Biotech Res., 01, 39-44.@Yes$Holmes G.J. and Eckert J.W. (1999).@Sensitivity of Penicillium digitatum and P.italicum to postharvest citrus fungicides in California.@Phytopatho, 89, 716-721.@Yes$Kinay P., Mansour M.F., Gabler F.M., Margosan D.A. and Smilanick J.L. (2007).@Characterization of fungicide-resistant isolates of Penicillium digitatum collected in California.@Crop Prot, 26(4), 647-656.@Yes$Deacon J.W. (1991).@Significance of ecology in the development of biocontrol agents against soil-borneplant pathogens.@J of Biocon Sci and Tech, 1(1), 5-20.@Yes$Compant S., Duffy B., Nowak J., Clément C. and Barka E.A. (2005).@Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects.@Appli and Envi Micro., 71(9), 4951- 4959.@Yes$Fernando W.G.D., Ramarathnam R., Krishnamoorthy A.S. and Savchuk S.C. (2005).@Identification and use of potential bacterial organic antifungal volatiles in biocontrol.@Soil Biol Biochem., 37, 955-964.@Yes$Whipps J.M. (1997).@Development in the biological control of soil-borne plant pathogens.@Advan in Bota Res., 26, 1-134.@Yes$Ryder M.H., Stephens P.M. and Bowen G.D. (1994).@Improving plant productivity with rhizosphere bacteria.@In: Glen Osmond, SA: CSIRO Division of Soils, refs, illus, index, 288.@Yes$Srivastava R. and Shalini (2008).@Antifungal activity of Pseudomonas fluorescens against different plant pathogenic fungi.@The Inter J of Micro., 7(2), 2789-2796.@Yes$Sivan A. and Chet I. (1989).@Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum.@J of Gen Micro, 135, 675-682.@Yes$McKeen C.D., Reilly C.C. and Pusey P.L. (1986).@Production and partial characterization of antifungal substances antagonistic to Monilinia fructicola from Bacillus subtilis.@Phytopatho, 76, 136-139.@Yes$Chet I. (1988).@Mycoparasitism and lytic enzymes.@153-171. In G. E. Harman and C. P. Kubicek (ed.), Trichoderma and Gliocladium, 2. Enzymes, biological control and commercial appli- cation. Taylor and Francis Ltd., London, United Kingdom.@Yes$Fiddaman P.J. and Rossall S. (1993).@The production of antifungal volatiles by Bacillus subtilis.@J of Appli Bacterio, 74(2), 119-126.@Yes$Chernin L. and Chet I. (2002).@Microbial enzymes in biocontrol of plant pathogens and pests.@Enzymes in the Environment: Activity, Ecology, and Applications. (R Burns and R Dick eds.), Marcel Dekker, Inc., New York, 171-225.@Yes$Serra Mauro Dalla, Menestrina Gianfranco, Carpaneto Armando, Gambale Franco, Fogliano Vincenzo and Ballio Alessandro (2003).@Molecular mechanisms of action of syringopeptins, antifungal peptides from Pseudomonas syringae pv. Syringae.@CRC Press 203; 272-295. Print ISBN: 978-0-415-29852-0, eBook ISBN: 978-0-203-98664-6. https://doi.org/10.1201/9780203986646.ch13.@Yes$Raaijmakers J.M., Vlami M. and Souza J.T. (2002).@Antibiotic production by bacterial biocontrol agents.@Antonie Van Leeuwenhoek, 81, 537-547.@Yes$Vogel A.I. (1968).@A text of quantitative analysis including elementary instrumental analysis.@The English Lan Book soci & Long-mans, 3rd Ed: 180.@Yes$Cartwright D.K., Chilton W.S. and Benson D.M. (1995).@Pyrrolnitrin and phenazine production by Pseudomonas cepacia, strain 5.5 B, a biological agent of Rhizoctonia solani.@Appl Microbiol Biotech, 43(2), 211-116.@Yes$Rosales A.M., Thomashow L., Cook R.J. and Mew T.W. (1995).@Isolation and identification of antifungal metabolites produced by rice-associated antagonistic Pseudomonas spp.@Phytopatho, 85(9), 1028-1032.@Yes$Winkelmann G. and Drechsel H. (1997).