@Research Paper <#LINE#>The effects of Pulmonary administration of Fe2O3 Nanoparticles on the Lung Tissue in Wistar Rat<#LINE#>Momayez@Monireh,Mohammad@Fazilati<#LINE#>1-5<#LINE#>1.ISCA-IRJBS-2013-202.pdf<#LINE#>Department of Biology, Payam nor university of Isfahan, Isfahan, IRAN <#LINE#>6/8/2013<#LINE#>20/2/2014<#LINE#> Iron oxide nanoparticles are magnetic nanoparticles have widespread application in MRI and heat therapy of cancer as contrast elements. They also used effectively to drug and gene delivery because of effective penetrating to the cells and tissues. However, these features cause Fe nanoparticles have toxic effects that not studied yet completely. In this study, effects of iron oxidenano particles on lung tissue in adult male wistar rats were studied. We used pulmonary inhalation method for nanoparticle administration and used ether as a helper. Our results showed administrated nanoparticles penetrated to the circulation rapidly and created serious inflammation in lung tissues. This study used two different nanoparticle doses (20 and 40 mg/kg) and two different exposing number (7 and 14 times).Histological studies show ednano particle treatment of rats cause pulmonary emphysema, interstitial hyperemia and inflammation in lung. By increasing the administrated dose lung tissue showed all of mentioned symptoms by increased intensity. Nanoparticle exposing causes presence of neutrophils, lymphocytesandeosinophils in the lung tissue that confirmed there is a serious pathologic condition. <#LINE#> @ @ Jani P., Halberd G. W., Langridge J., Florence A. T., Nanoparticle uptake by the gastrointestinal mucosa: quantitation and particle dependency, J. Pharm. Pharmacol.,42, 821-826 (1990) @No $ @ @ Indira T. K., Lakshmi P. K., Magnetic Nanoparticles–A Review, Int J Pharm,, 1035-1042 (2010) @No $ @ @ Weinstein J. S., Varallyay C. G., Dosa E., Gahramanov S., Hamilton B., Rooney W. D., Muldoon L. L., Neuwelt E. A., Superparamagnetic iron oxide nanoparticles: diagnostic magnetic resonance imaging and potential therapeutic applications in neurooncology and central nervous system inflammatory pathologies, a review, J. Cereb. Blood Flow Metab.,30, 15-35 (2010) @No $ @ @ McAuliffe M. E., Perry M. G., Are nanoparticles potential male reproductive toxicant? A literature review, Nanotoxicol., , 204-210 (2007) @No $ @ @ YousefiBabadi V., Najafi L., Najafi A., Gholami H., BeigiZarji M. E., Golzadeh J., Amraie E., Shirband A., Evaluation of iron oxide nanoparticles effects on tissue and enzymes of liver in rats, J. Pharma. Biomed. Sci.,23, 1-5 (2012) @No $ @ @ Gupta A. k., Gupta M., Synthesis and Surfaceengineeringof iron oxide nanoparticles and their biomedical applications, Biomater, 26, 3995-4021 (2005) @No $ @ @ Liang X. J., Chen C., Zhao Y., Jia L., Liang X. J., Chen C., Zhao Y., Jia L., Wang P. C, Biopharmaceutics and Therapeutic Potential of Engineered Nanomaterials, Curr Drug Metab,, 1-13 (2008) @No $ @ @ Espanani H. R, Fazilati M., Sadeghi L., YousefiBabadi V.,Bakhshiani S. Amraie E., Investigation the Zinc Oxide Nanoparticle’s Effect on Sex Hormones and Cholesterol in Rat, Int. Res. J. Biological Sci,, 1-6 (2013) @No $ @ @ Zhu M. T., Feng W.Y., Wang Y., Wang B., Wang M., Ouyang H., Zhao Y. L. Chai Z. H., Particokinetics and Extrapulmonary Translocation of IntratracheallyInstilled Ferric Oxide Nanoparticles in Rats and the Potential HealthRisk Assessment, ToxicoloSci,107, 342–351 (2009) @No $ @ @ Buzea C., Ivan I., Pacheco Blandino I. I., Robbie K., Nanomaterials and nanoparticles:Sources and toxicity, Biointerphases, , 17-172 (2007) @No $ @ @ Yah C. S., Simate G. S., Iyuke S. E., Nanoparticles toxicity and their routes of exposures, Pak J Pharm Sci.,25, 477-91 (2012) @No $ @ @ Driscoll K., Costa D., Hatch G., Henderson R., Intracheal Instillation as an Exposure Technique for Respiratory Tract Toxicity: Uses and Limitation, ToxicoloSci, 55, 24-35 (2000) @No $ @ @ Borm P. J., Kreyline W., Toxicological hazards of inhaled nanoparticles potential implications durg delivery, J NanosciNanotechnol.,, 521-531 (2004) @No $ @ @ Roursgaard M., Inflammatory effects of nanoparticles in lungs of mice, PhD thesis, faculty of health sciences, university of Copenhagen (2009) @No $ @ @ Dhandapani S., Subramanian V. R., Rajagopal S., Namasivayam N., Hypolipidemic Effects of CuminumCyminuml on Alloxan-induced diabetic rats, Pharmacol Res., 46, 251-255 (2002) @No $ @ @ Berry c. c., Charles S., Wells S., Dalby M. J., Curtis A.S., The influnence of transferrin stabilized magnetic nanoparticles on human dermal fibroblasts in culture, Int. J. Pharm.,269, 211-225 (2004) @No $ @ @ YousefiBabadi V., Amraeai E., Salehh H., Sadeghi L., Najafi L., Fazilati M., Evaluation of Iron Oxide nanoparticles effects on tissue and Enzymes ofThyroid in Rats, Int. Res. J. Biological Sci,, 67-69 (2013) @No $ @ @ Mital, G. S., Manoj, T. A., Review of Tio2 nanoparticles, Chinese Science Bulletin, 56, 1639-1657 (2011) @No $ @ @ Rezaei-Zarchi S., Rad S., Soezi M., Negahdary M., Amrollahi F., Mazaheri G.R., Borna H., Investigation of The effect of Fe2O3 nanoparticles on the blood cells and liver enzymes inmale rat, Adv Environ Biol, , 334-342 (2012) @No $ @ @ Park E. J., Cho W. S., Jeong j., Yi J. H., Choi k., kim Y., Park K., Induction ofinflammatory responses in mice treated with Crium oxide Nanoparticles by intratracheal instillation, J Health Sci, 56, 387-396 (2010) @No $ @ @ Szalasy B., Ogden J. A., Strawbridge R. R., ZE Pierce R., Hoopes P. J., Iron 0xide nanoparticles and their toxicological effects: in vivo and invitro studies. , 7181-7181 (2009) @No $ @ @ YousefiBabadi V., Amraeai E., Salehh H., Sadeghi L., Najafi L., Fazilati M., Evaluation of Iron Oxide nanoparticles effects on tissue and Enzymes of Thyroid in Rats, Int. Res. J. Biological Sci, , 67-69 (2013) @No $ @ @ Dodd N., Jha A. N.,Titanium dioxide induced cell damage:A Proposed of the carboxyl radical, Mutat Res, 660, 79-82 (2008) @No $ @ @ Wang L., Ding W., Zhang, F., Acute Toxicity of Ferric Oxide and Zinc OxideNanoparticles in Rats, J NanosciNanotechnol,10, 8617–8624 (2010) @No <#LINE#>Phytoremediation Efficiency of Sunflower Crop with Textile and Dye Industry Liquid and Solid waste and Amendments on Phosphorus Status of Polluted Soil Habitat<#LINE#>Parameswari@M.<#LINE#>6-9<#LINE#>2.ISCA-IRJBS-2013-231.pdf<#LINE#>Department of Environmental Sciences, Tamilnadu Agriculture University, Coimbatore, Tamilnadu, INDIA<#LINE#>6/8/2013<#LINE#>20/2/2014<#LINE#>Iron oxide nanoparticles are magnetic nanoparticles have widespread application in MRI and heat therapy of cancer as contrast elements. They also used effectively to drug and gene delivery because of effective penetrating to the cells and tissues. However, these features cause Fe nanoparticles have toxic effects that not studied yet completely. In this study, effects of iron oxidenano particles on lung tissue in adult male wistar rats were studied. We used pulmonary inhalation method for nanoparticle administration and used ether as a helper. Our results showed administrated nanoparticles penetrated to the circulation rapidly and created serious inflammation in lung tissues. This study used two different nanoparticle doses (20 and 40 mg/kg) and two different exposing number (7 and 14 times).Histological studies show ednano particle treatment of rats cause pulmonary emphysema, interstitial hyperemia and inflammation in lung. By increasing the administrated dose lung tissue showed all of mentioned symptoms by increased intensity. Nanoparticle exposing causes presence of neutrophils, lymphocytesandeosinophils in the lung tissue that confirmed there is a serious pathologic condition. <#LINE#> @ @ Jani P., Halberd G. W., Langridge J., Florence A. T., Nanoparticle uptake by the gastrointestinal mucosa: quantitation and particle dependency, J. Pharm. Pharmacol.,42, 821-826 (1990) @No $ @ @ Indira T. K., Lakshmi P. K., Magnetic Nanoparticles–A Review, Int J Pharm,, 1035-1042 (2010) @No $ @ @ Weinstein J. S., Varallyay C. G., Dosa E., Gahramanov S., Hamilton B., Rooney W. D., Muldoon L. L., Neuwelt E. A., Superparamagnetic iron oxide nanoparticles: diagnostic magnetic resonance imaging and potential therapeutic applications in neurooncology and central nervous system inflammatory pathologies, a review, J. Cereb. Blood Flow Metab.,30, 15-35 (2010) @No $ @ @ McAuliffe M. E., Perry M. G., Are nanoparticles potential male reproductive toxicant? A literature review, Nanotoxicol., , 204-210 (2007) @No $ @ @ YousefiBabadi V., Najafi L., Najafi A., Gholami H., BeigiZarji M. E., Golzadeh J., Amraie E., Shirband A., Evaluation of iron oxide nanoparticles effects on tissue and enzymes of liver in rats, J. Pharma. Biomed. Sci.,23, 1-5 (2012) @No $ @ @ Gupta A. k., Gupta M., Synthesis and Surfaceengineeringof iron oxide nanoparticles and their biomedical applications, Biomater, 26, 3995-4021 (2005) @No $ @ @ Liang X. J., Chen C., Zhao Y., Jia L., Liang X. J., Chen C., Zhao Y., Jia L., Wang P. C, Biopharmaceutics and Therapeutic Potential of Engineered Nanomaterials, Curr Drug Metab,, 1-13 (2008) @No $ @ @ 5Nanoparticle’s Effect on Sex Hormones and Cholesterol in Rat, Int. Res. J. Biological Sci,, 1-6 (2013) @No $ @ @ Zhu M. T., Feng W.Y., Wang Y., Wang B., Wang M., Ouyang H., Zhao Y. L. Chai Z. H., Particokinetics and Extrapulmonary Translocation of IntratracheallyInstilled Ferric Oxide Nanoparticles in Rats and the Potential HealthRisk Assessment, ToxicoloSci,107, 342–351 (2009) @No $ @ @ Buzea