International Research Journal of Biological Sciences ___________________________________ ISSN 2278-3202Vol. 2(1), 64-66, January (2013) Int. Res. J. Biological Sci. International Science Congress Association 64 Short Communication The Growth Performance of Alangium lamarckii as affected byvarious level of IBAAhirwar J.R. Department of Botany, Govt. College Niwari, Distt. Tikamgarh, MP, INDIAAvailable online at: www.isca.in Received 10th November 2012, revised 16th November 2012, accepted 4th December 2012Abstract The present study reports the growth performance of seedlings of Alangium lamarckii as affected by different concentrations of IBA. The results revealed that the maximum growth (77.93cm) and biomass accumulation (3.175gm) of seedlings were found in 100 ppm, followed by 50ppm and 25 ppm of IBA as compared to control. It is clear that all treatments of IBA concentrations were effective but 100 ppm level of IBA was more effective than the lower concentrations of IBA. Keywords: Growth, biomass production, Alangium lamarckii, IBA, concentration. Introduction Alangium lamarckii Thwaites isgenerally known as Akola belongs to the Family Alangiaceae (Old Family-Cornaceae). It is a small deciduous rambling shrub or small moderate tree with grey bark. Normally it attains the height about 3-10 meter and girth up to 0.50 meter which grows in the greater parts of India. It is found in the forest of Jhansi district of Uttar Pradesh, Tikamgarh and Chhatarpur districts of Madhya Pradesh. Akola is a very important medicinal plant and being used as indigenous drugs by the people of village community. Plant growth regulators are the chemical which enhance the plant growth when applied in very minute quantity. Exogenous plant growth regulators are one of the most commonly used methods. (Polat and Kamiloglu, 2007).The application of certain plant growth regulators enhance the biomass production expressed as fresh weight and dry weight. Plant growth regulators are being increasingly used as an aid to enhance yield and quality of the crops. The most of the physiological activities of plant become regulated by nutritional and genetic factors. In addition of nutritional and genetic factors, growth and development of plants depend on some growth hormones which regulate the distribution of nutrients in the different organs of plants. Plant growth regulators are well known for enhancing the growth, development and biomass production of plants. The foliar sprays of growth hormones have significant values for enhancing the quality of seedlings. The present study was aimed to see the effect of IBA concentrations on growth and biomass production of seedlings of Alangium lamarckii. Material and MethodsTo study the effect of IBA on seedlings growth of Alangium lamarckii, the aqueous solution of IBA at three different concentrations viz. IBA 25ppm-(H1), IBA 50ppm-(H2), IBA 100ppm-(H3), were prepared separately. The desired quantities of IBA were weighed with the help of electronic balance. Thereafter IBA was dissolve in absolute alcohol and then water was added in required quantity and few drops of ammonium hydroxide were also added to dissolve the IBA completely. These solutions were applied separately on one month of old seedlings of Alangium lamarckii. Plants under control (C) were treated with water in the same way as treated with IBA. Four replicates were used for each treatment. These sets were placed at nursery in open sunlight. A total 12 foliar sprays of different concentration of IBA were exogenously applied with the help of hand sprayer at the interval of 10 days. Results and DiscussionThe resultsof seedling growth of Alangium lamarckii as affected by different concentrations of IBA are presented in table-1 and figure-1. The average maximum numbers of leaves/seedling (16.0) were recorded in 100ppm followed by 50ppm (14.5) and 25ppm (13.1). Whereas, average minimum leaves were recorded in control (11.25/seedling). The average maximum dry weight of leaves (0.480gm) was noted in 100ppm followed by 0.372gm in 50ppm and 0.272gm in 25ppm as compared to control (0.158gm). Thus, the gradual increase was recorded with an increase in concentration of IBA. The average maximum amount of root moisture content (71.75%) shoot moisture content (69.0%) and plant moisture content (73.44%) were recorded in control. The average minimum moisture content of same was recorded in 100ppm which gradually increased with decreased concentrations of IBA i.e. 50ppm and 25ppm respectively. Thus a trend of gradual decrease in moisture content was found with an increase in concentration of IBA. International Research Journal of Biological Sciences ________________________________________________ ISSN 2278-3202 Vol. 2(1), 64-66, January (2013) Int. Res. J. Biological Sci. International Science Congress Association 65 The maximum amount of leaves moisture content (81.5%) was observed in control followed by 78.68 % in 50 ppm and 70.74 % in 100ppm concentration of IBA with minimum 69.65% in 25ppm. Thus the moisture content does not show any trend with IBA treatment as found in case of root, shoot and plant. Table-1 Growth and biomass production of Alangium lamarckii as affected by various level of IBA Sr. Various measurement of seedling Control (C) IBA Concentration 25ppm(H-1) 50ppm(H-2) 100ppm (H-3) 01 Number of leaves 11.25±1.708 13.00±2.160 14.5±3.109 16.00±2.828 02 Root length(cm) 29.75±1.708 30.33±1.247 33.18±3.139 39.55±2.068 03 Shoot length(cm) 27.15±3.320 28.25±1.287 28.93±1.864 38.38±2.724 04 Seedling length(cm) 56.9±4.997 58.58±2.384 62.1±4.134 77.93±4.774 05 Shoot/ Root length ratio 0.910±0.063 0.932±0.029 0.877±0.087 