@Research Paper
<#LINE#>Ultrastructural Effects of Titanium Dioxide on Epithelial Cells of Small Intestine of Mice<#LINE#>Melekoglu@Abdullah,Turan@Guven,Turker@Huseyin<#LINE#>1-7<#LINE#>1.ISCA-IRJBS-2012-241.pdf<#LINE#> kkale University, Faculty of Science, Department of Biology, 71450, Yahsihan,kkale, TURKEY @ Gazi University, Gazi Education Faculty, Department of Biology, 06500, Teknikokullar, Ankara, TURKEY @ Ankara University, Science Faculty, Department of Biology, 06500, Tandogan, Ankara, TURKEY <#LINE#>28/12/2012<#LINE#>9/1/2013<#LINE#>In this study, the effects of different doses of Ti02 (E171) on the mucosa cells of small intestine, orally administered to Swiss albino mice (Mus musculus domesticus) have been investigated. After administration of Ti02 to mice, the changes occurred in the organelles such as nucleus, mitochondria and endoplasmic reticulum. The most prominent changes in the mucosal cells were dilation in the endoplasmic reticulum, nuclear space formation and disappeared matrix and cristae in mitochondria. Furthermore, lipid droplets were found in cytoplasm. <#LINE#> @ @ FAO/WHO, Evaluation of certain food additives and contaminants. Report of the joint FAO/WHO expert committee on food additives, WHO Technical Report Series806 (1991) @No $ @ @ Gurr J.R., Wang A.S., Chen C.H. and Jan K.Y., Ultrafine titanium dioxide particles in the absence of photo activation can induce oxidative damage to human bronchial epithelial cells, Toxicol., 213(1-2), 66-73 (2005) @No $ @ @ Hallagan J. B., Ailen D.C and Borzelleca J.F., The Safety and Regulatory Status of Food, Drug and Cosmetics Color Additives Exempt from Certification, Food and Chemical Toxicol., 33(6), 515-528 (1995) @No $ @ @ Sahrul S., Rita P., Marina I.H., Pepen A., Khairurrijal and Mikrajuddin A., Efficiency Improvement in Tio2-Particle Based Solar Cells After Deposition of Metal in Spaces Between Particles, Int. J. Basic & Applied Sc.,  11, 15-28  (2011) @No $ @ @ JECFA, Joint FAO/WHO Expert Committee on Food Additives. Titanium dioxide in combined compendium of Food Additive Specifications, FAO, Roma., 3 (2006) @No $ @ @ Mano S.S., Kanehira K.,Sonezaki S. and Taniguchi A., Effect of Polyethylene Glycol Modification of TiONanoparticles on Cytotoxicity and Gene Expressions in Human Cell Lines, Int. J. Mol. Sci.,13(3), 3703-3717 (2012) @No $ @ @ Sungkaworn T., Triampo W., Nalakarn P., Triampo D., Tang I. M., Lenbury Y. and Picha P., The Effects of TiO2 Nanoparticles on Tumor Cell Colonies: Fractal Dimension and Morphological Properties, Int. J. Biol. Life Sci., 2(1), 67-74 (2006) @No $ @ @ AL-Shinnawy M., Physiological effect of a food additive on some hematological and biochemical parameters of male albino rats, Egypt. Acad. J. Biol. Sci.,2(1), 143-151 (2009) @No $ @ @ Boffetta P., Bioassay of Ti0 for possible carcinogenicity (CAS No: 13463 - 67 - 7),  Int. J. Pharm. 107(3), 117-128 (1995) @No $ @ @ Afaq F., Abidi P., Matin R. and Rahman Q., Cytotoxicity, pro-oxidant effects and antioxidant depletion in rat lung alveolar macrophages exposed to ultrafine titanium dioxide, J. Appl. Toxicol., 18(5) 307–312 (1998) @No $ @ @ . Chen, E., Ruvalcaba, M., Araujo, L., Chapman, R. and Chin, W.C., Ultrafine titanium dioxide nanoparticles induce cell death in human bronchial epithelial cells. . Exp. Nanosci. , 171–183 (2008) @No $ @ @ Sycheva L.P., Zhurkov V.S., Iurchenko W., Daugel-Dauge N.O., Kovalenko M.A., Krivtsova E.K. and Durnev A.D., Investigation of genotoxic and cytotoxic effects of micro and nanosized titanium dioxide in six organs of mice in vivo, Mut. Res., 726(1), 8-14 (2011) @No $ @ @ Huang K,. Chen L., Liao M. and Xiong J., The Photocatalytic Inactivation Effect of Fe-Doped TiO2 Nanocomposites on Leukemic HL60 Cells-Based Photodynamic Therapy, Int. J. Phot., 1-8) (2012) @No $ @ @ Yang H., Qin X., Tian A. Zhang D., Xue X. and Wu A., Nano Size Effects of TiO2 Nanotube Array on the Glioma Cells Behavior,  Int. J. Mol. Sci., 14, 244-254 (2013) @No $ @ @ Nabela, I., EL- Sharkawy, Salah, M. Hamza and Ehsan, H., Abou-Zeid,  Toxic Impact of Titanium Dioxide (TiO2) In Male Albino Rats with Special Reference to its Effect on Reproductive System, J. Am. Sci., 6(11), 865-872 (2010) @No $ @ @ Iavicou I., Leso V., Fontana L., and Bergamaschi A., Toxicological effects of Titanium dioxide nanoparticles: a review of in vitro mammalian studies, Eur. Rev. Med. Pharm. Sci., 15, 481-508 (2011) @No $ @ @ Iavicou I, Leso V. and Bergamaschi A., Tocxicological effects of Titanium Dioxide Nanoparticles: A Review of In Vivo Studies, J. Nanomaarials, 36 (2012) @No $ @ @ Zeng L., Zheng Z. and Zhang S., Pathogenic effects of Ti02 dust on the lung of dogs, Med. Sci., 20(1), 88-91 (1989) @No $ @ @ Cesar A.M., Florabel G., Mullick M.D., Kamal G., Ishak M.D., Frank