@Microbial siderophores. Biotechnology. HJ Rehm and G. Reed (eds.).@Second Edition. VCH, Weinheim, 7, 199-246.@Yes$O’Sullivan D.J. and O’Gara F. (1992).@Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens.@Microbiol Rev., 56(4), 662-676.@Yes$Anjaiah V., Koedam N., Nowak-Thompson B., Loper J.E., Hofte M., Tambong J.T. and Cornelis P. (1998).@Involvement of phenazines and anthranilate in the antagonism of Pseudomonas aeruginosa PNA1 and Tn5 derivatives toward Fusarium spp. and Pythium spp. Mol Pla-Micro Inter.@11(9), 847-854.@Yes$Vanitha S. and Samiyappan R. (2007).@Screening of bacterial antagonistic microorganisms under in vitro conditions against Alternaria chlamydospora causing leaf blight disease in Solanum nigrum L.@Biomed., 2(2), 155-163.@Yes$Adhikari A., Dutta S., Nandi S., Bhattacharya I., Roy M., de Sarkar G. and Mandal T. (2013).@Antagonistic potentiality of native rhizobacterial isolates against root rot disease of okra, incited by Rhizoctonia solani.@Afri J of Agri Res., 8(4), 405-412.@Yes$Crowe Jonathan D. and Olsson Stefan (2001).@Induction of laccase activity in Rhizoctonia solani by antagonistic Pseudomonos fluorescens strains and a range of chemical treatments.@Appl Environ Microbiol, 67(5), 2088-2094.@Yes$Gupta C.P., Sharma A., Dubey R.C. and Maheshwari D.K. (1999).@Pseudomonas aeuriginosa as a strong a strong anatagonis of Macrophomina phaseolina and Fusarium oxyporum.@Cytobios, 99, 183-189.@Yes$Gupta C.P., Bhavesh K., Dubey R.C., Maheshwari D.K. (2006).@Chitinase mediated destructive antagonistic potential of Pseudomonas aeruginosa GRC1 against Sclerotinia scleotiorum causing stem rot peanut.@Bio Control, 51, 821-835. doi:10.1111/j.1365-2672.2004. 02252.x. J Appl Microbiol, 96, 1151-1160.@Yes$Zehnder G., Murphy J.F., Sikora E.J. and Kloepper J.W. (2001).@Application of rhizobacteria for induced resistance.@Eur J of Plant Patho, 107, 39-50.@Yes @Short Communication <#LINE#>DNA barcoding for identification of Conocephalus dorsalis (Orthoptera: Tettigoniidae) from Northern Kerala using Cytochrome Oxidase Subunit I Gene<#LINE#>V.C.@Muhammedali,V.P.@Akhilesh,C.D.@Sebastian <#LINE#>8-10<#LINE#>2.ISCA-IRJBS-2017-074.pdf<#LINE#>Department of Biotechnology, SAS SNDP Yogam College Konni, Pathanamthitta, Kerala 689 691, India@Molecular Biology Laboratory, Department of Zoology, University of Calicut, Kerala 673 635, India@Molecular Biology Laboratory, Department of Zoology, University of Calicut, Kerala 673 635, India<#LINE#>8/8/2017<#LINE#>5/10/2017<#LINE#>Grasshoppers are widely distributed in all ecosystems. Tettigoniidae is a family of grasshoppers including katydids or bush crickets in the order Orthoptera. Approximately 6,400 species of Tettigoniids are found around the world. Conocephalus dorsalis, Tettigoniidae family, was collected from rice fields of Northern Kerala. It is an omnivorous insect that feeds grasses, seeds and small insects including pests. They are predators on rice field, controlling pest populations. The present study deals with species identification and revealed phylogenetic history of C. dorsalis using cytochrome oxidase subunit I (COI) gene encoded as mitochondria. The COI gene of C. dorsalis, 589bp are sequenced and obtained was deposited in the NCBI GenBank.<#LINE#>Thakkar Bhumi, Parmar Suzen and Parikh Pragna (2015).@Study on Diversity of Orthoptera fauna in South Gujarat, India.@International Journal of Pure and Applied Zoology, 3(4), 368-374.@Yes$Mahasneh A. and Katbeh Barder A. (2004).