International Science Community Association International Research Journal of Biological Sciences 14 1 2025 02 10 In vitro anticoagulant and antioxidant activities of stem bark extracts of Piliostigma thonningii (Schumach.) Milne-Redh 32 37 EN Baba Hussaina Sani 1 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State Nas Faruk Sani 2 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State Maigari Ahmad Kabir 3 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State Abubakar Salisu 4 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State and Biotechnology Advanced Research Centre, Sheda Science and Technology Complex, P.M.B. 186, Garki, Abuja, Nigeria Wilson Victoria 1 Department of Plant Science and Biotechnology, Rivers State University, Port Harcourt, Rivers State, Nigeria Goodlife Eugenia Ebene 2 Department of Plant Science and Biotechnology, Rivers State University, Port Harcourt, Rivers State, Nigeria Patle Prajakta 1 Amity Institute of Biotechnology, Amity University Chhattisgarh, Raipur-493225, India Meshram Anju 2 Amity Institute of Biotechnology, Amity University Chhattisgarh, Raipur-493225, India Mishra Atmaja Elina 1 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha-751015, India Gupta Nibha 2 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha-751015, India Argawanon Jessica A. 1 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Advincula Jenelle 2 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Nolasco Glen S. 3 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Dicuangco John Dave A. 4 Don Honorio Ventura State University, Bacolor, Pampanga, Philippines Arcilla Marilyn S. 5 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Marcelin Kouassi Konan Armand 1 Laboratory of Biology and Health, Unit of –Biochemical-Pharmacodynamics, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, PO BOX 582 Abidjan 22, Côte d'Ivoire Julien Golly Koffi 2 Pasteur Institute of Côte d'Ivoire, Immunity Biology Pole, PO BOX 490 Abidjan 01, Côte d'Ivoire Bessel N’guessan Elouaflin Ettienne De 3 Laboratory of Biology and Health, Unit of –Biochemical-Pharmacodynamics, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, PO BOX 582 Abidjan 22, Côte d'Ivoire David N’guessan Jean 4 Laboratory of Biology and Health, Unit of –Biochemical-Pharmacodynamics, Félix Houphouët-Boigny University of Abidjan, Côte d'Ivoire, PO BOX 582 Abidjan 22, Côte d'Ivoire > Research Paper 2024 10 30 2025 02 10 Thrombotic diseases, i.e. diseases related to blood clotting, are currently a major health problem and one of the main causes of mortality in the world. In this study, the anticoagulant and antioxidant activities of stem bark extracts of Piliostigma thonningii were evaluated. The anticoagulant activity was evaluated on citrated and depleted plasma using two chronometric tests: prothrombin time (PT) and activated partial thromboplastin time (aPTT). The phytochemical screening of extracts was carried out using specific reagents and the antioxidant activity was determined by the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) assay. The results indicate that the aqueous stem bark extract of Piliostigma thonningii at 80 and 100mg/mL showed significant anticoagulant activity in aPTT test, while no significant effect was observed in PT test. Concerning the hydroethanolic one, the clotting time was significantly prolonged in PT and aPTT tests at concentrations of 80 and 100 mg/mL. The phytochemical analysis revealed the presence of polyphenols, flavonoids, tannins, quinones and alkaloids in aqueous and hydroethanolicstem bark extracts of Piliostigma thonningii. Moreover, both extracts exhibited DPPH scavenging activity with antiradical power (AP) of 5.32 ± 0.03µmol/mg for aqueous (IC50 = 18.8 ± 0.12µg/mL) and 8.6 ± 0.33 µmol/mg for hydroethanolic extract (IC50 = 11.67 ± 0.44µg/mL). Copyright@ International Science Community Association