C., Ivan I., Pacheco Blandino I. I., Robbie K., Nanomaterials and nanoparticles:Sources and toxicity, Biointerphases, , 17-172 (2007) @No $ @ @ Yah C. S., Simate G. S., Iyuke S. E., Nanoparticles toxicity and their routes of exposures, Pak J Pharm Sci.,25, 477-91 (2012) @No $ @ @ Driscoll K., Costa D., Hatch G., Henderson R., Intracheal Instillation as an Exposure Technique for Respiratory Tract Toxicity: Uses and Limitation, ToxicoloSci, 55, 24-35 (2000) @No $ @ @ Borm P. J., Kreyline W., Toxicological hazards of inhaled nanoparticles potential implications durg delivery, J NanosciNanotechnol.,, 521-531 (2004) @No $ @ @ Roursgaard M., Inflammatory effects of nanoparticles in lungs of mice, PhD thesis, faculty of health sciences, university of Copenhagen (2009) @No $ @ @ Dhandapani S., Subramanian V. R., Rajagopal S., Namasivayam N., Hypolipidemic Effects of CuminumCyminuml on Alloxan-induced diabetic rats, Pharmacol Res., 46, 251-255 (2002) @No $ @ @ Berry c. c., Charles S., Wells S., Dalby M. J., Curtis A.S., The influnence of transferrin stabilized magnetic nanoparticles on human dermal fibroblasts in culture, Int. J. Pharm.,269, 211-225 (2004) @No $ @ @ YousefiBabadi V., Amraeai E., Salehh H., Sadeghi L., Najafi L., Fazilati M., Evaluation of Iron Oxide nanoparticles effects on tissue and Enzymes ofThyroid in Rats, Int. Res. J. Biological Sci,, 67-69 (2013) @No $ @ @ Mital, G. S., Manoj, T. A., Review of Tio2 nanoparticles, Chinese Science Bulletin, 56, 1639-1657 (2011) @No $ @ @ Rezaei-Zarchi S., Rad S., Soezi M., Negahdary M., Amrollahi F., Mazaheri G.R., Borna H., Investigation of The effect of Fe2O3 nanoparticles on the blood cells and liver enzymes inmale rat, Adv Environ Biol, , 334-342 (2012) @No $ @ @ Park E. J., Cho W. S., Jeong j., Yi J. H., Choi k., kim Y., Park K., Induction ofinflammatory responses in mice treated with Crium oxide Nanoparticles by intratracheal instillation, J Health Sci, 56, 387-396 (2010) @No $ @ @ Szalasy B., Ogden J. A., Strawbridge R. R., ZE Pierce R., Hoopes P. J., Iron 0xide nanoparticles and their toxicological effects: in vivo and invitro studies. , 7181-7181 (2009) @No $ @ @ YousefiBabadi V., Amraeai E., Salehh H., Sadeghi L., Najafi L., Fazilati M., Evaluation of Iron Oxide nanoparticles effects on tissue and Enzymes of Thyroid in Rats, Int. Res. J. Biological Sci, , 67-69 (2013) @No $ @ @ Dodd N., Jha A. N.,Titanium dioxide induced cell damage:A Proposed of the carboxyl radical, Mutat Res, 660, 79-82 (2008) @No $ @ @ Wang L., Ding W., Zhang, F., Acute Toxicity of Ferric Oxide and Zinc OxideNanoparticles in Rats, J NanosciNanotechnol,10, 8617–8624 (2010) @No <#LINE#>An Investigation on Behavioral Pattern of Homicide in People with Psychotic Disorders, People with Personality Disorders and People without any Mental Disorders<#LINE#>Mehdi@SaberiSeyed,Jaber@Gharehdaghi,Armindokht@Ahmadi,Masafi@Saideh,Omid@Rezaei<#LINE#>10-14<#LINE#>3.ISCA-IRJBS-2013-295.pdf<#LINE#>Legal medicine research center, Legal medicine organization, Tehran, Iran Department of psychology, Kish International Branch, Islamic Azad University, Kish, IRAN Department of Psychiatry, University of Social Welfare & Rehabilitation Sciences, Tehran, IRAN <#LINE#>26/11/2013<#LINE#>9/1/2014<#LINE#>Homicide is the act of a human killing another human. It is not always a punishable act under criminal law, and is different to murder from a formal legal point of view. The mental state of the perpetrator of these crimes differs from that of one who commits murder. The purpose of this study is to reach a criminal behavior pattern in three dimensions (i.e. motivation, criminal act, and behavior after the crime) which can solve some problems in process of judicial judgments and precise decision making. This research is a cross-sectional survey on a statistical society of all the people who have committed human killing since 2001 through 2009 and have been referred to Tehran Legal Medicine center to be observed by psychiatrists. Sampling method is targeted in which 230 people were selected randomly. Required information was collected through related documents archive. Statistical tests of x, data was analyzed and in terms of previous motivations, no significant difference was seen (p0.05, x=1.75). In terms of lethal weapons, a significant difference was seen (p0.05, =6.156). Also, in terms of the behavior after crime, some significant differences were seen in the groups (p0.05, =21.775). Every 3 groups of people with psychotic disorders, persons with personality disorders, and healthy ones have committed homicide with previous motivation. In terms of lethal tools, health ones often use lethal weapons to do homicide; and in terms of the behavior after crime, the psychotics mostly have unusual behaviors and do not leave the crime location. <#LINE#> @ @ Cosmides L. and Tooby J., Are humans good intuitive statisticians after all? Rethinking some conclusions from the literature on judgment under uncertainty, Cognition,58, 1-73 (1995) @No $ @ @ Dawson J.M. and Langan P.A., Murder in families, Bureau of Justice Statistics special report,Washington, DC: U.S. Department of Justice; (1994) @No $ @ @ Personality disorders, Wikipedia, free encyclopedia website, http://en.wikipedia.org/wiki/Personality_disorders, Accessed September 16 ( 2012) @No $ @ @ Davis J.A., The use of behavioral scientists in low enforcement, The Low Enforcement Quarterly; January, 20-28 (1993) @No $ @ @ Turvey B.E., Criminal Profiling: An Introduction to Behavioral Evidence Analysis,San Diego: Academic Press; (1999) @No $ @ @ Homicide Wikipedia, free encyclopedia website, http://en.wikipedia.org/wiki/Homicide, Accessed September 6, (2012) @No $ @ @ Ganji H., General Psychology Principles, Tehran: Ravan; (1998) @No $ @ @ Crime, Wikipedia, free encyclopedia website. http://en.wikipedia.org/wiki/Criminal_act. Accessed September 14 (2012) @No $ @ @ Santtila P., Häkkänen H. and Canter D., et al. Classifying Homicide Offenders and Predicting their Characteristics from Crime Scene Behavior, Scand J Psychol., 44(2), 107-118 (2003) @No $ @ @ Robert D., Keppel R.D. and Walter R., Profiling Killers: A Revised Classification Model for Understanding Sexual Murder, Int J Offender Ther Comp Criminol,43(4), 417-437 (1999) @No $ @ @ Richard-Devantoy S., Chocard A.S. and Bourdel M.C. et al., [Homicide and major mental disorder: what are the social, clinical, and forensic differences between murderers with a major mental disorder and murderers without any mental disorder?], Encephale, 35(4), 304-314 (2009) @No $ @ @ Disease, Wikipedia, free encyclopedia website. http://en.wikipedia.org/wiki/Disease, Accessed September 17 (2012) @No <#LINE#>Gene Expression Pattern of Insulin-Like Growth Factor-I Receptor on Mesenchymal Stem Cells Induced by Tumor Necrosis Factor-a<#LINE#>Zeinab@Sahraian,Maryam@Ayatollahi,Ramin@Yaghobi,Mehrdad@Shariati<#LINE#>15-20<#LINE#>4.ISCA-IRJBS-2013-299.pdf<#LINE#>Department of biology, Science and Research Branch, Islamic Azad University, Fars, IRAN @ Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IRAN @ Department of biology, Islamic Azad University, Kazerun, IRAN<#LINE#>1/12/2013<#LINE#>20/2/2014<#LINE#> Cell-based therapy has been implicated in treatment in a wide range of diseases. Mesenchymal stem cells from various sources such as bone marrow are available. These cells are one of the major candidates in cell therapy. The production of insulin-like growth factor-I increases in the regenerating organ. The insulin-like growth factor-I in liver regeneration is effective after binding to insulin-like growth factor-I receptor. We hypothesized that tumor necrosis factor- stimulate mesenchymal stem cells to cause insulin-like growth factor-I receptor expression. Bone marrow was aspirated from human normal donor after informing consent. Cells were isolated and cultured. Identification of cells with flow cytometric analysis and functional tests were performed. Fourth passage cells were treated with tumor necrosis factor- at different doses (1 ng/mL and 10 ng/mL) and incubated at different times (2, 10, 24 and 48 hours). Insulin-like growth factor–I receptor gene expression was investigated using real time-polymerase chain reaction technique. Flow cytometric analysis showed that the human bone marrow mesenchymal stem cells were positive for CD90 and negative for CD45 and CD80. The insulin-like growth factor-I receptor gene expression was increased in tumor necrosis factor- treated in comparison with untreated cells. Increase gene expression pattern of insulin-like growth factor-I receptor in human bone marrow derived mesenchymal stem cells may be used for clinical stem cell therapy in acute liver failure. <#LINE#> @ @ Almeida G, Zanjani E, and. Porada Ch , Bone marrow stem cells and liver regeneration, Experimental Hematology, 38,574-580 (2010) @No $ @ @ Minguell J. and Erices A. Mesenchymal stem cells and the treatment of cardiac disease, Exp Biol Med., 231,39-49 (2006) @No $ @ @ Mctaggart S, Atkinson K. Mesenchymal stem cells: immunobiology and therapeutic potential in kidney disease, Nephrology, 12,44-52(2007) @No $ @ @ Friedenstein A, Piatetzky S, Petrakova K. Osteogenesis in transplants of bone marrow cells, J Embryol Exp Morphol.,16,38 1-390 (1966) @No $ @ @ Sato Y, et al. Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood., , 756-63 (2005) @No $ @ @ Leroith D. Insulin-like growth factors, N Engl J Med., 336, 633-640 (1997) @No $ @ @ Daughaday W, Rotwein P. Insulin-like growth factors I and II: Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations, Endocr Rev., 10, 68-91 (1989) @No $ @ @ Valentinis B, Baserga R. IGF-I receptor signaling in transformation and differentiation, Mol Pathology.,54,133-137(2001) @No $ @ @ Fausto N, Laird A, Webber E .1.Liver regeneration. 2. Role of growth factors and cytokines in hepatic regeneration, FASEB J., ,1527-1536 (1995) @No $ @ @ Cressman D, et al. Liver failure and defective hepatocyte regeneration in interleukin-6-deficient mice, Science., 274, 1379-1383 (1996) @No $ @ @ Tracy K. Tumor necrosis factor. In: Cytokine in health and disease, New York: Marcel Dekker., 223-240 (1997) @No $ @ @ 20mesenchymal stem cells based on the rat samples, World J Stem Cells., ,1-8 (2012) @No $ @ @ 4.Davatchi F., et al. Mesenchymal stem cell therapy for knee osteoarthritis, Int J Rheum Dis.,14, 211-215 (2011) @No $ @ @ Volarevic V, Arsenijevic N, Lukic M. Mesenchymal stem cell treatment of the complications of diabetes mellitus, Stem Cells., 29, 5-10 (2011) @No $ @ @ Bao X, et al. Transplantation of human bone marrow-derived mesenchymal stem cells promotes behavioral recovery and endogenous neurogenesis after cerebral ischemia in rats, Brain Res., 1367, 103-13 (2011) @No $ @ @ Jones E. and Gonagle D., Human bone marrow mesenchymal stem cells in vivo. Rheumatology., 11,1-6 (2007) @No $ @ @ Lundberg J., et al., Endovascular transplantation of stem cells to the injured rat CNS, Neuroradiology., 51, 661-7 (2009) @No $ @ @ Cheng H., et al. Osteogenic activity of the fourteen types of human bone morphogenic proteins (BMPs), J Bone Surg Am., 85, 1544-1552 (2003) @No $ @ @ Hwang J., et al., Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood and bone marrow, J Korean Med Sci., 24, 547-54 (2009) @No $ @ @ Look D., et al., Selective induction of intercellular adhesion molecule-1 by interferon gamma in human airway epithelial cells, Am. J. Physiol., 263, 79-87 (1992) @No $ @ @ Lieke C, et al. Mesenchymal stem cells respond to TNF but do not produce TNF, Journal of Leukocyte Biology., 283-289 (2010) @No $ @ @ Bonizzi G. and Karin M., The two NF-kappaB activation pathways and their role in innate and adaptive immunity, Trends Immunol ., 25, 280-288 (2004) @No $ @ @ Henness S, et al. IL-17A acts via p38 MAPK to increase stability of TNF--induced IL-8 mRNA in human ASM, Am J Physiol Lung Cell Mol Physiol., 290, 1283-1290 (2006) @No $ @ @ Jones J. and Clemmons D. Insulin-like growth factors and their binding proteins, Biological Actions. Endocr Rev., , 3-34 (1995) @No $ @ @ Soos M, et al. Hybrid insulin/insulin-like growth factor-I receptors bind insulin-like growth factor-I but not insulin with high affinity, Biochem J., 290, 419-426 (1993) @No $ @ @ Treadway J., Frattali A. and Pessin J., Intramolecular subunit interactions between insulin and insulin-like growth factor-I ab half receptors induced by ligand and Mn/Mg ATP binding, Biochemistry., 31, 11801-11805 (1992) @No <#LINE#>Management of Diabetes Mellitus among Elderly Men and Women – Impact of Intervention Programme<#LINE#>K.S.@Roopa,G.@RamaDevi<#LINE#>21-25<#LINE#>5.ISCA-IRJBS-2013-317.pdf<#LINE#> Dept. of Human Development and Research Centre, Smt. V.H.D. Central Institute of Home Science, Bangalore University, Bangalore – 560 001, Karnataka, INDIA <#LINE#>20/12/2013<#LINE#>20/2/2014<#LINE#>The present research aimed at studying the effect of an educational module as an intervention programme in the management of Diabetes Mellitus among the elderly with regard to the improvement in their knowledge, attitude and practices. The sample constituted 80 elderly of whom 40 were men and 40 were women in the age group of 65 to 76 years. Structured Interview Schedule (SIS) developed by the investigators on knowledge, attitude and practices (KAP) with regard to diabetes was used for assessment. The module of educational programme was developed by the investigators for the benefit of diabetic people covering different aspects in the management of the disease. A repeated measure design was followed; accordingly, the sample were administered SIS to assess their initial KAP and they were provided intervention programme for a period of two months. Later, two months after the intervention, post assessment was conducted administering the same SIS. A comparison of pre and post test scores on KAP regarding diabetes was made. The statistical significance of mean difference in the scores was carried out using ‘t’ ratios. The findings revealed that both men and women respondents and the two age groups showed higher scores after intervention, on KAP regarding diabetes, than the pre intervention scores and individual differences in the score was also reduced considerably during post test. <#LINE#> @ @ American Geriatrics Society. Beers Criteria Update Expert Panel. American Geriatrics Society updated Beers criteria for potentially inappropriate medication use in older adults, Journal of American Geriatric Society, 60, 616–631 (2012) @No $ @ @ Ramachandran A, Snehalatha C, Kapur A, Vijay V, Mohan V,Das AK, Rao PV, Yajnik CS, Prasanna Kumar KM, Nair JD. Diabetes Epidemiology Study Group in India (DESI) : High prevalence of diabetes and impaired glucose tolerance in India: National Urban Diabetes Survey, Diabetologia, 44, 1094-1101 (2001) @No $ @ @ Araki E. and Miyazaki J.I., Metabolic disorder in diabetes mellitus : impact of mitochondrial function and oxidative stress on diabetes and its complications, Antioxidants and Redox Signalling, 9(3), 289-291 (2007) @No $ @ @ Behera B., Yadav D. and Sharma M.C., Effect of an Herbal Fromulation (Indrayanadi Yog) on Blood Glucose level., Int. Res. J. Biological Sci., 2(4), 67-71(2013) @No $ @ @ Azizi F., Guoya M.M., Vazirian P., Dolatshati P., Habbiban S., Screening for type 2 diabetes in the Iranian national programme: a preliminary report, East Mediterr Health J., 9, 1122-7 (2003) @No $ @ @ Hussain A., Vaaler S., Sayeed M.A., Mahtab H., Ali S.M., Khan A.K., Type 2 diabetes and impaired fasting blood glucose in rural Bangladesh: a population-based study, Eur J Public Health, 17, 291-6 (2007) @No $ @ @ Mohan V., Mathur P., Deepa R., Deepa M., Shukla D.K., Menon G.R., Urban rural differences in prevalence of self reported diabetes in India – the WHO-ICMR Indian NCD risk factor surveillance, Diabetes Res ClinPract, 80(1), 159-68 (2008) @No $ @ @ Richard S., Jonathan S., Paul Zimmet B., IDF Diabetes Atlas fourth edition, Diabetes and IGT 1-105 (2010) @No $ @ @ Narayan, K.M., Boyle, J.P., and Thompson, J., Lifetime risk for diabetes mellitus in the United States, The Journal of American Medical Association, 290, 1884–1890 (2003) @No $ @ @ Michell Gulabani., Mary John., and Rajesh Isaac Knowledge of diabetes, its treatment and complications amongst diabetic patients in a tertiary care hospital, Indian Journal of Community Medicine, 33(3), 204–206 (2008) @No <#LINE#>Genetic Analysis of Y-chromosomal STRs in Khandayat Population of Odisha, INDIA<#LINE#>B.P.@Nayak,H.@Khajuria,S.@Gupta<#LINE#>26-28<#LINE#>6.ISCA-IRJBS-2014-002.pdf<#LINE#>Amity Institute of Forensic Sciences (AIFS), Amity University, Noida, U.P., INDIA @ Shriram Institute for Industrial Research, New Delhi, INDIA<#LINE#>1/1/2014<#LINE#>19/2/2014<#LINE#>This study was conducted to analyze haplotype diversity Khandayat Community of Odisha, INDIA using Y-chromosome specific short tandem repeat (Y-STR) loci. We collected blood samples from 106 unrelated healthy men of Khandayat Community and isolation of DNA was carried out by standard phenol-chloroform extraction method. Genomic DNA samples were amplified by multiplex-PCR using AmpFl STR Yfiler PCR Amplification Kit for 12 Y-STR loci followed by genotyping. A total of 97 haplotypes were identified among 106 individuals, out of which 93 were unique. Allelic frequency and gene diversity (GD) were calculated for each Y-STR locus. The resulting haplotype diversity (HD) was found to be 0.9798. Discrimination capacity (DC) was observed to be 0.9135. Haplotypes of Khandayat population of Odisha were compared with that of other Indian populations using AMOVA (Analysis of molecular variance) tool in order to measure the genetic relatedness between different populations. <#LINE#> @ @ de Knijff P., Messages through bottlenecks: On the combined use of slow and fast evolving polymorphic markers on the human Y chromosome, Am Jour of Human Genet,67, 1055-1061 (2000) @No $ @ @ Y., Hurles M.E. and Jobling M.A., Haploid chromosomes in molecular ecology: lessons from the human, Mol Ecol, 10, 1599-613 (2001) @No $ @ @ Jobling M.A. and Tyler-Smith C., The human Y chromosome: an evolutionary marker comes of age, Nat Rev Genet,, 598-612 (2003) @No $ @ @ Jobling M.A., Pandya A. and Tyler-Smith C., The Y chromosome in forensic analysis and paternity testing, Int Jour of Legal Med,110, 118-124 (1997) @No $ @ @ Corach D., Figueira R.L., Marino M., Penacino G. and Sala A., Routine Y-STR typing in forensic case-work, Forensic Sci Int,118, 131-135 (2001) @No $ @ @ Gill P., Brenner C., Brinkmann B., Budowle B., Carracedo A., Jobling M.A., de Knijff P., Kayser M., Krawczak M., Mayr W.R., Morling N., Olaisen B., Pascali V., Prinz M., Roewer L., Schneider P.M., Sajantila A. and Tyler-Smith, C. DNA Commission of the International Society of Forensic Genetics: Recommendations on forensic analysis using Y-chromosome STRs, Forensic Sci Int,124, 5-10 (2001) @No $ @ @ de Knijff P., Kayser M., Caglia A., Corach D., Fretwell N., Gehrig C., Graziosi G., Heidorn F., Herrmann S., Herzog B., Hidding M., Honda K., Jobling M., Krawczak M., Leim K., Meuser S., Meyer E., Oesterreich W., Pandya A., Parson W., Penacino G., Perez-Lezaun A., Piccinini A., Prinz M., Schmitt C., Schneider P.M., Szibor R., Teifel-Greding J., Weichhold G.M. and Roewer L., Chromosome Y microsatellites: population genetic and evolutionary aspects, Int J Legal Med, 110(3), 134-149 (1997) @No $ @ @ Bhasin M.K. and Walter H., Genetics of Castes and Tribes of India, Kamla-Raj Enterprises, Delhi (2001) @No $ @ @ Indian Genome Variation Consortium, Genetic landscape of the people of India: a canvas for disease gene exploration, J. 