@A taxonomic study on the long horned grasshoppers of Jordan (Orthoptera: Tettigoniidae).@Biologiezentrum, 2, 245-264.@Yes$Miskelly James (2013).@Firth North American records of Conocephalus dorsalis (Latereille 1804) (Orthoptera: Tettigoniidae).@The Pan Pacific Entomologist, 89(1), 69-71.@Yes$Helda Orbani Rosa and Mariana (2014).@Predators and Parasitoids on Ricefields of Back Swamp and Tidal Swamp Lands in South Kalimantan.@International Journal of Science and Research, 3(10), 759-763.@No$Hebert Paul D.N. and Gregory Ryan T. (2005).@The promise of DNA Barcoding for Taxonomy.@Systematic Biology, 54(5), 852-859.@Yes$McCleanaghan Beverly, Gibson Joel F., Shokralla Shadi and Hajibabaei Mehrad (2015).@Discrimination of grasshopper (Orthoptera: Acrididae) diet and nich overlap using next generation sequencing of gut contents.@Ecology and Evolution, 5(15), 3046-3055.@Yes$Mashhoor K., Akhilesh V.P., Sebastian C.D., Rosy P.A. and Kottickal L.V. (2012).@Molecular Phylogenetic Status of Microcentrum rhombifolium in the Family Tettigoniidae.@Developmental Microbiology and Molecular Biology, 3, 9-15.@Yes$Jisha Krishnan E.K. and Sebastian C.D. (2015).@Species Authentication and Taxonomic Relationship Assessment of Ceriagrion coromandelianum (Fabricus) (Zygoptera: Coenogrianidae) using the Molecular Marker Cytochrome Oxidase I gene.@International Journal of Current Research, 7(12), 23997-23999.@No$Ruksana K. and Sebastian C.D. (2015).@Genetic Diversity of the Cigratte Beetle Lasioderma serricorne (Fabricus) Derived from Mithochondrial DNA Sequence.@International Journal of Pharma and Bioscience, 6(3), 877-882.@Yes$Sreejith K. and Sebastian C.D. (2015).@Molecular Phylogeny of Thaia subrufa Based on the Mitochondrial Cytochrome Oxidase Subunit I (COI) gene.@Journal of Entomology and Zoology Studies, 3(3), 135-139.@Yes$Akhilesh V.P. and Sebastian C.D. (2014).@Molecular barcoding and Phylogeny analysis of Herpetogramma stulasis (Lepidoptera: Crambidae) using COI gene sequence.@International Journal of Advanced Life Science, 7(3), 463-466.@Yes$Sanger F. and Coulson A.R. (1975).@A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.@Journal of Molecular Biology, 94(3), 441-448.@Yes$Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S. (2013).@MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0.@Molecular Biology and Evolution, 30(12),2725-2729.@Yes @Review Paper <#LINE#>Diversity and checklist of tree species in Thattekkad Bird Sanctuary, Kerala, India<#LINE#>M.P.@Rijuraj,M.D.@Joseph,R. @Sugathan <#LINE#>11-22<#LINE#>3.ISCA-IRJBS-2017-079.pdf<#LINE#>Indian Institute of Information Technology and Management- Kerala (IIITM-K) Thiruvananthapuram, Kerala, India@Indian Institute of Information Technology and Management- Kerala (IIITM-K) Thiruvananthapuram, Kerala, India@Thattekkad Bird Sanctuary, Kerala Forest Department (KFD), Thattekad, Kerala, India<#LINE#>14/8/2017<#LINE#>7/10/2017<#LINE#>The present study is a unique attempt to know more about the tree species in Thattekkadu bird sanctuary. Trees are the dominant plant group of Thattekkad bird sanctuary. To analysis the tree species, we prepare checklist of tree species and to study the diversity we calculated richness at level of genus and species. Our study reveals good results. This sanctuary contains 163 tree species which belong to 111 genera and 48 families. Family with maximum number of tree species includes Fabaceae with 16 tree species. 