0.970±0.019 06 Leaves moisture content (%) 81.5±2.082 69.65±0.674 78.68±1.515 75.74±0.965 07 Root moisture content (%) 71.75±2.062 69.94±1.822 68.77±1.492 67.25±2.058 08 Shoot moisture content (%) 69.00±1.155 68.68±1.631 67.71±1.474 66.23±0.515 09 Total moisture content (%) 73.44±1.547 72.35±0.887 70.98±1.590 68.51±1.295 10 Leaves dry weight (gm) 0.158±0.020 0.272±.0.040 0.372±0.025 0.480±0.016 11 Root dry weight (gm) 0.458±0.023 1.032±0.078 1.366±0.206 1.881±0.114 12 Shoot dry weight (gm) 0.292±0.060 0.397±0.024 0.531±0.052 0.815±0.022 13 Seedling dry weight (gm) 0.908±0.012 1.700±0.132 2.270±0.210 3.175±0.126 14 Shoot/Root dry weight ratio 0.639±0.019 0.385±0.019 0.541±0.055 0.434±0.026 15 Collar circumference 0.33±0.008 0.38±0.013 0.42±0.012 0.43±0.008 Figure-1 Growth and biomass production of Alangium lamarckii as affected by various level of IBA 102030405060708090H-1H-2H-3CONTROLVARIOUS LEVEL OF IBA AS PER TABLE-1 GROWTH BIOMASS International Research Journal of Biological Sciences ________________________________________________ ISSN 2278-3202 Vol. 2(1), 64-66, January (2013) Int. Res. J. Biological Sci. International Science Congress Association 66 The average maximum length of shoot (38.38cm), root (39.55cm) and seedling (77.93cm) were noticed in 100ppm concentration of IBA followed by 50ppm and 25ppm of IBA. These values were found minimum in case of control. The dry weight of shoot (0.815gm), root (1.881gm) and seedlings (3.175gm) were found maximum in 100ppm followed by 50ppm and 25ppm of IBA. The minimum values were recorded in control. A trend of gradual increase in these parameters of seedling growth was found with an increase in the concentration of IBA. The average maximum shoot/root ratio (0.970) was found when plant treated with 100ppm of IBA and the average minimum (0.877) was recorded in 50ppm followed by 0.932 in 25ppm of IBA as compared to control (0.910). The average maximum shoot/root dry weight ratio (0.639) was noted in control. The average minimum (0.385) was in 25ppm followed by 0.434 in 100ppm and 0.541 in 50ppm of IBA. No gradual increase was observed with an increase in the concentration of IBA. The average maximum collar circumference (0.43) was observed in 100ppm followed by 0.42 in 50ppm and 0.38 in 25ppm as compared to control (0.38). Thus a trend of gradual increase was observed in collar circumferences with an increase of IBA concentration. It is clear from above results that the 100 ppm was more effective than the lower concentrations of IBA. These findings are in agreement with those who observed that the length of cutting planted seedlings of betel vine significantly increased with 100ppm of IBA concentration. The maximum plant height was observed in 200ppm of IBA concentration. IBA (0.01-0.05ppm) enhanced the plant height and dry weight in S. siamiaand plant height only in P. biglobosa, while 0.03-0.04ppm of IBA enhanced the number of leaf in P. biglobosa and A. lebbekseedlings. All growth regulators (IBA, IAA and NAA) were found to be statistically significant. The beneficial effect of different levels of plant growth regulators may be due to the stimulation of hydrolysis of nutritional reserves and their mobilization in the region of root formation10. ConclusionBased upon the above results it can be concluded that the growth of seedlings of Alangium lamarckii was significant in all treatments of IBA concentrations as compared to control, but, they were expressing a very luxuriant growth when treated with 100 ppm of IBA. A trend of gradual increase of seedling growth was also found with an increase in the concentration of IBA. It is clear that the higher growth performance and dry weight accumulation of seedling of Alangium lamarckii can be achieved by spraying 100ppm of IBA as compared to lower concentration. References1.Ahirwar J.R. and Tripathi J.P., A new report on occurrence of polyembryony in Alangium lamarckii Thw. Vaniki Sandesh, 1(1), 1-3 (2010) 2.Naeem M., Iram Bhatti, Raza Hafeez Ahmad and Yasin Ashraf M.,Effect of some growth hormones (GA3, IAA and kinetin) on the morphology and early or delayedinitiation of bud of lentil (Lens culinaris Medik) Pak. J. Bot., 36 (4), 801-809 (2004) 3.Polat A.A., Durgaç C., Kamilo lu Ö., The Effects of Indole butyric acid (IBA) on rooting of fig cuttings (in Turkish with English Abstract), J. Agr Sci., 5(1-2), 1-6 (2000) 4.Vamil Rashmi, Aniat-ul-haq, R.K. Agnihotri and Rajendra Sharma, Effect of certain plant growth regulators on the seedling survival, biomass production and proline content of Bambusa arundinacea, Science Research Reporter,1(2), 44-48 (2011) 5.Nickell L.G., Plant growth regulators: Agricultural uses, Springer Verlag, Berlin, (1982)6.Sengupta S.K., Bhatt Jayant and Chaurasia R.K., Effect of hormones in inducing roots in Betelvine Piper betelL.), Advances of Plant sciences, 10 (2), 267-269 (1997) 7.Sigh, Aradhana, Mishra U.S., singh Yashpal and Paroha Seema, Effect of foliar applied phytohormones on the vegetative growth of Kalmegh (Andrographis paniculata), Journal of Tropical Forestry, 27 (1), 38-42 (2011) 8.Ebofin, A.O., Agboola D.A., Aduradola A.M. and Ayodele M.S. Effect of some growth hormones on seed germination and seedling growth of some savanna tree legumes, The Indian Forester, 130 (11), 1241-1249 (2004) 9.Chand, Gopi and Sood Anil, The influence of some growth regulators on the seed germination of Dendrocalamus strictus Nees, The Indian Forester, 134 (3), 397-402 (2008)10.Zahoor Ahmad Sofi, Kukadia M.U. and Nayak D., Effect of plant growth regulators on rooting and sprouting behaviour of cuttings of derris indica, Journal of Tropical Forestry 27(3), 32-35 (2011)