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Ltd, New Delhi (1991) @No $ @ @ Cordaux R., Aunger R., Bentley G., Nasidze I., Sirajuddin S.M. and Stoneking M., Independent origins of Indian caste and tribal paternal lineages, Curr Biol, 14, 231-235 (2004) @No $ @ @ Singh K.S., People of India: An Introduction, Anthropological Survey of India, Calcutta (1992) @No $ @ @ Reich D., Thangaraj K., Patterson N., Price A.L. and Singh L., Reconstructing Indian population history, Nature, 461, 489–495 (2009) @No $ @ @ Bamshad M., Kivisild T., Scott Watkins W., Dixon M.E., Ricker C.E., Rao B.B., Mastan Naidu J., Ravi Prasad B.V., Govinda Reddy P., Rasanayagam A., Papiha S.S., Villems R., Redd, A J., Hammer M.F., Nguyen S.V., Carroll M. L., Batzer M.A. and Jorde L.B, Genetic evidence on the origins of Indian caste populations, Genome Res, 11, 994-1004 (2001) @No $ @ @ Nayak B.P. Khajuria H. and Gupta S., Y-STR Polymorphism among Khandayat Community of Odisha, India, Res. J. Forensic Sci., 1(3), 5-6 (2013) @No $ @ @ Sambrook J., Maccallum P., Russel D., Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Springs Harbour Press, NY (2001) @No $ @ @ Redd A.J., Clifford S.L. and Stoneking M., Multiplex DNA typing of short-tandem-repeat loci on the Y chromosome, Biol Chem, 378(8), 923-7 (1997) @No $ @ @ Nei, M., Molecular Evolutionary Genetics. Columbia University Press, New York (1987) @No $ @ @ Roewer L., Kayser M., Dieltjes P., Nagy M., Bakker E., Krawczak M. and De Knijff P., Analysis of molecular variance (AMOVA) of Y-chromosome-specific microsatellites in two closely related human populations, Hum Mol Genet, 5, 1029-33 (1996) @No $ @ @ Willuweit S., Roewer L., Y chromosome haplotype reference database (YHRD): update, Forensic Sci Int Genet, 1, 83-87 (2007) @No <#LINE#>Isolation and Characterization of Keratinolytic Bacteria from Poultry farm soils<#LINE#>S.A.@Kulkarni,A.R.@Jadhav<#LINE#>29-33<#LINE#>7.ISCA-IRJBS-2014-003.pdf<#LINE#>Department of Microbiology, K.R.P. Kanya Mahavidyalaya, Islampur, Dist- Sangli Maharashtra, INDIA Shri. Jagdishprasad Jhabarmal Tibrewala University, Zhunjhunu, Rajasthan, INDA<#LINE#>9/1/2014<#LINE#>28/2/2014<#LINE#> Keratinolytic microorganisms have a great importance in poultry waste degradation and its bioconversion to compost or animal feed. The present study aimed at selection of keratin degrading bacteria from poultry soil. The poultry farm soil samples were added in basal medium with feathers as a source of carbon and nitrogen. The enriched samples were streaked on nutrient agar and hichromebacillus agar for isolation of keratinolytic bacteria.15 isolates were obtained. All the isolates were screened for their keratinolytic potential on milk agar. Out of fifteen isolates five were keratinolytic.Five isolates were further studied for feather degradationin shake flasks.Of the five, four showed complete degradation of feather barbules within 120 h and one isolate showed degradation within 72 h. The isolate was identified morphologically and by 16 s rRNA sequencing as Breuvendimonosterrae. It produced maximum keratinase at 37 C temperature and at 130 rpm speed within 72 h. Breuvendimonosterrae showed 76U/ml enzyme activity.The bacterium can be used for biotechnological purpose. <#LINE#> @ @ Yammura S., Y. Morita, Q. Hasan, K. Yokoyama and E. Tamiya, Keratin degradation a Cooperative action of two enzymes from Stenotrophomonossp. Biochem, Biophys. Res. Commun,294, 1138-1143 (2002) @No $ @ @ Onifade A.A., N.A. Al-Sane, A.A. Al-Mussallan and S. Alzarban, Potentials for biotechnological applications of keratin degrading microorganisms and their enzymesforNutritional improvements of feathers and other keratins as livestock feed resources, Bioresource. Technol,66, 1-11 (1998) @No $ @ @ Wang J.J. and Shih J.C.H., Fermentation production of keratinase from Bacillus licheniformis PED-1 and Bacillus subtilis FDB-29, J.Ind. Microbiol Biotechnol,22, 608-619 (1999) @No $ @ @ Takami, H.H. Satoshi, R. Aono and K. Horikoshi, Degradation of human hair by a thermostable alkaline Protease from alkalophilic Bacillus sp, no.AH-101, Biosci.Biotechnol.Biochem,56, 1667-1669 (1992) @No $ @ @ Ession J.P., A.A. Umoh, E.J. Akpan, S.J. Eduok and A. Umoiyoho, Keratinolytic Proteinase activity and thermo tolerance of dermatophytes associated with Alopecia in Uyo, Nigeria, Acta Microbiological Et Immunologica Hungarica, 56, 69 (2009) @No $ @ @ Kaul S. and Sumbali G.G., Keratinolysis by Poultry farm Soil Fungi, Mycopathologia 139, 137-140 (1557) @No $ @ @ Cai C., B. Lou and X. Zheng, Keratinase Production and Keratin degradation by Mutant Strain of Bacillus subtilis, J. Zhejiang Univ.ScI.B., 60-67 (2008) @No $ @ @ P. Jeevana Lakshmi, Ch. M. Kumari Chitturi and V.V. Lakshmi, Efficient degradation of feather by keratinase producing Bacillus sp. International journal of Microbiology, Article ID 608321, (2013) @No $ @ @ Sreenivasa Nayaka and Vidyasagar G.M., Purification and characterization of Keratinase from Native feather degrading Streptomyces albus, International journal of Development Research, 3(8), 034-039 (2013) @No $ @ @ Sarita Agrahari and Neeraj Wadhwa, Degradation of chicken feather, a poultry waste Product by Keratinolytic Bacteria Isolated from Dumping Site at Ghazipur Poultry processing plant, International Journal of Poultry science, 9(5), 482-489 (2010) @No $ @ @ Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with the Folin phenol reagent, J. Biochem, 193(1), 265-75 (1951) @No $ @ @ K. Salvam and Vishnupriya B., Biochemical and Molecular characterization of Microbial Keratinase, International journal of Pharmacutical and Biological Archives, 3(2),267-275 (2012) @No $ @ @ Friedrich J., Gradisar H., Mandian D. and Chaumont J.P., Screening Fungi for Synthesis of Keratinolytic Enzymes, Letter in Applied Microbiology, 127-130 (1999) @No $ @ @ Vigneshwaran C., Shanmugam S. and Sathish Kumar T.,Screening and Characterization of Keratinase from Bacillus licheniformis isolated from namakkal Poultry farm, 2(4), 89-96 (2010) @No $ @ @ Bockle B.B. Galunski and R. Muller, Characterization of a Keratinolytic serine Protease from Streptomyces Pactum dsm 40530, Appl.Environ Microbiol,61, 3505-3710 (1995) @No $ @ @ Lee.C.G., Ferket.P.R. and Shih.C.H. Improvement of feather degradation by Bacterial Keratinase AS A Feed Additive, fasebJ,59, 1312 (1991) @No $ @ @ Riffel A.A. Brandelli, P. Heeb and F.Lucas, Characterization of new Keratinolytic bacterium that degrades native feather keratin, Arch, Microbial, 179, 258-265 (2003) @No $ @ @ Jeong J.H., Park K.H., Oh D.J., Hwang D.Y., Kim H.S., Lee C.Y., Son H.J., Keratinolytic enzyme-mediated biodegrdation of recalcitrant feather by a newly isolated Xanthomonos sp.P5. Polym. degrad.Stab.,95, 1969-1977 (2010b) @No $ @ @ Minghai Han, Wei Luo, Qiuy Gu and Xiaobin Yu. Isolation and characterization of Keratinolytic Proteaswe from a feather degrading bacterium Pseudomonos aeruginosa C 11, African Journal of Microbiology Research, 6(9), 2211-2221, (2012) @No $ @ @ Revathi K, Shaifali Singh, Mohd Azzem Khan and Suneetha V., A potential strain of keratinolytic Bacteria VIT RSAS2 from Katpadi and its Pharmacological Benefits, International Journal of Pharmacutical sciences Review and Research, 20(2),14, 89-92 (2013) @No <#LINE#>Protective Effect of Curcumin on Diethanolamine-Induced Toxic Effects on Human Spermatozoa: An in Vitro Study<#LINE#>Sneha@Panchal,Ramtej@Verma<#LINE#>34-39<#LINE#>8.ISCA-IRJBS-2014-020.pdf<#LINE#>Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad-380009, INDIA<#LINE#>20/1/2014<#LINE#>28/2/2014<#LINE#>Curcumin is a yellow pigment from Curcuma longa which has desirable preventive or putative therapeutic properties. In this study protective effect of curcumin on diethanolamine-induced toxicity on human spermatozoa in in vitro condition was investigated. For this study samples were collected from normal healthy donors. After liquefaction, samples were used for preparation of sperm suspension to evaluate sperm motility, sperm viability and sperm morphology. Statistical analysis was performed using analysis of variance (ANOVA) followed by Tukey’s test and the level of significance was accepted with p0.05. When sperm suspension was treated with diethanolamine (300 µg/ml) it caused significant decrease in sperm motility and sperm viability as compared to control. Treatment also caused significantly increased different kinds of sperm morphological abnormalities as compared to control. Addition of different concentrations (10-40 µg/ml) of curcumin to sperm suspension along with diethanolamine caused significant increase in sperm motility and sperm viability as compared to treated which was time-dependent as well as concentration-dependent. As compared to treated, concentration-dependent decrease in various kinds of morphological abnormalities were also observed. This findings clearly indicate that curcumin ameliorates diethanolamine-induced spermatotoxic effect on human spermatozoa. <#LINE#> @ @ Wagner P., Reassessment of Diethanolamine. (CAS Reg. No.111-42-2) United States Environmental Protection Agency, Washington, D.C. 20460.July 31 (2006) @No $ @ @ CIR Cosmetic ingredients review, Final report on the safety assessment of triethanolamine, diethanolamine, and monoethanolamine, J. Am. Coll. Toxicol,2, 183–235 @No $ @ @ CIR Cosmetic ingredients review. Final report on the safety assessment of cocamide DEA, lauramide DEA, linoleamiede DEA, and oleamide DEA, J. Am. Coll. Toxicol,5, 415–454 (1986) @No $ @ @ Soreat S. A., Stabilizing acetylsalicylic acid and its salts in solution, Fr. Demande,, 143, 609 (1973) @No $ @ @ Technology Planning and Management Corporation (TPMC), Report on carcinogens Background Document for Diethanolamine, In National Toxicology Program NIEHS, Durham, NC, NO1ES85421, P. 229 (2002) @No $ @ @ Hoffmann D., Brunnemann K.D., Rivenson A. and Hecht, S.S., N nitrosiodiethanolamine: analysis, formation in tobacco products and carcinogenicity in Syrian golden hamsters, IARC Sci. Publ.,41, 299-308 (1982) @No $ @ @ Barbee S. J., and Hartung R., The effect of diethanolamine on hepatic and renal phospholipid metabolism in the rat, Toxicol. Appl. 