B., Johnson M.D.,  William B. and Hummer B.S., Identification of Titanium in Human Tissues: Probable Role in Pathologic Processes, Hum. Pathol.22(5), 450-454 (1991) @No $ @ @ Donaldson K., Brown M.G., Brown D.M., Robertson D.M., Slight J., Cowie H., Jones A.D., Bolton R.E. and Davis J.M.G., Contrasting Bronchoalveolar Leukocyte Responses in Rats Inhaling Coal Mine Duts, Quartz, or Titanium Dioxide: Effects of Coal Rank, Airborne Mass Concentration and Cessation of Exposure, Envir. Res., 52, 62-76 (1990) @No $ @ @ Rosenberg K.W., Gratz H.H. and Sailer F., Should titanium manipulates be removed after bone healing is complete?Int. J. Oral Max. Surg., 22, 185-188 (1983) @No $ @ @ Nicola I., Stefanelli G., Valletrisco M. and Amato P., Effects of a diet containing titanium dioxide on albino rats, Industrie-Alimentari., 16(1), 88-92 (1977) @No $ @ @ Praful U.J., Mc Carthy D.E. and Alexander T., Titanium dioxide (rutile) particle uptake from the rat Gl tract and translocation to systemic organs after oral administration, Int. J. Pharm., 105(2), 157-168 (1994) @No $ @ @ Driscoll K.E., Lindenschmidt R.C., Maurer J.K., Perkins L., Perkins M. and Higgins J., Pulmonary response to inhaled silica or titanium dioxide, Toxicol. and App. Pharm., 111(2), 201–210 (1991) @No $ @ @ McMillan H., Jones A.D., Vincent J.H., Johnston A.M., Douglas A.N. and Cowie H., Accumulation of Mixed Mineral Dust in the Lungs of Rats During Chronic Inhalation Exposure, Envir. Res., 48, 218-237 (1988) @No $ @ @ Schilling K., Bradford B., Castelli D., Dufour E., Nash J.F., Pape W., Schulte S., Tooley I., van den Bosch J. and Schellauf F., Human safety review of "nano" titanium dioxide and zinc oxide, Photochem. Photobiol. Sci., 9(4), 495-509 (2010) @No 
<#LINE#>Describing Variability in Mandible Shapes in Selected Workers of the Ant Diacamma rugosum (LeGuillou) 1842 (Hymenoptera: Formicidae: Ponerinae)<#LINE#>Manting@MuhminMichaelE.,J.Torres@MarkAnthony,Demayo@CesarG.<#LINE#>8-15<#LINE#>2.ISCA-IRJBS-2013-013.pdf<#LINE#> Department of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City, PHILIPPINES<#LINE#>23/1/2013<#LINE#>26/2/2013<#LINE#>This study was conducted to determine mandibular shape variation in Diacamma rugosum which still retains the primitive faculties as solitary hunter and as a social worker. The specimens used in this study were collected from Initao National Park, Initao, Misamis Oriental and stored in 70% ethyl alcohol solution. Body size index measurements of each worker were taken. The mandibles of the ants were dissected from 60 randomly selected worker ants and photographed prior to Geometric Morphometric analyses. A total of 100 points were digitized from images of the mandibles using TpsDig ver. 2.12. The X and Y coordinates of the outline points were saved in Matlab format and were subjected to Relative Warp Analysis to remove non-shape components. Results showed that size-dependent shape variation was not observed in the solitary species D. rugosum. Pearson correlation values for shape variables against body size in D. rugosum ranged only from r = 0.006 to 0.197. These results show that age- and size- related changes in the shapes of the mandible may accompany task partitioning in ants and may be important in studying the evolution of sociogenesis in ants.<#LINE#> @ @ Gotward Jr. W.H., Comparative Morphological Studies of the Ants, with Particular Reference to the Mouthparts (Hymenoptera: Formicidae), Cornell University Agricultural Experiment Station, New York State College of Agriculture, Ithaca, N.Y. (1969) @No $ @ @ Wilson E.O., The Insect Societies, Cambridge, MA: Harvard Univ. Press, 548 (1971) @No $ @ @ Hölldobler  B. and Wilson E.O., The Ants, Harvard: Belknap Press of Harvard Univ. Press (1990) @No $ @ @ Higashi S., Worker Polyethism Related with Body Size in a Polydomous Red Wood Ant, Formica (Formica) yessenis, Journal of theFaculty of Science Hokkaido University Series VI. Zoology, 19(3), 695-705 (1974) @No $ @ @ Traniello J.F.A., Population structure and social organization in the primitive an Amblyopone pallipes (Hymenoptera: Formicidae), Psyche, 89, 65–80 (1982) @No $ @ @ Hart A.G. and Ratnieks F.L.W., Task partitioning, division of labour and nest compartmentalisation collectively isolates hazardous waste in leaf cutting ant Atta cephalotes, Behav Ecol Sociobiol, 49, 387-392 (2001) @No $ @ @ Theraulaz G., Gervet J., Thon B., Pratte M. and Semenoff-Tian-Chansky S., The dynamics of colony organization in the primitively eusocial wasp Polistes dominulus (C.), Ethology,  91, 177 –202  (2002) @No $ @ @ da Silva-Melo A. and Giannnotti E., Division of labor in Pachycondyla striata Fr. Smith, 1858 (Hymenoptera: Formicidae: Ponerinae) Psyche, Vol. 2012  Hindawi Publishing Corporation,  p7 (2012) @No $ @ @ Jeanne R.L., The