27.60 % tree species are endemics and considerable percentages of tree species are needed to be conserved. The finding of the study suggests that the forest of the sanctuary stands undisturbed and healthy. This forest area of the sanctuary could be managed and conserved for multi sectional uses like ecotourism, forest ecology, research and education rather than a Bird sanctuary.<#LINE#>Lovejoy T.E. (1980).@The Global 2000 Report to the President.@The Technical Report, Penguin, New York, 2, 327-332.@No$IUCN (1971).@The Ramser Conferences: Final Act of the International Conference on the Conservation of Wetland and Waterfowl, Special Suppl.@IUCN Bull, 2(9), 1-4.@No$Sabu T. (2009).@Wetlands and Biodiversity conservation in Kerala, Paper presented on state level seminar on siginificance of Wetlands in Kerala’s Development.@Kerala state land use Board, Vikas Bhavan, Thiruvananthapuram, 7-13.@No$Gamble J.S. (1935).@Flora of the Presidency of Madras, (Govt: of India Copy right in 1958).@Botanical Survey of India, I to III, 1389.@No$Biswae K. and Calder C. (1954).@Hand Book of Common Aquatic and Marsh Plant of India and Burma.@Manager of Publications, Delhi.@Yes$Subramanyam K. (1962).@Aquatic Angiosperms.@Coucil of Scientific and Industrial Research, India, 190.@Yes$Nayar T.S, Rasiya Beegam A. and Sibi M. (2014).@Flowering Plants of the Western Ghats, India.@Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala.@Yes$Rao Rama (1914).@Flowering Plants of Travancore.@Govt: Press Trivandram, XIV-495.@Yes$Caius J.F. (1986).@The medicinal and poisonous plants of India.@Scientific Publishers Jodhpur, India, 528.@Yes$Kirtikar K.R. and Basu B.D. (1993).@Indian Medicinal Plants.@Periodical Experts Book Agency. New Delhi, I to IV, 279.@No$Srivasthava R.C. (1989).@Drug Plants of Central india.@(An Inventiry), Today and Tomorrow Publishers and Printers. New Delhi, 258.@No$Sivarajan and Balachandran Indira (1994).@Ayurvedic Drugs and Their Plant Sources.@Oxford & IBH Publishing. New Delhi, 570.@Yes$IUCN Red List Plants of India (IUCN 2012).@IUCN Red List of Threatened Species.@Version 2012.2. www.iucnredlist.org. download on 10 January 2013.@No$Vasishta P.C. (1992).@Taxonomy of Angiosperms.@R. Chand & Co. Publishers, New Delhi-2.1-184.@No <#LINE#>Effect of natural and synthetic dyes on human health<#LINE#> Zahra@Naseem,Kalim@Imran,Khalid Saeed@Muhammad,Mumtaz@Zilwa,Amjad@Naima,Nisa@Alim-un,Hina@Sajila,Masood@Shahid,Ahmed@Ijaz,Ashraf@Muhammad <#LINE#>23-29<#LINE#>4.ISCA-IRJBS-2017-081.pdf<#LINE#>Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan and College of Earth and Environmental Sciences, University of the Punjab, Lahore@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan@Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore@Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan@Pakistan Council of Scientific and Industrial Research Laboratories Complex, Ferozepur Road, Lahore, 54600, Pakistan<#LINE#>21/8/2017<#LINE#>6/10/2017<#LINE#>Food dyes are added to enhance the quality perception of the food. Dyes are of two categories natural dyes and artificial dyes. Natural dyes are extracted from the plants or animal source. So they are quite expensive and not available in large quantity. Artificial dyes are petroleum based products and constitutes aromatic rings. Dyes are extensively used in food items like candies, bakery, confectionery, drinks, rice, cooked as well as processed foods. Despite of its extensive use the ill-health situations caused by over-dosed use of dyes must not be underlooked. Multiple disorders are related to the toxicity of the artificial dyes. Dyes are potentially mutagenic either directly or indirectly.