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H., EI-Bassyoni A.M. and EI-Domiaty M.A., Effect of Dermal Exposure of diethanolamine on the Testes and Sperms of Adult Albino Rats, Tanta Medical Sciences Journal, 2(1), 271-285 (2007) @No $ @ @ National Toxicological Programm {NTP}, NTP technical report on the toxicity Studies of Diethanolamine (CAS No 111-42-2) administered topically and in drinking water to F344/ N Rats and B6C3F1 mice, Toxic Report Series., 20, 1-D10 (1992) @No $ @ @ Kamboj V.P., Herbal Medicine, Current Science, 78(1), 35-9 (2000) @No $ @ @ Gupta L.M. and Raina R., Side effects of some medicinal plants, Current Science, 75, 897-900 (1998) @No $ @ @ Beevers S. and Huang S., Pharmacological and clinical properties of curcumin, Botanics: Targets and Therapy, , 5-18 (2011) @No $ @ @ Satoskar R.R., Shah S.J. and Shenoy S.G., Evaluation of anti-inflammatory property of curcumin (diferuloyl methane) in patients with postoperative inflammation, Int. J. Clin. Pharmcol. Ther. Toxicol., 24, 651-654 (1986) @No $ @ @ Srivastava R., Puri V., Srimal R.C. and Dhawan B.N., Effect of curcumin on platelet aggregation and vascular prostacyclin synthesis, Arzneim.-Forsch./Drug Res., 36, 715-717 (1986) @No $ @ @ Masuda T., Hidaka K., Shinohara A., Maekawa T., Takeda Y. and Yamaguchi H.J., Chemical studies on antioxidant mechanism of curcuminoid: analysis of radical reaction products from curcumin, Agric, Food Chem.,47, 71-77 (1999) @No $ @ @ Mariadason J.M., Corner G.A. and Augenlicht L.H., Genetic reprogramming in pathways of colonic cell maturationinduced by short chain fatty acids: comparison with trichostatin a, sulindac, and curcumin and implications forchemoprevention of colon cancer, Cancer, Res., 60, 4561-4572 (2000) @No $ @ @ Chan M.M., Inhibition of tumor necrosis factor by curcumin, a phytochemical, Biochemical. Pharmacol., 49, 1551-1556 (1995) @No $ @ @ Nagabhushan M., Amonkar A.J. and Bhide S.V., In vitro antimutagenicity of curcumin against environmental mutagens, Food Chem. Toxicol.,25, 545-547 (1987) @No $ @ @ Jordan W.C. and Drew C.R., Curcumin - a natural herb with anti- HIV activity, Natl. Med. Assoc.,88, 333 (1996) @No $ @ @ Khanna N.M., Turmeric - Nature’s precious gift, Curr Sci.,76(10), 1351-1356 (1999) @No $ @ @ Mathuria, N., and Verma, R. J., Curcumin ameliorates aflatoxininduced lipid peroxidation in liver, kidney and testis of mice—an in vitro study, Acta Pol Pharm,64, 413–16 (2007) @No $ @ @ Mathuria, N. and Verma, R. J., Curcumin ameliorates aflatoxininduced toxicity in mice spermatozoa, FertilSteril, 90, 775–80 (2008) @No $ @ @ Devi R., Mosheraju M. and Reddy D., Curcumin prevents chromium induced sperm characteristics in mice, IOSR Journal of Pharmacy, 2(2), 312-316 (2012) @No $ @ @ Mathuria N and Verma R. J., In vitro study on the effect of aflatoxin on human spermatozoa, ActaToxicologica, 15(1), 49-54 (2007) @No $ @ @ Panchal S. R. and Verma R. J., Spermatotoxic effect of diethanolamine: An in vitro study, Asian Pac J Reprod.,2(3), 196-200 (2013) @No $ @ @ Prasad M.R.N., Chinoy N.J. and Kadam K.M., Changes in succinic dehydrogenase levels in rat epididymis under normal and altered physiological conditions, Fertil. Steril.,23, 186-190 (1972) @No $ @ @ Gupta A. and Sarkar M., A study of adverse effect of arsenic on sperm structure and function in swiss mice, Indian J. Environ. & Ecoplan, 17(3), 445-449 (2010) @No $ @ @ Methews J. M., Garner C. E. and Matthews H. B., Metabolism, bioaccumulation and incorporation of diethanolamine into phospholipids, Chemical Research Toxicology,, 625-633 (1995) @No $ @ @ Barbee S. J. and Hartung R., Diethanolamine induced alteration of hepatic mitochondrial function and structure, Toxicol. Appl. Pharmacol.,47, 431–440 (1979) @No $ @ @ Lehman- McKeeman L. D. and Gamsky, E. A., Diethanolamine inhibits choline uptake and phosphatidylcholine synthesis in Chinese hamster ovary cells, Biochemical Biophysics Research Communication, 262, 600-604 (1999) @No $ @ @ Lehman- McKeeman L. D. and Gamsky E. A., Choline supplementation inhibits Diethanolamine induced morphological transformation in Syrian hamster embryo cells: Evidence for a carcinogenic mechanism Toxicological Sciences, 55, 303-310 (2000) @No $ @ @ Floyd R. A , Kotake Y., Hensley K., Nakae D. and Konishi Y., Reactive oxygen species in choline deficiency induced carcinogenesis and nitrone inhibition, Mol. Cell. Biochem.,234-235(1-2), 195-203 (2002) @No $ @ @ Rahimtula A.D., Bereziat J.C., Bussacchini-Griot V. and Bartsch H., Lipid peroxidation as a possible cause of ochratoxinA toxicity, Biochem. Pharmacol.,37, 4469 (1988) @No $ @ @ Makkar k., Agarwal A. and Sharma R., Oxidative stress and male infertility, India, J Med Res,139, 375-367 (2009) @No $ @ @ Agarwal A., Prabakaran S.A. and Said T.M.,– Prevention of oxidative stress injury to sperm, Journal of Andrology, 26, 654-660 (2005) @No $ @ @ Masuda T., Maekawa T., Hidaka K., Bando H., TakedaY.and Yamaguchi H., Chemical studies on antioxidant mechanisms of curcumin: analysis of oxidative coupling products from curcumin and linoleate, J Agric Food Chem,49, 2539-2547(2001) @No $ @ @ Chan W. and Wu H., Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells, Acta Pharmacologica Sinica, 27(9), 1192-1198 (2006) @No <#LINE#>Phenomic analyses of indigenous and exotic accessions of Mulberry (Morus spp.)<#LINE#>V.@Arora,M.K.@Ghosh,B.B.@Bindroo,G.@Gangopadhyay<#LINE#>40-48<#LINE#>9.ISCA-IRJBS-2014-032.pdf<#LINE#>Division of Plant Biology, Bose Institute, 93/1 APC Road, Kolkata – 700009, INDIA @ Central Sericultural Research & Training Institute, Berhampore-742101, INDIA<#LINE#>1/2/2014<#LINE#>13/3/2014<#LINE#>Broadening of gene pool vis-à-vis development of superior genotypes is a continuous process for Mulberry (Morus spp.) since the silkworm Bombyx mori L. thrives only on its foliage. The objective of the present study was to understand the interrelationship between selected indigenous / exotic genotypes of the Mulberry germplasm collection of Central Sericulture Research and Training Institute (CSR&TI), Berhampore, West Bengal, India with a phenomic approach. The association between traits was further elucidated to identify appropriate marker assisted selection strategies. The phenogram and factorial analysis of ninety four genotypes was constructed on the basis of fourteen quantitative parameters. The improved genotypes were found to be clubbed in the smallest cluster while seventy four percent exotic germplasm were grouped together in the largest cluster of phenetic tree. Correlation analysis between twenty seven parameters (fourteen quantitative with additional thirteen anatomical) revealed forty six significant correlation values, of which twenty eight percent correlations were negative. The negative correlations between traits of interest were found in opposite principal coordinates.