evolution of the organization of work in social insects, Monitor Zool. Ital., 20, 119–134 (1986) @No $ @ @ Ratnieks F.L.W. and Anderson C., Task partitioning in insect societies, Insectes Soc., 46, 95–108 (1999) @No $ @ @ Rohlf F.J. and Marcus L.F., A revolution in morphometrics, Trends Ecol. Evol.,  8, 129-132 (1993) @No $ @ @ Adams D.C., Rohlf F.J., and Slice D.E., Geometric Morphometrics: Ten Years of Progress following the Revolution, In A. Loy,M. D.C. Corti, D.C. Adams, D.E. Slice and F.J. Rohlf. (eds), Proceeding of Rome Geometric Morphometrics Workshop, Homepage to Leslie F. Marcus – Il,  J. Zool., 71, 5-10 (2004) @No $ @ @ Marcus L.F., Corti M., Loy A., Naylor G.J.P., Slice D., (Eds.), Advances in Morphometrics, NATO ASI Series A: Life Sciences, vol. 284, New York, Plenum Press, (1996) @No $ @ @ Kendall D.G., The statistics of shape, In: Barnett, V. (ed). Interpreting multivariate data, 75-80, Wiley, New York, 1981) @No $ @ @ Kendall D.G., Shape-manifolds, Procrustean metrics and complex projective spaces, Bull.Lond. Math. Soc., 16, 81-121 (1984) @No $ @ @ Goodall C.R.  and Bose A., Procrustes techniques for the analysis of shape and shape change,  In: Heiberger, R. (ed). Computer science and statistics: Proceedings of the 19th symposium on the interface, 86-92 Alexandria, Virginia: Amer. Stat. Assn, (1987) @No $ @ @ Chapman R.E., Conventional Procrustes approaches,  In: Rohlf F.J. and Bookstein F.L. (eds), Proceedings of the Michigan Morphometrics Workshop, 251-267, Museum of Zoology special publication no. 2, University of Michigan, Ann Arbor (1990) @No $ @ @ Rohlf F.J. The analysis of shape variation using ordinations of fitted functions, In: Sorensen J.T. and Foottit. R. (eds), Ordinations in the study of morphology, evolution and systematics of insects: applications and quantitative genetic rationales, 418, Elsevier, Amsterdam (1990) @No $ @ @ Goodall C.R.,  Procrustes methods in the statistical analysis of shape, J. R. Stat. Soc., B53, 285-339 (1991) @No $ @ @ Marcus L.F., Bello E. and Garica-Valdecasas A., (Eds.), Contributions to Morphometrics, Madrid, Spain, Museo Nacional de Ciencias Naturales (1993) @No $ @ @ Bookstein F.L. Principal warps: Thin-plate splines and the decomposition of deformations, Trans. Pat. Anal. Mach. Int., 11, 567-585 (1989) @No $ @ @ Bookstein F.L., Morphometric tools for landmark data: Geometry and Biology, New York, Cambridge University Press, 435 (1991) @No $ @ @ Rohlf F.J.  and Slice D., Extensions of the Procrustes method for the optimal superimposition of landmarks, Syst. Zool., 39, 40-59 (1990) @No $ @ @ Slice D., Geometric morphometrics Contributions to medical entomology, In Encyclopedia of Infectious Diseases, Modern Methodologies, Chapter 25, Edited by Tibayrenc M. Wiley & Sons, 435-447 B (2007) @No $ @ @ Rohlf F. J. Multivariate analysis of shape using partial-warp scores, In: Mardia, K. V. & Gill, C. A. (eds). Proceedings in current issues in statistical shape analysis. p. 154-158. Leeds Univ. Press, Leeds (1995) @No $ @ @ Rohlf F.J., 2008. tpsDig – tps Digitizer program, version 2.12. Department of Ecology and Evolution. C N.Y., State Univ. at Stony Brook (2008) @No $ @ @ Rohlf F.J., 2008. tpsRelw. tps RelativeWarp program, version 1.46. Department of Ecology and Evolution. N.Y., Software. State University at Stony Brook (2008) @No $ @ @ Hammer Ø., Harper D.A.T. and Ryan P.D., PAST: Paleontological Statistics Software package for education and data analysis. Paleotological Electronica 4(1): 9pp., Access December 2011 version 2.13. (2011) @No $ @ @ Sakagami S. F., Untersuchungen ¨uber die Arbeitsteilung in einem Zwergvolk der Honigbiene. Beitr¨age zur Biologie des Bienenvolkes, Apis mellifera L., Japanese Journal of Zoology, , 117–185 (1953) @No $ @ @ Szathm´ary E. and Maynard Smith J., The major evolutionary transitions, Nature , 374(6519) , 227–32 (1995) @No $ @ @ Gordon D.M., The organization of work in social insect colonies, Nature, 380(6570) , 121–24 (1996) @No $ @ @ Oster G.F. and Wilson E.O., Caste and Ecology in the Social Insects, Princeton, NJ: Princeton Univ. Press (1978) @No $ @ @ Detrain C., Pasteels J.M., Caste differences in behavioral thresholds as a basis for polyethism during food recruitment in the ant, Pheidole pallidula (Nyl) (Hymenoptera, Myrmicinae), J. Insect Behav. 4(2), 157–76 (1991) @No $ @ @ Robinson E.J.H., Feinerman O.  and Franks N.R, Flexible task allocation and the organization of work in ants, Proc. R. Soc. B, 276(1677) , 4373–80 (2009) @No $ @ @ Wilson E.O., Caste and division of labor in leaf-cutter ants (Hymenoptera, Formicidae, Atta). 1, The overall pattern in Atta sexdens. Behav. Ecol. Sociobiol, 7(2), 143–56 (1980) @No $ @ @ Mertl A.L. and Traniello J.F.A., Behavioral evolution in the major worker subcaste of twig-nesting Pheidole (Hymenoptera: Formicidae): Does morphological specialization influence task plasticity? Behav. Ecol.Sociobiol., 63(10), 1411–26 (2009) @No $ @ @ Wilson E.O., Between-caste aversion as a basis for division of labor in the ant Pheidole pubiventris (Hymenoptera, Formicidae), Behav. Ecol. Sociobiol17(1), 35–37 (1985) @No $ @ @ Arnan X.,  Rovira M. F., Pladevall C. and Rodrigo A., Worker size-related task partitioninf in the foraging strategy of a seed-harvesting ant species,  Iberomyrmex, No. , (2011) @No 
