International Science Community Association International Research Journal of Biological Sciences 14 4 2025 11 10 Utilization of Grape Seed oil, Linalool and Drakshsava for Improvement of Quality Silk-Cocoons from Silk Spinning Worm, Bombyx mori (L) 1 10 EN Khyade Vitthalrao Bhimasha 1 Department of Zoology, Sharadabai Pawar Mahila Arts, Commerce and Science College, Shardanagar Tal. Baramati Dist. Pune – 413115, India Khanapure Nikhil Pandit 2 Department of Chemistry, Punyashlok Ahilyadevi Holkar Solapur University, Solapur, Maharashtra-413 255, India > Research Paper 2025 4 10 2025 11 10 The nutrition quality and health of larval instars exert influence on quality of the silk yield in sericulture. The acetone solutions of oil from Grape-Seeds (10 ml oil from Grape-Seeds dissolved in 90 ml acetone) and ten microliters of acetone solutions of 100 ppm linalool (one part of a substance per one million parts of a solution / mg per liter) were utilized in this attempt through the topical applications to the fifth stages of larvae of silk spinning worm, Bombyx mori (L) (Race: Double Hybrid). The larvae fed with leaves of mulberry treated with water solution of Drakshasav; group of larvae topically applied with acetone solutions of oil from Grape-Seeds (10 ppm) followed by feeding leaves of mulberry treated with aqueous solution of Drakshasav were also maintained. Fifth Instar Larval Life Duration (Hours) and Tissue-Somatic-Index (T.S.I.) of silk-glands in fifth instar silk spinning worms of control group, group from treated with oil from Grape-Seeds; Linalool treated group; group fed with leaves of mulberry treated with water solution of Drakshasav and group treated with oil of Grape-Seeds treated (topical) followed feeding with leaves of mulberry treated with water solution of Drakshasav were recorded 145.33 ( 13.786), 31.426; 168.73( 13.221), 52.625; 177.46 ( 13.786), 52.728; 162.87(14.572), 52.759 and 168.58(18.789), 53.854 units respectively. The Cocoon-Shell-Ratio of control group, Grape-Seed-Oil treated group; Linalool treated group; group fed with leaves of mulberry treated with aqueous solution of Drakshasav and group treated with Grape-Seed-Oil treated acetone (topical) followed feeding with leaves of mulberry treated with water solution of Drakshasav were recorded 19.422; 23.970; 27.989; 28.048 and 28.378 units respectively. Denier scale of silk filament spun by fifth instar silkworms of control group, Grape-Seed-Oil treated group; Linalool treated group; group fed with leaves of mulberry treated with water solution of Drakshasav and group treated with Grape-Seed-Oil treated acetone (topical) followed feeding by leaves of mulberry treated with aqueous solution of Drakshasav were recorded 3.243; 4.706; 4.793; 4.882 and 4.948 units respectively. The range of improvements of tissue-somatic-index (TSI) of silk glands; Shell-Ratio of cocoon and Denier-scale of silk-filament through treatment was 52.625 to 53.854; 23.970 to 28.378 and 4.706 to 4.948 respectively. Efficient use of source of juvenoids like linalool, grape seed oil and drakshasav in desired solvent for treating the larvae serves to orchestrate the progress of metamorphosis. Copyright@ International Science Community Association