<#LINE#> @ @ Amardev, S., Farooq, A. and Ahmed B.S., Spring rearing performance by feeding temperate mulberry variety on bivoltine hybrid NB4D2 x SH6 of silkworm, Bombyx moriL. ISCA J. Biological Sci., 1(2), 69-72 (2012) @No $ @ @ Awasthi, A.K., Nagaraja, G.M., Naik, G.V., Kanginakudru, S., Thangavelu, K. and Nagaraju, J., Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC Genet.,, 1 http://www.biomedcentral.com/1471-2156/5/1 (2004) @No $ @ @ Hou, Y.J., Mulberry breeding.Sericulture Department, Zhejiang AgricultureUniversity, Hangzhou, China, 1994)4.Khurana, P. and Checker, V.G., The advent of genomics in Mulberry and perspectives for productivity enhancement. Plant Cell Rep., 30, 825-838 (2011) @No $ @ @ Thangavelu, K., Mukherjee, P., Tikader, A., Ravindran, S., Goel, A.K., Girish Naik, V. and Sekar, S., Catalogue on Mulberry (Morus spp.) Germplasm, Central Sericultural Germplasm Resources Centre, Hosur, TN, India, , 1-236 (1997) @No $ @ @ Sarkar, A., Mulberry Breeding, Kalyani Publishers, India, 1-208 (2009) @No $ @ @ Prasad, R. and Gangopadhyay, G., Phenomic analyses of Indian and exotic accessions of Sesame, (Sesamum indicumL.), J Plant Breed. Crop Sc., 3(13), 336-352 (2011) @No $ @ @ YoganandaMurthy, V.N., Ramesh, H.L., Lokesh, G., Munirajappa and Yadav, B.R.D., Assessment of six Mulberry (Morus) germplasm varieties through moulting and bioassay parameters by using crossbreed silkworms Bombyx mori L. for commercial exploitation in Kolar district, Karnataka, India, Int. Res. J. Biological Sci., 2(9), 69-75 (2013) @No $ @ @ Gray, E., Evidence of phenotypic plasticity in mulberry Morus L.). Castanea, 55, 272-281 (1990) @No $ @ @ Vijayan, K., Awasthi, A.K., Srivastava, P.P. and Saratchandra, B., Genetic analysis of Indian mulberry genotypes through molecular markers, Hereditas, 141, 8 – 14 (2004) @No $ @ @ Venkataswarlu, M., Raje, Urs. S., Surendra, Nath, B., Shashidhar, H.E., Maheswaran, M., Veeraiah, T.M. and Sabitha, M.G., A first genetic linkage map of mulberry Morus spp.) using RAPD, ISSR, and SSR markers and pseudo testcross mapping strategy, Tree Genetics & Genomes, 3, 15–24 (2006) @No $ @ @ Kafkas, S., O¨zgen, M., Dogan, Y., O¨zcan, B., Ercisxli, S. and Sercxe, S., Molecular Characterization of Mulberry Accessions in Turkey by AFLP Markers. J Amer. Soc. Horti. Sci., 133(4), 593–597 (2008) @No $ @ @ Wani, S.A., Bhat, M.A., Malik, G.N., Kamili, A.S., Mir, M.R., Bhat, S.A., Wani, N., Razvi, S.M., Akhtar, S. and Bhat. K.A., Molecular markers and their role in mulberry improvement, Int. J Curr. Res., 4, 20-24 (2010) @No $ @ @ Perrier, X. and Jacquemoud-Collet J-P., DAR-win – 5.0 User’s Manual, 1-79 (2005) @No $ @ @ Sugimara, Y., Mori, T., Nitta, I., Kotani, E., Furusawa, T., Tatsumi, M., Kusakari, S.I., Wada, M.and Morita, Y., Calcium deposition in idioblasts of Mulberry leaves, Ann. Bot., 83, 543-550 (1999) @No $ @ @ Nitta, I., Kida, A., Fujibayashi, Y., Katayama, H. and Sugimara, Y., Calcium carbonate deposition in a cell wall sac formed in mulberry idioblasts,Protoplasma, 228, 201–208 (2006) @No $ @ @ Singhal, B.K., Baqual, M.F., Khan, M.A., Bindroo, B.B. and Dhar, A., Leaf surface scanning electron microscopy of 16 Mulberry genotypes (Morus spp.) with respect to their feeding value in Silkworm (Bombyx mori L.) rearing, Chilean J Agric. Res., 70(2), 191–198 (2010) @No $ @ @ Thul, S.T., Lal, R.K., Shasany, A.K., Darokar, M.P., Gupta, A.K., Gupta, M.M., Verma, R.K. and Khanuja, S.P.S., Estimation of phenotypic divergence in a collection of Capsicum species for yield related trait, Euphytica, 168,189–196 (2009) @No $ @ @ Banerjee, R., Roychowdhury, S., Sau, H., Das, B.K., Saha, A.K., Saratchandra, B. and Bajpai, A.K.,Phenotypic divergence in Mulberry (Morus spp.) germplasm collections, J Crop Improvement, 25,459–471 (2011) @No $ @ @ (http://www.fao.org/docrep/005/AD107E/ad107e0u.htm#TopOfPage) (2014) @No $ @ @ (http://www.fao.org/docrep/005/AD107E/ad107e0u.htm#TopOfPage) (2014) @No $ @ @(http://www.karnataka.gov.in/sericulture/English/Technologies/Mulberyvarities.aspx) (2014) @No <#LINE#>Women in Purdah: A Study of their Knowledge, Attitudes and Practices in Relation to Cancer of the Cervix<#LINE#>O.@AdisaJames,O.@MuhammedAmina,C.@EgbujoEjike,Adeniyi@David,A.@YahayaIsah<#LINE#>49-53<#LINE#>10.ISCA-IRJBS-2014-037.pdf<#LINE#>Department of Medical Laboratory science, University of Jos, Plateau State, NIGERIA @ Pathology Department, Federal Medical Centre Azare, Bauchi State NIGERIA <#LINE#>11/2/2014<#LINE#>25/3/2014<#LINE#>Correct and complete knowledge, positive attitudes/beliefs and positive behavioral practices are essential in the control of diseases including cervical cancer. This is in addition to routine screening of apparently healthy women. This study therefore seeks to look at the knowledge, attitude/beliefs and practices of women in purdah as it relates to cancer. A cross sectional survey was carried out on 120 sexually active women made up of 60 that practice purdah and 60 that do not practice purdah in Jos, Plateau State, North Central Nigeria. A structured questionnaire was administered on each subject. The questionnaire contained questions on socio-demographic characteristics of the subjects and questions regarding their knowledge, attitude, beliefs and practices as regards cervical cancer. Show that only 11 (18.3%) women in Purdah had previous knowledge of the cause of cervical cancer as against 35(58.3%) for the women who are not in purdah. Women in Purdah had a mean 68.3% positive Attitudes/beliefs as against 77.2% for the women who are not in purdah women who are not in purdah. In terms of their practices, the women in purdah had a mean positive practice of 62.66% as against 76.66% of the women who are not in purdah. There is need for specific and targeted awareness for women in purdah to remove barriers and fill-up gabs to early detection of premalignant lesion. The response from the women who are not in purdah also show that awareness of women in general is very low and could hinder control. <#LINE#> @ @ Ali S.F., Ayub S., Manzoor N.F., Azim S. and Afif M., et al. Knowledge and Awareness about Cervical Cancer and Its Prevention amongst Interns and Nursing Staff in Tertiary Care Hospitals in Karachi, Pakistan, PLoS ONE, 5(6), (2010) @No $ @ @ Stat bite: Cervical cancer mortality worldwide, J Natl Cancer Inst., 98. (2006) @No $ @ @ Redhwan Ahmed Al-Naggar, WY Low and Zaleha Md Isa, Knowledge and Barriers towards Cervical Cancer Screening Among Young Women in Malaysia. Asian Pacific J Cancer Prev, 11, 867-873 (2010) @No $ @ @ Pontin J et al., Strategies for global control of cervical cancer, International Journal of Cancer, 60(1), 1–26 (1995) @No $ @ @ Bergstrom R., Sparen P. and Adami H.O., Trends in cancer of the cervix uteri in Sweden following cytological screening, BritishJournal of Cancer, 81(1),159–166 (1999) @No $ @ @ Mahlck C.G., Jonsson H. and Lenner P., Pap smear screening and changes in cervical cancer mortality in Sweden, InternationalJournal of Gynaecology and Obstetrics, 44, 267–272 (1994) @No $ @ @ Morris M, et al., Cervical intraepithelial neoplasia and cervical cancer, Obstetrics and Gynecology Clinics of North America, 23, 347–410 (1996) @No $ @ @ Elovainio L., Nieminen P. and Miller A.B., Impact of cancer screening on women’s health. International Journal of Gynaecology andObstetrics, 58(1), 137–147 (1997) @No $ @ @ Arevian M. et al., A survey of knowledge, attitude, and practice of cervical screening among Lebanese/American women, Nursing Outlook, 45(1),16–22 (1997) @No $ @ @ Ombech Elizabeth A., Muigai Anne. W.T. and Wanzala Peter. Awareness of cervical cancer risk factors and practice of Pap smear testing among female primary school teachers in Kasarani division, Nairobi Kenya, African Journal of Health Sciences, 21, 121-132(2012) @No $ @ @ Peel K.R., Premalignant and malignant diseases of the cervix, In: Whitfield CR, ed. Dewhurst’s Textbook of Obstetrics and Gynaecology for Postgraduates, Oxford: Blackwell Science, 717-37 (1995) @No $ @ @ Gichangi P., Estambale B., Bwayo J., Rogo K., Ojwang S., Opiyo A. and Temmerman M., Knowledge and practice about cervical cancer and Pap smear testing among patients at Kenyatta National Hospital, Nairobi, Kenya, International Journal of Gynecological Cancer 136, 827–33 (2003) @No $ @ @ McFarland D.M., Cervical screening and Pap smear testing in Botswana: knowledge and perceptions, International Nursing Review, 50, 167–75 (2003) @No $ @ @ Gupta A., Kumar A. and Stewart D.E., Cervical cancer screening among South Asian women in Canada: the role of education and acculturation, Health Care Women International, 23,123–34 (2003) @No $ @ @ Ralston J.D., Taylor V.M., Yasui Y., Kuniyuki A., Jackson J.C. and Tu S.P., Knowledge of cervical cancer risk factors among Chinese immigrants in Seattle, Journal of Community Health, 28, 41–57 (2003) @No $ @ @ Scarinci I.C., Beech B.M., Kovach K.W. and Bailey T.L., An examination of sociocultural factors associated with cervical screening among low-income Latina immigrants of reproductive age, J Immigr Health, 5, 119–28 (2003) @No $ @ @ Ige-Ogunniyi A.E. and Ajadi T.O., ‘Women in Purdah: The Challenges of Open and Distance Education in Nigeria’, paper presented at the 2nd Conference and General Assembly of the African Council for Distance Education (ACDE), Lagos, 8-11 July (2008) @No $ @ @ Badawi J. Misconception about Islam, Minna: Islamic Education Trust Fund, 210 (1992) @No $ @ @ National Population Commission (2007) @No $ @ @ Okwi A.L., Othicno E., Byarugaba W., Okoth A.D., Wandabwa J and Ocaido M., Attitudes and Knowledge gaps of the Communities about Cervical carcinoma and it’s detection in four selected districts of Uganda, African Journal of Animal and Biomedical Sciences, 2(1),15-20 (2007) @No <#LINE#>Wheat Genotypes (Triticum aestivum L.) vary widely in their responses of Fertility traits to high Temperature at Anthesis<#LINE#>RamChandra@Choudhary,KishorNand@Sharma,Mithilesh@Kumar,Rajeev@Kumar<#LINE#>54-60<#LINE#>11.ISCA-IRJBS-2014-038.pdf<#LINE#>Department of Agricultural Biotechnology & Molecular Biology, Rajendra Agricultural University, Pusa, P.O. Box 848125, Bihar, INDIA @ National Agri-Food Biotechnology Institute, Mohali, P.O Box 160071, Punjab, INDIA<#LINE#>13/2/2014<#LINE#>25/3/2014<#LINE#> High temperature is a major environmental factor which limits the production and productivity of wheat in most cereals growing areas of the world. Eighteen wheat (Triticum aestivum L.) genotypes were screened for high temperature with respect to four traits under field and the polyhouse as normal and stressed conditions during winter session of 2011-12 at Pusa farm, Rajendra Agricultural University, Bihar, India. Spikelet fertility, number of grains per spike, number of effective tillers per plant and pollen sterility were