<#LINE#>Synthesis and Antifungal studies of Glycine and Glycine-metal complexes on Phytophthora Capsici<#LINE#>DeviG.@Indira,P.@Smitha<#LINE#>16-21<#LINE#>3.ISCA-IRJBS-2013-053.pdf<#LINE#>Department of Chemistry, Zamorin’s Guruvayurappan College, Calicut, Kerala, INDIA<#LINE#>6/3/2013<#LINE#>25/4/2013<#LINE#>Phytophthora Capsici is an important pathogenic species attack on plants. Several metal complexes are known to act as antifungal agents. Amino acid complexes of transition metals are long been used for antifungal studies. The antifungal behavior of the glycine - copper acetate complex and glycine - cobalt acetate complex has been studied individually. The effect of the above mentioned glycine complexes were tried on mycelial growth phase and sporulation phase of Phytophthora Capsici, which is a typical fungus. Foot rot caused by Phytophthora Capsici is a major production constraint in black pepper, the king of spices. The results showed that glycine copper (II) complex is a very good antifungal agent. Even in lower concentration also these amino acid metal complexes are good inhibitors against two growth phases of Phytophthora Capsici. <#LINE#> @ @ Nair N.K. and Y.R. Sarma, International pepper community work shop on joint research for control of black pepper diseases, Goa, J. of plantation crops , 27–29, 16, 2 146 - 149 (1988) @No $ @ @ Sarma, etal., Black Pepper Dis,  National Research Center for Spices, Calicut, 55-109 (1991) @No $ @ @ Sarma etal, In Proceedings of the workshop on phytophthora disease of tropical cultivated plants, (1982) @No $ @ @  Deward P.W.F., Evaluation of the results on eradication of Phytophthora foot rot of black pepper, piper nigrumm L, 1–47, (1979) @No $ @ @ Somers, E, Science progress, 50, 218, (1962) @No $ @ @ Chanmyia S., Shamim Hossain M., Sabina Easmin M., Saidul Islam M., Aslam Hossain M., Mamunur R., New Coordination Complexes of Chromium as Cytotoxic and Antimicrobial Agents, Pakistan Journal of Biological Sciences, 4(5), 330-337 (2008) @No $ @ @ Rohrbach K.G. and Schenck S., Control of pineapple heart rot caused by phytophthora with metalaxyl, fosetyl-Al and phosphorus acid, Plant Dis, 69, 320–323 (1985) @No $ @ @ Siegel M.R., Pesticide Selectivity  (J. C. street, ed) Marcel Dekker Inc. New York, 2146, (1975) @No $ @ @ Fehrmann H., Phytopathologische Zeitschrift, 86, 67 (1976) @No $ @ @ Kaars Sijpesteijn A., Systemic Fungicides 2nd ed (R.W. Marsh, ed ) Longman, London, Ch 7 (1977) @No $ @ @ Nathmala and Yadav Rakesh, Bull. chem. soc, Jpn, 70(6), 1331-1337 (1997) @No $ @ @ Fisher D.J. and Hayes, Pesticide Sci, 13, 330-339, (1982) @No $ @ @ Hegde A.N. and Hegde R.K., Plant Pathology Nwsl., 5(1-2), 26 (1987) @No $ @ @ Kim – Beomseok  et al,  J. pest –Management –Science, 56, 12. 1029-1035 (2000) @No $ @ @ Kim - Beomseok. et al, Journal of Agricultural and Food chemistry, 4 8, 5. 1875-1881 (2000) @No $ @ @ Kim – Keunki  et al, J. of antibiotics ,53, 2  131-136 (2000) @No $ @ @ Braicu Cornelia, Andeea Stanila, Mihaela-A.Rotar, Mariana Petran, Carmen Socaciu, Bulletin USAMV-CN, 62 /  (207-212), (2006) @No $ @ @ Ken Yokawa, Tomoko Kagenishi, Kaishi Goto, Tomonori Kawano,  Int J Biol Sci, 5(1), 53-63 (2009) @No $ @ @ Nair, P.K.U. and S. Sasikumaran,Effect of some fungicides on quick wilt disease of black pepper, India cocoa, Areca nut and spices, J., 14(3), 95–96 (1991) @No $ @ @ Young, D.H. and R.A. Slawecki, J. Pesticide Biochemistry and physiology, 69-2, 100-111, (2001) @No $ @ @ Benjaphorn Prapagdee, Chutima Kuekulvong,, Skorn Mongkolsuk, Int J Biol Sci., 5(1), 53-63 (2009) @No $ @ @ Indiradevi, et al ,  Asian. j. chem., 16(1),  493–500 (2004) @No $ @ @ Shamim Hossain M., Kudrat-e-zahan M. , Anwar-ul-Islam S., Sarkar Z., In vitro Antimicrobial and in vivo Cytotoxic Activity of Three Coordination Complexes Synthesized by Mixed Ligands, Pakistan Journal of Biological Sciences,7(7), 1113-1116 (2004) @No $ @ @ Girgaonkar M.V. and Shirodkar S.G, Research Journal of Recent Sciences, 1(ISC-2011), 110-116 (2012) @No $ @ @ More S.M., Shinde V.A., Khan Saiqua, Girde A.V. and Pawar V.N.,Research Journal of Recent Sciences, 1(11), 22-26 (2012) @No $ @ @ Patel Ketan B., Patel Yogesh M. & Patel Raksha B.,Research Journal of Recent Sciences, 2(ISC-2012), 55-60 (2013) @No $ @ @ Javed Ahmad, Iffat khan, Ashfaq Ahmad and kaushar Imam,Research Journal of Recent Sciences, 2(ISC-2012), 41-46 (2013) @No $ @ @ Shelke M.E.,Research Journal of Recent Sciences, 2(ISC-2012) @No $ , 13-14 (2013) @No 
