International Science Community Association International Research Journal of Biological Sciences 14 3 2025 08 10 Review: Isozyme Variation in Indian Muscid flies 38 43 EN Mali Pinky J 1 Biology Department, B.K.M. Science College, Valsad, Gujarat, India and Biosciences Department, Veer Narmad South Gujarat University, Surat, Gujarat, India Patel Kalpesh 2 Biology Department, B.K.M. Science College, Valsad, Gujarat, India Nichat Avinash R. 1 Department of Zoology, M.M.V. P.G. College Bhakhara, Dhamtari, Chhattisgarh, India Nayak Jashavant 2 Department of Zoology, Govt. Dudhadhari Bajrang Girls Postgraduate College Raipur, Chhattisgarh, India Sori Premlal 3 Department of Botany, Govt. Mahaprabhu Vallabhacharya Postgraduate College Mahasamund, Chhattisgarh, India Warte Hitesh Kumar 4 Department of Zoology, Govt. Pt. Shyam Shankar Mishra College Deobhog, Gariyaband, Chhattisgarh, India Khiham J. 1 Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India Nanda P. 2 Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India Longkho K. 3 Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India Bo K. 4 Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India Chaudhariq Bhesh Raj 1 Department of Zoology, Mahendra Multiple Campus, Nepalgunj, Nepal Institute of Science and Technology, Tribhuvan University, Nepal Srivastava Deepa 1 Plant Pathology Laboratory, Department of Botany, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, India Tripathi Manisha 1 Department of Zoology, S.P.M. Govt. Degree College, University of Allahabad, Prayagraj -211013, India Agrawal Uma Rani 2 Department of Zoology, C.M.P. Degree College, University of Allahabad, Prayagraj, 211002, India Tripathi Jaya 3 Department of Zoology, Iswar Saran Degree College, University of Allahabad, Prayagraj - 211004, India > Review Paper 2025 6 19 2025 08 10 Isozymes or isoenzymes are multiple molecular forms of an enzyme that perform the same catalytic function but differ in their biochemical, structural and electrophoretic properties. These variants of isoenzymes are of great importance to population genetics studies and evolutionary studies. Isozyme variation has been studied in a large and diverse group of flies of the family Muscidae. Isozyme studies have been used to explore polymorphism, seasonal, temporal and spatial genetic variations. This review emphasizes the relevance of isozyme analysis in understanding genetic, spatial and environmental diversity in Muscid flies offering a road map for future research. Copyright@ International Science Community Association