measured and compared to the controls (without heat stress) and Sonalika as a check. The results showed a significant difference among all of the traits in stress and control conditions. On the basis of heat susceptibility index (HSI) the genotypes, Pusa gold, PBW 343, Raj 3765, HD 2888, F5-995 and K0583 were found relatively heat tolerant. Our results suggest that there are significant differences among genotypes that can be used in breeding for heat tolerance at pre anthesis and post anthesis stages and the development of high yielding wheat varieties. <#LINE#> @ @ Barnabas B.K. Jager and Feher A., The effect of drought and heat stress on reproductive processes in cereals, Plant, CellEnv., 31, 11-38 (2008) @No $ @ @ Mahesh K.S., Chandrashekara K.T., Rajashekar N. andJagannath S., Physiological behaviour of few Cultivars of Paddy (Oryzasativa L.) during Seed Germination and early Growth, subjecting to distillery Effluent Stress, Int. Res. J. Biological Sci., 2(9), 5-10 (2013) @No $ @ @ FAOSTAT, Food and Agriculture Organization of the United Nations, Food Outlook (2012) @No $ @ @ Fischer R.A., Physiological limitation to producing wheat in semitropical and tropical environments and possible selection criteria. In Proc. Int. Symp.Wheats for More TropicalEnvironments, 209-230. Mexico, DF, CIMMYT (1986) @No $ @ @ Rady M.M. and Gaballah M.S., Improving Barley Yield Grown Under Water Stress Conditions, Res.J.Recent Sci., 1(6), 1-6 (2012) @No $ @ @ Sikha S., Sunil P., Arti J. and Sujata B., Impact of Water-deficit and Salinity stress on Seed Germination and Seedling Growth of Capsicum annuum ‘SolanBharpur, Int. Res. J. Biological Sci., 2(8), 9-15 (2013) @No $ @ @ Indira C., Genotypic Differences in Effects of Arsenic on Growth, and Concentration of Arsenic in Rice Oryzasativa) genotypes, Res. J. Recent Sci., 2(3), 49-52 (2013) @No $ @ @ Rani C.R., Reema C., Alka S. and Singh P.K., Salt Tolerance of Sorghum bicolor Cultivars during Germination and Seedling Growth, Res. J. Recent Sci., 1(3),1-10 (2012) @No $ @ @ Lobell D.B., Sibley A. and Ortiz-Monasterio J.I., Extreme heat effects on wheat senescence in India, Nat climate change, 1-4, Macmillan publishers limited (2012) @No $ @ @ Rane J., Pannu R.K., Sohu V.S., Saini R.S., Mishra B., Shoran J., Crossa J., Vargas M. and Joshi K., Performance of yield and stability of advanced wheat cultivar under heat stress environments of the indo-gangetic plains, Crop Sci., 47, 1561-1572 (2007) @No $ @ @ Acevedo E., Nachit M. and Ortiz-Ferrara G., Effect of heat stress on wheat and possible selection tools for use in breeding for tolerance. In: D.A. Saunders (ed.), Wheat for the non-traditional warm areas; 401-421, Mexico, DF. CIMMYT (1991) @No $ @ @ Wardlaw I.F., Dawson I.A., Munibi P. and Fewster R., The tolerance of wheat to high temperatures during reproductive growth, II. Survey procedures and general response patterns, Aust. J. Agri. Res., 40, 1-13 (1989) @No $ @ @ Mian M.A., Mahmood A., Ihsan M. and Cheema N.M., Response of different wheat cultivars to post anthesis temperature stress, J Agri Res., 45, 269-277 (2007) @No $ @ @ Gibson L.R. and Paulsen G.M., Yield components of wheat grown under high temperature stress during reproductive growth, Crop Sci., 39, 1841-1846 (1999) @No $ @ @ Jalal Kamali M.R. and Duveiller E., Wheat Production and Research in Iran: A Success Story. In: International Symposium on Wheat Yield Potential: Challenges to International Wheat Breeding, Mexico, D.F. CIMMYT (2008) @No $ @ @ Wardlaw I.F. and Moncur L., The response of wheat to high temperature following anthesis: I, The rate and duration of kernel filling, Aust. J. Plant Physiol., 22, 391–397 (1995) @No $ @ @ Stone P.J., Savin R., Wardlaw I.F. and Nicolas M., The influence of recovery temperature on the effect of a brief heat shock on wheat: I, Grain growth, Aust. J. Pl. Physiol., 22, 945-954 (1995) @No $ @ @ Randall P.J. and Moss H.J., Some effects of temperature regime during grain filling on wheat quality, Aust. J. Agri. Res., 41, 603-617 (1990) @No $ @ @ Vos J., Effect of temperature and nitrogen supply on post-floral growth of wheat: measurements and simulations, Agricultural Research Reports, No. 911, PUDOC, PhD thesis, University of Wageningen, the Netherlands (1981) @No $ @ @ Hasan M. A. and Ahmad J. U., Kernel growth physiology of wheat under late planting heat stress, J Nat Sci., 33, 193-204 (2005) @No $ @ @ Suryavanshi P., Babu S., Baghel J.K. and Suryavanshi G., Impact of Climate Change on Agriculture and their Mitigation Strategies for Food Security in Agriculture: A Review, ISCA J. Biological Sci., 1(3), 72-77 (2012) @No $ @ @ Andrea M.H., Yuraima M., Dasybel P. and Hernan L., Genetic variability of Macrophominaphaseolina Affecting Sesame: phenotypic traits, RAPD markers and interaction with the Crop, Res. J. Recent. Sci., 2, 110-115 (2013) @No $ @ @ Hall A.E., Breeding for heat tolerance, Plant Breed. Rev., 10, 129–168 (1992) @No $ @ @ Dhanraj N.B., Bacterial Diversity in Sugarcane SaccharumOfficinarum) Rhizosphere of Saline Soil, Int. Res. J. Biological Sci., 2(2), 60-64 (2013) @No $ @ @ Zinnah K.M.A., Zobayer N. Sikdar S. U. Liza L. N. Chowdhury M. A. N. and Ashrafuzzaman M., In Vitro Regeneration and Screening for Salt Tolerance in Rice Oryzasativa L.), Int. Res. J. Biological Sci., 2(11), 29-36 (2013) @No $ @ @ Gupta R. Guhey A. Jadhav A. and Ahad I., Physiological boost to improve the yield of rice germplasm under different water regimes, Res. J. Sci., 2(1), 87-90 (2011) @No $ @ @ Waheed A., Ahmad H., Abbasi F.M., Hamid F.S., Shah A.H., Safi F.A. and Ali H., Pollen sterility in wide crosses derivatives of rice (Orizasativa L.), J. Mater. Env. Sci., 4(3), 404-409 (2013) @No $ @ @ Fisher R.A. and Maurer R., Drought resistance in spring wheat cultivars I. Grain yield responses, Aust. J. Agri. Res., 29, 897-912 (1978) @No $ @ @ Menshawy A.M.M., Evaluation of some early bread wheat genotypes under different sowing dates: 1 Earliness characters, Fifth plant breeding conf. (May), Egypt J. Pl. Breed., 11(1), 25-40 (2007) @No $ @ @ Abd El-Majeed S.A. Mousa A.M. and Abd El-Kareem A.A., Effect of heat stress on some agronomic traits of bread wheat (Triticum aestivum L.) genotypes under Upper Egypt conditions, Fayoum J. Agric Res and Dev., 19(1), 4-16 (2005) @No $ @ @ El-Morshidy M. A. Kheiralla K. A. Abdel-Ghani A. M. and Abd El-Kareem A. A., Stability analysis for earliness and grain yield in bread wheat, Second Plant Breeding. Conf. (October 2), AssiutUniv: 199-217 (2001) @No $ @ @ Saini H.S., Sedgley M. and Aspinall D., Developmental anatomy in wheat of male sterility induced by heat stress, water deficit or abscisic acid, Aust. J. Pl. Physiol., 11, 243-253 (1984) @No $ @ @ Jager K., Fabian A. and Barnabas B., Effect of water deficit and elevated temperature on pollen development of drought sensitive and tolerant winter wheat (Triticum aestivum L.) genotypes, Acta Biologica Szegediensis, 52(1), 67-71 (2008) @No $ @ @ Lalonde S., Beebe D. and Saini H.S., Early signs of disruption of wheat anther development associated with the induction of male sterility by meiotic-stage water deficit, Sex Pl. Repro., 10, 40-48 (1997) @No $ @ @ Dorion S., Lalonde S. and Saini H.S., Induction of male sterility in wheat by meiotic stage water deficit is preceeded by a decline in invertase activity and changes in carbohydrate metabolism in anthers, Pl. Physiol, 111, 137-145 (1996) @No $ @ @ Bruckner P.L. and Frohberg R. C., Stress tolerance and adaptation in spring wheat, Crop Sci., 27, 31–6 (1987) @No $ @ @ Clarke J.M., Smith T.T.F., McCaig T.N. and Green D.G., Growth analysis of spring wheat cultivars of varying drought resistence, Crop Sci., 24, 537-541 (1984) @No $ @ @ Al-Qtayk S.M., Performance of yield and stability of wheat genotypes under high stress environments of the central region of Saudi Arabia, JKAU: Met., Env. Arid Land Agri. Sci., 21(1), 81-92 (2010) @No $ @ @ Mahmoud A.M., Genotype × Environment interactions of some bread wheat Genotypes (Triticum aestivum L.), Aust J. Agric. Sci., 37(4), 119-138 (2006) @No $ @ @ Kheiralla K.A., Mohamed A., El-Morshidy M., Motawea H. and Saeid A.A., Performance and stability of some wheat genotypes under normal and water stress condition, Aust J. Agric. Sci., 35(2), 74-94 (2004) @No $ @ @ Sharma A. and Sharma P., Genetic and Phytochemical analysis of Cluster bean (Cyamopsistetragonaloba (L.) Taub) by RAPD and HPLC, Res.J.Recent Sci., 2(2), 1-9 (2013) @No <#LINE#>Wine Preparation from Different Fruit Substrates<#LINE#>Lata@Sneh,Tulika@Mishra,Anita@Kumari<#LINE#>61-64<#LINE#>12.ISCA-IRJBS-2014-043.pdf<#LINE#>Department of Biotechnology, University Institute of Science, Chandigarh University, Gharuan, SAS Nagar Punjab, INDIA<#LINE#>21/2/2014<#LINE#>5/4/2014<#LINE#>The present study involves the production of wine by using different fruit substrates, especially beet root and betel leaves. Two sets of wine were prepared, one with betel leaf and other one without betel leaf. Different parameters like acidity, pH, sugar content and alcoholic content were checked in both sets of wine and compared with each other.