<#LINE#>PCR Detection of Banana Bunchy Top Virus (BBTV) at Tissue Culture Level for the Production of Virus-free Planting Materials<#LINE#>Mahadev@ShelakeRahul,SenthilKumar@Thamilarasan,Kathithachalam@Angappan<#LINE#>22-26<#LINE#>4.ISCA-IRJBS-2013-055.pdf<#LINE#>Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, 641-003, INDIA @ Molecular Cell Physiology Laboratory, Department of Applied Bioresources, Faculty of Agriculture, 3-5-7 Tarumi, Ehime University, Matsuyama, 790-8566, JAPAN @ Department of Horticulture, Sunchon National University, 413 Jungangno, Suncheon, Jeonnam 540-742, REPUBLIC OF KOREA<#LINE#>9/3/2013<#LINE#>12/4/2013<#LINE#>Banana bunchy top disease, caused by Banana bunchy top virus (BBTV), is a serious disease that affects the productivity of banana to a greater extent. The production of virus-free planting material is an efficient method in controlling this disease and increase productivity. In vitro propagation, especially meristem tip culture, has played a key role for obtaining a large number of virus free, homogenous planting materials in plantains and bananas (Musa spp.). In our previous study we have demonstrated that direct regeneration from male floral meristems is a rapid and simple method for clonal and mass propagation of Musa spp. cultivars ‘Virupakshi’ and ‘Sirumalai’ (AAB). Detection of BBTV by polymerase chain reaction (PCR) is very reliable and economically important for testing micropropagated banana plantlets for the absence of virus before release to farmers. In this study we have developed a protocol for early diagnosis of BBTV at tissue culture level itself through optimizing the Polymerase Chain Reaction (PCR) method. We designed the viral gene specific primers [coat protein (CP) gene and nuclear shuttle protein (NSP) gene] and standardize PCR conditions for detection of presence of BBTV. All the regenerated plantlets were tested for BBTV presence with standardized PCR and found to be completely free of virus. PCR tests are more sensitive than ELISA tests and are better to be suited to plant tissue with lower number of pathogens, such as in tissue culture conditions. <#LINE#> @ @ Dale J.L., Banana bunchy top: an economically important tropical plant virus disease, Advances in Virus Research,  33,  325-333 (1987) @No $ @ @ Hafner G.J., Harding R.M. and Dale J.M, Movement and transmission of banana bunchy top virus DNA component one in bananas, Journal of General Virology, 76,  2279-2285, (1995) @No $ @ @ Burns T.M., Harding R.M. and Dale J. L., The genome organization of banana bunchy top virus: analysis of six ssDNA components, Journal of General Virology,  76,  1471-1482 (1995) @No $ @ @ Harding R.M., Burns T.M., Hafner G.J., Dietzgen R. and Dale J. L., Nucleotide sequence of one segment of the banana bunchy top genome contains a putative replicase gene, Journal of General Virology, 74, 323-328, (1993) @No $ @ @ Wanitchakorn R., Harding R.M. and Dale J. L., Banana bunchy top virus DNA-3 encodes the viral coat protein, Archives of Virology,  142, 1673-1680 (1997) @No $ @ @ Wanitchakorn R., Hafner G.J., Harding R.M. and Dale J.L., Functional analysis of proteins encoded by banana bunchy top virus DNA-4–6,  Journal of General Virology,  81,299–306 (2000) @No $ @ @ Thomas J.E., Geering A.D.W., Dahal G., Lockhart B.E.L. and Thottappilly G., Virus and virus-like diseases of major crops in developing countries, in Banana and plaintain.Edited by G. Loebenstein & G. Thottappilly. Dordrecht: Kluwer Academic Publishers, 477–496,  (2003) @No $ @ @ Novak F.J., Afza R., van Duren M., Perea-Dallos M., Conger B.V. and Xiaolang T., Somatic embryogenesis and plant regeneration in suspension cultures of dessert (AA and AAA) and cooking (ABB) banana (Musa spp.), Biotechnology,7, 154–159 (1989) @No $ @ @ Ganapathi T.R., Suprasanna P., Bapat V.A., Kulkarni V.M. and Rao P.S., Somatic embryogenesis and plant regeneration from male flower buds in banana, Current Science,  76,  1228-1231 (1999) @No $ @ @ Kulkarni V. M., Varshney L.R., Bapat V.A. and Rao P.S., Somatic embryogenesis and plant regeneration in a seeded banana [Ensete superbum (Roxb.) Cheesman], Current Science,  83,  939-941 (2002) @No $ @ @ Assani A., Haicour R., Wenzel G., Cote F., Bakry F., Foroughi-Wehr F., Ducreux G.M., Aguillar E.A. and Grapin A., Plant regeneration from protoplasts of dessert banana cv. Grandnaine (Musa spp., Cavendish sub-group AAA) via somatic embryogenesis, Plant Cell Reports,  2,  482-488 (2001) @No $ @ @ Shelake R. M., Angappan K. and Murugan M., An efficient protocol for large-scale