International Science Community Association International Research Journal of Biological Sciences 14 2 2025 05 10 Genomic characterization of multidrug-resistant Vibrio Cholerae O1 El Tor strains across diverse Indian geographic regions 37 45 EN D. Doshita 1 Department of PG studies in Biotechnology, Nrupathunga University, NT road, Bangalore, India Khanum Saniya 2 Department of PG studies in Biotechnology, Nrupathunga University, NT road, Bangalore, India H. Anusha 3 Department of PG studies in Biotechnology, Nrupathunga University, NT road, Bangalore, India Khabade Sarina P. 4 Department of PG studies in Biotechnology, Nrupathunga University, NT road, Bangalore, India A. Geeta Rautela 1 Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India B. Rose Rizvi 2 Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India Samantaray Debajani 1 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha, India Mishra Atmaja Elina 2 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha, India Panda Subhransu 3 Siksha ‘O’ Anusandhan University, Bhubaneswar, Odisha, India Mohapatra Himadri Das 4 Siksha ‘O’ Anusandhan University, Bhubaneswar, Odisha, India Gupta Nibha 5 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha, India Sardar Shatavisa 1 Post Graduate Department of Zoology, Barasat Govt. College; 10, K.N.C. Road, Barasat, 24 Pgs North, Kolkata-700124, West Bengal, India Paul Sneha 2 Post Graduate Department of Zoology, Barasat Govt. College; 10, K.N.C. Road, Barasat, 24 Pgs North, Kolkata-700124, West Bengal, India Ghosh Jayati 3 Post Graduate Department of Zoology, Barasat Govt. College; 10, K.N.C. Road, Barasat, 24 Pgs North, Kolkata-700124, West Bengal, India Sarkar Sounak 1 MATS School of Sciences, MATS University, Raipur, Chhattisgarh, India Saraf Ashish 2 MATS School of Sciences, MATS University, Raipur, Chhattisgarh, India Dutta Joystu 3 Department of Environmental Science, Sant Gahira Guru Vishwavidyalaya Sarguja, Ambikapur, Chhattisgarh, India > Research Paper 2025 2 28 2025 05 10 Cholera poses a major health risk in several countries worldwide, even in the present century. Hence, it is very important to develop new therapeutic and diagnostic methods for restricting the transmission of the disease. Continued research in the field is necessary for understanding how the dynamics of bacterial virulence, pathogenesis and survival strategies keep changing. In the present study, a total of 20 clinical Vibrio cholerae O1 four strains from each year, from all over the country, were selected from different parts of India were investigated to analyze their genotypic and phenotypic dynamicity. The strains studied mostly used Ogawa and T-27 as the prevalent phage type. The study revealed an alarming situation of the emergence of Multidrug resistance. These strains from India emerged in the form of Haitian ctxB and Haitian tcpA. This is quiet alarming to note the shifting of genotype in V. cholerae strains in India. PFGE analysis showed that overall PFGE similarity between the strain about 80% with fourteen different patterns. Additionally, rabbit ileal loop assay showed the toxicity of V. cholerae strains. Overall, the study showed the emergence of multidrug resistance V.choleraehybrid strain circulating throughout the country. Copyright@ International Science Community Association