<#LINE#> @ @ Berry C.J.J., First steps in wine making. Published by G. W. Kent, Inc. 3667 Morgan Road, Ann Arbor M I 235 (2000) @No $ @ @ R. Nair and Sumitra Chanda., Antimicrobial Activity of Terminalia catappa, Manilkara zapota and Piper betel Leaf Extract, Indian J Pharm Sci. 70(3), 390–393(2008) @No $ @ @ Rahul Hajare, V. M. Darvhekar, Ashish Shewale and Vijay Patil., Evaluation of antihistaminic activity of Piper betel leaf in guinea pig, African Journal of Pharmacy and Pharmacology 5(2) ,113-117 (2011) @No $ @ @ L.S.R Arambewela, L.D.A.M Arawwawala, W,D Ratnasooriya.,Antidiabetic activities of aqueous and \ethanolic extracts of Piper betle leaves in rats. Ethnopharmacology102(2), 2395.Satyavati GV, Raina MK, Sharma M., Medicinal Plants of India. New Delhi: Indian Council of Medical Research, New Delhi, India. Vol 1 (1987) @No $ @ @ Medicinal plants by Shankar Gopal Joshi, Oxford $ IBH publishing Co. Pvt. Ltd. New Delhi, 307 (2000) @No $ @ @ Okafor N., Industrial Microbiology413 (1987) @No $ @ @ Prescott L.M., Harley J.P. and 7th Edn., Mc Graw-Hill Companies,the Americas, New York (2008) @No $ @ @ Slinkard Stacy Wine: Red for health 2(14), 10-11 (2007) @No $ @ @ Fessler H.J., Guidelines to Practical Wine making. Oregon Speciality Colins 7024 NE Glisem ST. Portland 117 (1988) @No $ @ @ Miller G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar,(1959) @No $ @ @ 2.Amerine, M. A. and M. A. Joslyn.,technology of their productionPress (1950) @No $ @ @ Rahul Hajare, V. M. Darvhekar, Ashish Shewale and Vijay Patil., Evaluation of antihistaminic activity of Piper betel African Journal of Pharmacy and (2011), L.D.A.M Arawwawala, W,D Ratnasooriya.,Antidiabetic activities of aqueous and ethanolic extracts of Piper betle leaves in rats. Journal of , 239–245(2005 Satyavati GV, Raina MK, Sharma M., Medicinal Plants of ndian Council of Medical Research, (1987) @No $ @ @ Medicinal plants by Shankar Gopal Joshi, Oxford $ IBH publishing Co. Pvt. Ltd. New Delhi, 307(2000) @No $ @ @ Industrial MicrobiologyUniversity of Ife Press and Klein D.A., Microbiology Hill Companies, Inc., 1221 Avenue of (2008) @No $ @ @ Slinkard Stacy Wine: Red for health – The Guardian (life), 2(14), 10-11 (2007) @No $ @ @ Fessler H.J., Guidelines to Practical Wine making. Oregon Speciality Colins 7024 NE Glisem ST. Portland 97213, 1-117 (1988) @No $ @ @ Miller G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem.,31, 426 (1950) @No $ @ @ Amerine, M. A. and M. A. Joslyn., Table wines: the technology of their productionUniversity of California Press (1950) @No $ @ @ Michael, P. Foods of the Gods: Part 1-Wine in Ancient http://www.touregypt.net/Egypt-magf.htm. ( 2010) @No <#LINE#>A comparative study on Glutathione S-transferase activities of the Needles of Two Pine species under Drought and Cold stresses<#LINE#>Can@Yilmaz<#LINE#>65-70<#LINE#>13.ISCA-IRJBS-2014-045.pdf<#LINE#><#LINE#>23/2/2014<#LINE#>3/4/2014<#LINE#>Total cytosolic Glutathione S-transferase (GST, EC 2.5.1.18) activities and the changes in the GSH pools of the needle samples from cold-resistant Pinus sylvestris and relatively drought resistant Pinus brutia species were screened for three seasons. Samples of spring season were accepted as reference for other two groups of sampling done in summer and winter; and, daily total precipitation, air humidity, minimum and maximum temperature values and freeze incidents were recorded. Results revealed the possible correlation between the dimensions of GSH pools and total GST activities of needle homogenates at cold and drought stresses. Those two species showed different behaviours for the same climatic conditions. <#LINE#> @ @ Gezer A., The Sylviculture of Pinus brutia in Turkey, Technical Report for CIHEAM, Paris, (1986) @No $ @ @ Labra M., Grassi F., Sgorbati S. and Ferrari C., Distribution of genetic variability in southern populations of Scots pine (Pinus sylvestris L.) from the Alps to the Apennines, Flora, 201(6), 468-476 (2006) @No $ @ @ Sutinena M.L., Repob T., Sutinenc S., Lasarovd H., Alvilad L. and Pakkanen T.T., Physiological changes in Pinus sylvestris needles during early spring under sub-arctic conditions, Forest. Ecol. Manag., 135(1-3), 217-228 (2000) @No $ @ @ Marrs K.A., The functions and regulation of Glutathione S-transferases in plants, Annu. Rev. Plant Physiol. Plant Mol. Biol., 47, 127-158 (1996) @No $ @ @ Seppanen M., Cardi T., Hyökki B.M. and Pehu E., Characterization and expression of cold-induced glutathione S-transferase in freezing tolerant Solanum commersonii, sensitive S. tuberosum and their interspecific somatic hybrids, Plant Sci., 153(2), 125-133 (2000) @No $ @ @ Warade W.N., Levels of Glutathione S-Transferasein Different Larval Tissues of Papilio Demoleus, Res. J. Recent. Sci., 1(ISC-2011) , 313-316 (2012) @No $ @ @ Bose P. and Bathri R., Glutathione S-Transferase gene polymorphisms (GSTT1, GSTM1, GSTP1) as increased risk factors for asthma and COPD among Isocyanate exposed population of Bhopal, India, Res. J. Recent. Sci., 1(ISC-2011), 219-223 (2012) @No $ @ @ Yadav S.K., Cold stress tolerance mechanisms in plants, A review, Agron. Sustain. Dev., 30(3), 515-527 (2010) @No $ @ @ Galant A., Preuss M.L., Cameron J.C. and Joseph M.J., Plant glutathione biosynthesis: diversity in biochemical regulation and reaction products, Front. Plant Sci., , 45 (2011) @No $ @ @ Schröder P. and Berkau C., Characterization of cytosolic glutathione S-transferase in spruce needles, Botanica Acta, 106(4), 301-306 (1993) @No $ @ @ Lowry O., Rosebrough N., Farr A. and Randall R., Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193(1), 265-275 (1951) @No $ @ @ Grill D., Pfeifhofer H. and Esterbauer H., Further investigations on the thiol content of Norway spruce needles, Phyton (Austria), 27(2), 311-317 (1987) @No $ @ @ Pukacka S. and Pukacki P.M., Seasonal changes in antioxidant level of Scots pine (Pinus sylvestris L.) needles exposed to industrial pollution, I. Ascorbate and thiol content, Acta Physiol. Plant, 22(4), 451-456 (2000) @No $ @ @ Sedlak J. and Lindsay R., Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent, Anal. Biochem., 25(1), 192-205 (1968) @No $ @ @ Habig W., Pabst M., Jakoby W., Glutathione S-transferases. The first enzymatic step in mercapturic acid formation, J. Biol. Chem., 249(22), 7130-7139 (1974) @No $ @ @ Noctor G., Mhamdi A., Chaouch S., Han Y., Neukermans J. and Garcia B.M., Glutathione in plants: an integrated overview, Plant Cell Environ., 35(2), 454-484 (2012) @No $ @ @ Kawamura Y. and Uemura M., Mass spectrometric approach for identifying putative plasma membrane proteins of Arabidopsis leaves associated with cold acclimation, Plant J., 36, 141-154 (2003) @No $ @ @ Kosmala A., Bocian A., Rapacz M., Jurczyk B. and Zwierzykowski Z., Identification of leaf proteins differentially accumulated during cold acclimation between Festuca pratensis plants with distinct levels of frost tolerance, J. Exp. Bot., 60, 3595-3609 (2009) @No $ @ @ Manoj K. and Padhy P.K., Oxidative Stress and Heavy Metals: An Appraisal with Reference to Environmental Biology, Int. Res. J. Biological Sci.,2(10), 91-101 (2013) @No $ @ @ Davis D.G. and Swanson H.R., Activity of stress-related enzymes in the perennial weed leafy spurge (Euphorbia esula L.), Environ. Exp. Bot., 46, 95-108 (2001) @No $ @ @ Hausladen A. and Alscher R.G., Cold-Hardiness-Specific Glutathione Reductase lsozymes in Red Spruce-Thermal Dependence of Kinetic Parameters and Possible Regulatory Mechanisms, Plant Physiol., 105, 215-223 (1994) @No $ @ @ Rigling, A., Brühlhart, H., Bräker, O. U., Forater, T., Schweingruber, F. H., Effects of irrigation on diameter growth and vertical resin duct production in Pinus sylvestris L. on dry sites in the central Alps, Switzerland, Forest Ecol. Manag.,175 (1-3), 285-296,(2003) @No $ @ @ Gruber A., Pirkebner D., Florian C. and Oberhuber W., No evidence for depletion of carbohydrate pools in Scots pine Pinus sylvestris L.) under drought stress, Plant Biol., 14 (1), 142-148 (2012) @No $ @ @ Bigler C., Bräker O., Bugmann H., Dobbertin M., Rigling A., Drought as an inciting mortality factor in Scots pine stands of the Valais, Switzerland, Ecosystems, , 330-343 (2006) @No <#LINE#>A Preliminary Systematic study of Spiders of Major Wetlands of Anand-Kheda districts, Gujarat, India<#LINE#>Nikunj@Bhatt<#LINE#>71-73<#LINE#>14.ISCA-IRJBS-2014-151.pdf<#LINE#> V.P. and R.P.T.P. Science College,Vallabh Vidyanagar, Gujarat, INDIA<#LINE#>31/5/2014<#LINE#>30/6/2014<#LINE#>Major wetlands like Pariej and Kanewal are the prime habitats for birds and aquatic fauna but during the primary study of these wetlands forty species of Spiders belonging to twenty six different genera distributed in sixteen different families are enlisted here from these two wetlands of Anand-kheda districts of Gujarat, out of which four species from three genera are being recorded again after such a long span from Gujarat State. <#LINE#> @ @ Tikader B.K., Fauna of India, Araneae (Thomisidae & Lycosidae), Rec. zool. Surv. 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Among all categories of seeds, discoloured seeds of Pigeon pea showed maximum seed mycoflora. <#LINE#> @ @ Shakuntala Manay, N. and M. Shadaksharaswamy: Foods: Facts and principals, Wiley eastern limited (1987) @No $ @ @ Neergaard, Paul: Seed pathology John Villy and sons, N.Y. , (1977) @No $ @ @ Subramanian C.V.: Hypomycetes: An account of Indian species, Except Cercospora, ICAR, New Delhi, 930 (1971) @No $ @ @ Neergaard P. and S.B. Mathur: University teaching of seed pathology, published by Prasaranga, University of Mysore, India (1980) @No $ @ @ Jha D.K.: A text book of seed pathology. Vikas publishing house pvt. Ltd. New Delhi, (reprint 1995, 132) (1993) @No $ @ @ Mukadam, D.S.: The illustrated kingdom of fungi (some selected genera). Published by Akshar Ganga prakashan, Aurangabad, India (1997) @No