plantlet production from male floral meristems of Musa spp. cultivars Virupakshi and Sirumalai, In Vitro Cellular & Developmental Biology – Plant,  47,611-617 (2011) @No $ @ @ Wu R. Y. and Su H. J., Production of Monoclonal Antibodies Against Banana Bunchy Top Virus and Their Use in Enzyme-Linked Immunosorbent Assay, Journal of Phytopathology,  128, 203–208 (1990) @No $ @ @ Dietzgen R.G. and Thomas J.E., Properties of virus-like particles associated with banana bunchy top disease in Hawaii, Indonesia and Tonga, Australasian Plant Pathology,  20, 161-165 (1991) @No $ @ @ Geering A.D.W. and Thomas J.E., A comparison of four serological tests for the detection of banana bunchy top virus in banana, Australian Journal of Agricultural Research,  47, 403–412 (1996) @No $ @ @ Xie W.S. and Hu J.S., Molecular cloning, sequence analysis and detection of banana bunchy top virus in Hawaii, Phytopathology  85, 339–347 (1994) @No $ @ @ Hafner G.J., Harding R.M. and Dale J.M., A DNA primer associated with banana bunchy top virus, Journal of General Virology,  78,  479–486 (1997) @No $ @ @ Chandrasekar A., Kalaiponmani K., Elayabalan S., Kumar K.K., Angappan K. and Balasubramanian P., Screening of banana bunchy top virus through multiplex PCR approach, Archives of Phytopathology and Plant Protection,  44,1920-1925 (2011) @No $ @ @ Harding R.M., Sadik A.S., Bahieldin A. and Dale J.L., A sensitive detection of banana bunchy top nanovirus using molecular genetic approaches. Arabian Journal of Biotechnology,  3,  103-114 (2000) @No $ @ @ Galal A.M., Use of Polymerase Chain Reaction for Detecting Banana Bunchy Top Nanovirus,  6, 53-56, (2007) @No $ @ @ Mansoor S., Qazi J., Amin I., Khatri A., Khan I.A., Raza S., Zafar Y. and Briddon R.W., A PCR-based method, with internal control, for the detection of Banana bunchy top virus in banana, Molecular Biotechnology,30, 167-169 (2005) @No 
<#LINE#>Anti-Oogenic Evaluation of Seed Extract of abrus Precatorius L. in Swiss Albino Mice<#LINE#>Asha@Arora<#LINE#>27-30<#LINE#>5.ISCA-IRJBS-2013-063.pdf<#LINE#>Deptt. of Biotechnology, BNPG College, Udaipur, Rajasthan, INDIA<#LINE#>19/3/2013<#LINE#>10/4/2013<#LINE#>Ethno-botanical reports from Rajasthan reveals utilization of Abrus precatorius L. seeds to check ova formation. So to evaluate its potential ovarian histo-architectural studies were carried out on swiss albino mice treated by body weight dependent seed extract. Ovarian histological observation of test drug treated animals show follicles in different stages of atresia and deviated oestrous cycle revealing its potential as antifertility source. This effect diminishes with time and proceeding restoration of folliculogenesis can be observed after fifteen days of the drug ingestion. Hence the present study embodies Abrus to be prolonged oral fertility regulator. <#LINE#> @ @ Katewa S.S. and Arora A., Some plants in folk medicine of Udaipur district (Rajasthan), Ethnobotany,9, 48–51 (1997) @No $ @ @ Jain A., Katewa S.S., Chaudhary B.L. and Galav P., Folk herbal medicines used in birth control and sexual diseases by tribals of southern Rajasthan, India, Journal of Ethnopharmacology, 90(1), 171-177 (2004) @No $ @ @ Arora R., Gill N.S., Kaur S. and Jain A.D., Phytopharmacological evaluation of ethanolic extract of the seeds of Abrus precatorius (L.), Journal of Pharmacology and Toxicology, 6(6), 580-588 (2011) @No $ @ @ Bagaria A., Surendranath K., Ramagopal U.A., Ramakumar  S. and Karande A.A., Structure-function analysis and insights into the reduced toxicity of Abrus precatorius agglutinin I in relation to abrin, J Biol.Chem., 281, 65-74 (2006) @No $ @ @ Agarwal S.S., Ghatak N., Arora R.B. and Bhardwaj M.M., Antifertility activity of the roots of Abrus precatoriusL. Pharmacological Research Communications, 2(2), 159-163 (1970) @No $ @ @ Bhatt  N.I., Chawla S.L. and  Rao M.V., Contraceptive evaluation of seed extracts of Abrus  precatorius L. in male mice (Mus Musculus),Journal of Herbal Medicine and Toxicology, 1(1),47-50 (2007) @No $ @ @ Zia-Ui-Haque A., Qazi M.H. and Hamdard M.E., Studies on the antifertility properties of active components isolated from the seeds of Abrus precaiorius L. II. Pak J. Zool., 15,141-146 (1983) @No $ @ @ Khan Z.F., Saeed M.A. and Ahmed E., Oxytoxic activity and toxic effects of Globulins of Abrus seeds (Scarlet variety) in Rabbits, J of Islamic Academy of Sciences, 6(2),108-113 (1993) @No $ @ @ Rugh R., The mouse, its reproduction and development, Burgess, Minneapolis(1968) @No $ @ @ Segal S.J. A review of research on physiological control of fertility, J. Reprod. fertil.,2, 533 (1961) @No $ @ @ Wischnitzer S., The maturation of the ovum and the growth of the follicle in the mouse ovary, Anat.Rec.,166, 399 (1966) @No $ @ @ Hadek R., The structure of the mammalian egg, Int.Rev.Cytol.,18, 29 (1965) @No $ @ @ Ingram D.C., The effect of gonadotrophin and estrogens on ovarian atresia in the immature rat, Ist ed., , 247-273 Academic Press New York (1959) @No $ @ @ Desai V.B.  and Sirsi M., The effect of Abrusprecatorius on pregnancy of mice, Curr. Sci,33, 585-587 (1964) @No $ @ @ Okoko II, Osinubi A.A., Olabiyi O.O., Kusemijiu T.O., Noronha C.C., Okanlawon A.O., Anti-ovulatory and Anti-implantation  potential of the methanolic extract of seeds of Abrus precatorius  in the rat, Endocr Pract,11, 554-560 (2010) @No $ @ @ Pokharkar R., Saraswat R., Bhavare V., and Kanawade M. GCMS Studies of Abrus Precatorius, Pharmacologyonline  2, 1178-1189 (2011) @No 