<#LINE#>Barrows J.N., Lipman A.L. and Bailey C.J. (2003).@Color additives: FDA’s regulatory process and historical perspectives.@Food Safety Magazine, 1.@Yes$Tripathi M., Khanna S.K. and Das M. (2007).@Surveillance on use of synthetic colours in eatables vis a vis Prevention of Food Adulteration Act of India.@Food Control, 18(3), 211-219.@Yes$Yang B., Gao Y., Liu X., Li Y. and Zhao J. (2009).@Adsorption characteristics of crocin in the extract of gardenia fruits (Gardenia jasminoides Ellis) on macroporous resins.@Journal of food process engineering, 32(1), 35-52.@Yes$Dixit S., Purshottam S.K., Gupta S.K., Khanna S.K. and Das M. (2010).@Usage pattern and exposure assessment of food colours in different age groups of consumers in the State of Uttar Pradesh, India.@Food Additives and Contaminants, 27(2), 181-189.@Yes$Sharma V., McKone H.T. and Markow P.G. (2010).@A global perspective on the history, use, and identification of synthetic food dyes.@Journal of Chemical Education, 88(1), 24-28.@Yes$Zahra N., Alim-un-Nisa I.K., Fatima S., Khan H., Akhlaq F., Butt I.F. and Hina S. (2016).@Identification of Synthetic Food Dyes in Various Candies.@Pak. J. Biochem. Mol. Biol, 49(1), 09-17.@Yes$Abdullah S.U., Badaruddin M., Sayeed S.A., Ali R. and Riaz M.N. (2008).@Binding ability of Allura Red with food proteins and its impact on protein digestibility.@Food chemistry, 110(3), 605-610.@Yes$Chequer F.M.D., de Paula Venâncio V., Bianchi M.D.L.P. and Antunes L.M.G. (2012).@Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells.@Food and Chemical Toxicology, 50(10), 3447-3451.@Yes$Zhao J., Wu T., Wu K., Oikawa K., Hidaka H. and Serpone N. (1998).@Photoassisted degradation of dye pollutants. 3. Degradation of the cationic dye rhodamine B in aqueous anionic surfactant/TiO2 dispersions under visible light irradiation: evidence for the need of substrate adsorption on TiO2 particles.@Environmental science & technology, 32(16), 2394-2400.@Yes$Shakir K., Elkafrawy A.F., Ghoneimy H.F., Beheir S.G.E. and Refaat M. (2010).@Removal of rhodamine B (a basic dye) and thoron (an acidic dye) from dilute aqueous solutions and wastewater simulants by ion flotation.@Water research, 44(5), 1449-1461.@Yes$Suzuki S., Shirao M., Aizawa M., Nakazawa H., Sasa K. and Sasagawa H. (1994).@Determination of synthetic food dyes by capillary electrophoresis.@Journal of Chromatography A., 680(2), 541-547.@Yes$Chanlon S., Joly-Pottuz L., Chatelut M., Vittori O. and Cretier J.L. (2005).@Determination of Carmoisine, Allura red and Ponceau 4R in sweets and soft drinks by Differential Pulse Polarography.@Journal of Food Composition and Analysis, 18(6), 503-515.@Yes$Puttemans M.L., Dryon L. and Massart D.L. (1984).@Extraction of organic acids by ion-pair formation with tri-n-octylamine.@Part V. Simultaneous determination of synthetic dyes, benzoic acid, sorbic acid, and saccharin in soft drinks and lemonade syrups. Journal-Association of Official Analytical Chemists, 67(5), 880-885.@Yes$Purba M.K., Agrawal N. and Shukla S.K. (2015).@Detection of Non-Permitted Food Colors in Edibles.@Journal of Forensic Research, (S4), 1.@Yes$Authority European Food Safety (2015).@Refined exposure assessment for Ponceau 4R (E 124).@EFSA Journal, 13(4).