<#LINE#>Isolation and Characterization of Some Indian Hyphochytriomycetes<#LINE#>ManishKumar@Dubey,R.S.@Upadhyay<#LINE#>31-34<#LINE#>6.ISCA-IRJBS-2013-064.pdf<#LINE#>Laboratory of Mycopathology and Microbial Technology, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi- 221 005, Uttar Pradesh, INDIA<#LINE#>19/3/2013<#LINE#>8/4/2013<#LINE#>In the present study, three species of Hyphochytriomycetes were isolated, identified and described. They are Rhizidiomyces hirsutus Karling, Rhizidiomyces apophysatus Zopf and Rhizidiomyces bulbosus Karling. Among them, Rhizidiomyces bulbosus Karling species is being reported for the first time in India.  <#LINE#> @ @ Kirk P.M., Cannon P.F., David J.C. and Stalpers J.A., Ainsworth& Bisby`s Dictionary of the fungi, 9thed- CABI Publishing, Wallingford, Oxon, UK (2001) @No $ @ @ Fuller M.S., Phylum Hyphochytriomycota, In: Handbook of Protoctista, Eds., L. Margulis, J.O. Corliss, M. Melkonian, and D.J. Chapman. Jones and Bartlett, Boston, MA, 380–387 (1990) @No $ @ @ Berbee M.L. and Taylor J.W., Fungal phylogeny, In: Molecular Fungal Biology, Eds., R.P. Oliver and M. Schweizer, Cambridge University Press, 21-77 (1999) @No $ @ @ Karling J.S., The life history of Anisolpidium ectocarpii gen. nov.et sp. nov., and a synopsis and classification of other fungi with anteriorly uniflagellate zoospores, Amer. J. Bot., 30, 637–648 (1943) @No $ @ @ Karling J.S., Some zoosporic fungi of New Zealand. IX. Hyphochytriales or Anisochytridiales, Sydowia, 20, 137–143(1967)@No $ @ @ Alexopoulos C.J., Mims C.W. and Blackwell M., IntroductoryMycology, 4th ed., John Wiley and Sons, Inc. (1996) @No $ @ Fuller M.S., Hyphochytriomycota, In “The Mycota,” Vol. VII, “Systematics and Evolution” (D.J. McLaughlin, E.G. McLaughlin, and P.A. Lemke, eds.), Part A, Springer-Verlag, Berlin, 73–80 (2001)  @No $ @ @ Chaudhuri H. and Kochhar P.L., Indian water moulds. I., Proc. Indian Acad. Sci. Sect., B, 2, 137-154 (1935)  @No $ @ @ Mundkur B.B., Fungi of India, Suppl. I., Sci. Monogr. Counc. Agr. Res., India (1938)  @No $ @ @ Karling J.S., Indian anisochytrids, Sydowia, 17, 193-196 (1964)  @No $ @ @ Sparrow F.K.., Aquatic Phycomycetes, 2nd ed, The University of Michigan Press, Ann Arbor, 743-767 (1960)  @No $ @ @ Fuller M.S. and Jaworski A., Zoosporic fungi in teaching and research, Southeastern Pub. Co., Athens, Georgia, 303 (1987)  @No $ @ @ Karling J.S., Chytridiomycetarum Iconorgraphia, Luberecht and Cramer, Monticello, New York, 383-392 (1977)  @No $ @ @ Karling J.S., Rhizidiomyces hirsutus sp. nov., a hairy anisochytrid from Brazil, Bull. Torrey Bot. Club., 72, 47-51 (1945)  @No $ @ @ Chen S.F., Anisolpidium saprobium and Rhizidiomyces hirsutus, new records of Hyphochytriomycetes (Hyphochytriales) in Taiwan, Fung. Sci., 22, 79–83 (2007)  @No $ @ @ Karling J.S., Zoosporic fungi of Oceania I, J. Mitchell Soc., 84, 166–178 (1968)  @No $ @ @ Zopf W., Zur Kenntnis der Phycomyceten. I. Zur Morphologie und Biologie der Ancylisteen und Chytridiaceen, Nova Acta Acad. Leop.-Carol, 47, 143-236 (1884)  @No $ @ @ Fuller M.S., Growth and development of the water mold Rhizidiomyces in pure culture, Amer. Jour. Bot., 49, 64-71 (1962)  @No $ @ @ Maurizio A., Zur Kenntniss der schweizerischen Wasserpilze nebst Angaben uber eine neue Chytridine, Jahr. Nat. Gesell. Graubundens, 38, 9-38 (1895)  @No $ @ @ Coker W.C., The Saprolegniaceae with notes on other water molds, Univ. North Carolina Press, Chapell Hill, North Carolina(1923)  @No $ @ @ Couch J.N., Observations on cilia of aquatic Phycomycetes, Science, 88, 476 (1938)  @No $ @ @ Couch J.N., Technic for collection, isolation, and culture of chytrids, J. Elisha Mitchell Sci. Soc., 55, 208-214 (1939)  @No $ @ @ Couch J.N., The structure and action of the cilia in some aquatic Phycomycetes, Amer. Jour. Bot., 28, 704-713 (1941)  @No $ @ @ Whiffen A.J., Cellulose decomposition by the saprophytic chytrids, J. Elisha Mitchell Sci. Soc, 57, 321-330 (1941)  @No $ @ @ Sparrow F.K., Observations on the aquatic fungi of Cold Spring Harbor, Mycologia, 24, 268-303 (1932)  @No $ @ @ Tokunaga Y., Studies on the aquatic chytrids of Japan. III. Rhizidiaceae, Trans. Sapporo. Nat. Hist. Soc., 13, 388 (1934)  @No $ @ @ Gaertner A., Über das Vorkommen niederer Erdphycomyceten in Afrika, Schweden, und an einigen mitteleuropäischen Standorten, Arch. f. Mikrobiol., 21, 4-56 (1954) @No
<#LINE#>Habitat Preference of Rotifers Inhabiting some Waters of Jammu province, J&K, India<#LINE#>K.K.@Sharma,Sarbjeet@Kaur,Antal@Neha,Shvetambri<#LINE#>35-38<#LINE#>7.ISCA-IRJBS-2013-067.pdf<#LINE#> Department of Zoology, University of Jammu, Baba sahib Ambedkar road, Jammu, J&K-180006, INDIA<#LINE#>25/3/2013<#LINE#>1/4/2013<#LINE#>The water sources running in this stretch of J&K state are both lentic and lotic including pools, ponds, lakes, streams to mighty rivers. These water bodies varying from seasonal to perennial share a rich amount of aquatic biodiversity among which rotifers is one important group. In an attempt to enlist rotifers 40 lotic and 45 lentic waters were scanned from which 106 rotifer species were recorded. There was seen the presence of both classes (monogononta and digononta) in these waters. Major share of rotifers preferred lentic conditions but 9 species showed an affinity for lotic conditions. <#LINE#> @ @ Kadam S.S. and Tiwari L.R., Zooplankton Composition in Dahanu Creek-West Coast of India, Res. J. Recent Sci.,1(5), 62-65 (2012) @No $ @ @ Battish S.K., Fresh water Zooplankton of India, Oxford and IBH Publishing Co. Pvt. Ltd, New Delhi (1992) @No $ @ @ Mageed A.A.A., Biomass production and turnover rate of zooplankton in Lake Manzala (South Mediterranean sea, Egypt), Egyt. J. Aqua. Res., 32, 158-167 (2007) @No $ @ @ Kumar S., Dutta S.P.S., Malhotra Y.R. and Kumari V., An ecological study of rotifers in Kunjwani pond, Jammu, Hydrobiol., , 41-45 (1991) @No $ @ @ Nayana S.R. and Pejaver M., New records of Rotifers from Thane lakes (Maharashtra), J. Aqua. Biol., 20(1), 9-14 (2005) @No $ @ @ Boon P.I. and Shiel R.J., Grazing on Bacteria by zooplanktons in Australian Billabongs, Aust. J. Mar.Freshwat. Res., 41(2), 247-257 (1990) @No $ @ @ Duraggan C.I., The ecology of periphytic rotifers, Hydrobiologia, 446/447, 139-148 (2001) @No $ @ @ Fontaneto D. and Ricci C., Spatial gradients in species diversity of microscopic animals: The case of Bdelloid rotifers at high altitudes, J. Biogeo., 33, 1305 (2006) @No $ @ @ Rajashwar M., Vijaykumar K and Zeba Paerveen, Seasonal variations of zooplankton community in Fresh water reservoir Gulberga District, Karnataka, South India, International Journal of Systems Biology, 2(1), 6-11 (2010) @No $ @ @ Choudhary S. and Singh D.K., Zooplankton population of Boosra Lake at Muzaffarpur, Bihar, India, Environ. Ecol., 17, 444-448 (1999) @No $ @ @ Arora H.C., Responses of rotifers to variation in some ecological factors,Proc. Ind. Acd. Sci., 63, 57-66 (1966) @No $ @ @ Akhtar R., Assessment of water quality of two high altitude ponds (Sarkoot and Shalimar) with special reference to fish performance. M.Phil Dissertation, University of Jammu, Jammu (2003) @No $ @ @ Yousuf A.R. and Quadri M.Y., Seasonal fluctuations of zooplankton in Lake Manasbal, Kashmir,Ind. J. Ecol. 12,354-359 (1985) @No $ @ @ Thomas S. and Azis A, Plankton abundance and zonal differentiation in Peppara reservoir, Kerala, Ind. J. Environ Ecoplan., 1, 22-25 (1998) @No $ @ @ Koste W., Rotatoria Die Rädertiere Mitteleuropas bergründet von Max Voigt-Monogononta. 2. Auflage neubearbeitet von Walter Koste. Berlin, Gebrüder Borntraeger, 673 (1978) @No $ @ @ Tripathi A.K. and Chishty N., Rotifer Diversity in a Semiurban Shallow Perennial Water Body: A Case Study of Jalaser Tank Mandalgarh, India, I. Res. J. Environmen. Sci.,1(1), 42-45 (2012) @No $ @ @ Hashemzadeh F. and Venkataramana G. V., Impact of Physico-Chemical Parameters of Water on Zooplankton Diversity in Nanjangud Industrial Area, India, I. Res. J. Environmen Sci.,1(4), 37-42 (2012) @No $ @ @ Balvay G. and Laurent M., Long term quantitative evolution of rotifers during the eutrophication in the Lake Geneva, Aquat. Sci., 52(2), 162-175 (1990b) @No $ @ @ Pennak W.R., Freshwater invertebrates of United States, 2rdEdn. A wiley intersc pub., John Wiley and Sons, New York, 803 (1989) @No $ @ @ Jan N.,Ecology of Janipur Pond (Jammu) with special reference to zooplankton dynamics. M.Phil Dissertation, University of Jammu, Jammu (2005) @No $ @ @ De Smet W.H., Notes on the monogonant rotifers from submerged mosses collected on Hopen (Svalbarb), Fauna Norv. Sec. A., 11, 1-8 (1990) @No $ @ @ Pandit K.A. and Yousuf A.R., Rotifers community in some Kashmir Himalayan lakes of varied tropic status,Journal of Research and Development, 3 (2003) @No $ @ @ Schmid-Araya J.M., Distribution and population dynamics of rotifers in bed sediments, Hydrobiologia, 313-314, 279-290 (1995a) @No