@Yes$Horowitz C.R., Colson K.A., Hebert P.L. and Lancaster K. (2004).@Barriers to buying healthy foods for people with diabetes: evidence of environmental disparities.@American Journal of Public Health, 94(9), 1549-1554.@Yes$Giusti M.M. and Wrolstad R.E. (2003).@Acylated anthocyanins from edible sources and their applications in food systems.@Biochemical Engineering Journal, 14(3), 217-225.@Yes$Obón J.M., Castellar M.R., Alacid M. and Fernández-López J.A. (2009).@Production of a red–purple food colorant from Opuntia stricta fruits by spray drying and its application in food model systems.@Journal of Food Engineering, 90(4), 471-479.@Yes$McCann D., Barrett A., Cooper A., Crumpler D., Dalen L., Grimshaw K., Kitchin E., Lok K., Porteous L., Prince E. and Sonuga-Barke E. (2007).@Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: a randomised, double-blinded, placebo-controlled trial.@The lancet, 370(9598), 1560-1567.@Yes$Mapari S.A., Nielsen K.F., Larsen T.O., Frisvad J.C., Meyer A.S. and Thrane U. (2005).@Exploring fungal biodiversity for the production of water-soluble pigments as potential natural food colorants.@Current Opinion in Biotechnology, 16(2), 231-238.@Yes$Watanabe T. and Terabe S. (2000).@Analysis of natural food pigments by capillary electrophoresis.@Journal of Chromatography A, 880(1), 311-322.@Yes$Nagai S. (1959).@Induction of the respiration-deficient mutation in yeast by various synthetic dyes.@Science, 130(3383), 1188-1189.@Yes$Saleem N. and Umar Z.N. (2013).@Survey on the use of synthetic Food Colors in Food Samples procured from different educational institutes of Karachi city.@Journal of tropical life science, 3(1), 1-7.@Yes$Brown M.A. and De Vito S.C. (1993).@Predicting azo dye toxicity.@Critical reviews in environmental science and technology, 23(3), 249-324.@Yes$Alim N., Zahra N. and Akhlaq F. (2015).@Detection of Sudan dyes in different spices.@Pakistan Journal of Food Sciences, 25(3), 144-149.@Yes$Combes R.D. and Haveland-Smith R.B. (1982).@A review of the genotoxicity of food, drug and cosmetic colours and other azo, triphenylmethane and xanthene dyes.@Mutation Research/Reviews in genetic toxicology, 98(2), 101-243.@Yes$Brown J.P., Roehm G.W. and Brown R.J. (1978).@Mutagenicity testing of certified food colors and related azo, xanthene and triphenylmethane dyes with the Salmonella/microsome system.@Mutation Research/ Fundamental and Molecular Mechanisms of Mutagenesis, 56(3), 249-271.@Yes$Kanarek R.B. (2011).@Artificial food dyes and attention deficit hyperactivity disorder.@Nutrition reviews, 69(7), 385-391.@Yes$Millichap J.G. and Yee M.M. (2012).@The diet factor in attention-deficit/hyperactivity disorder.@Pediatrics, 129(2), 330-337.@Yes$Bateman B., Warner J.O., Hutchinson E., Dean T., Rowlandson P., Gant C., Grundy J., Fitzgerald C. and Stevenson J. (2004).@The effects of a double blind, placebo controlled, artificial food colourings and benzoate preservative challenge on hyperactivity in a general population sample of preschool children.@Archives of Disease in Childhood, 89(6), 506-511.@Yes$Stevens L.J., Kuczek T., Burgess J.R., Hurt E. and Arnold L.E. (2011).@Dietary sensitivities and ADHD symptoms: thirty-five years of research.@Clinical pediatrics, 50(4), 279-293.@Yes$Ashfaq N. and Masud T. (2002).@Surveillance on artifical colours in different ready to eat foods.@Pakistan J Nutr, 5, 223-225.@Yes$Siva R. (2007).@Status of natural dyes and dye-yielding plants in India.@Current Science, 92(7), 916-925.@Yes