International Science Community Association International Research Journal of Biological Sciences 14 1 2025 02 10 Histopathological effects of Muntingia Calabura leaf extract against Pomacea Canaliculata using Rice field Mimicking method 26 31 EN Baba Hussaina Sani 1 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State Nas Faruk Sani 2 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State Maigari Ahmad Kabir 3 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State Abubakar Salisu 4 Department of Biological Science, College of Natural and Pharmaceutical Sciences, Bayero University, P.M.B. 3011, Kano, Kano State and Biotechnology Advanced Research Centre, Sheda Science and Technology Complex, P.M.B. 186, Garki, Abuja, Nigeria Wilson Victoria 1 Department of Plant Science and Biotechnology, Rivers State University, Port Harcourt, Rivers State, Nigeria Goodlife Eugenia Ebene 2 Department of Plant Science and Biotechnology, Rivers State University, Port Harcourt, Rivers State, Nigeria Patle Prajakta 1 Amity Institute of Biotechnology, Amity University Chhattisgarh, Raipur-493225, India Meshram Anju 2 Amity Institute of Biotechnology, Amity University Chhattisgarh, Raipur-493225, India Mishra Atmaja Elina 1 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha-751015, India Gupta Nibha 2 Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar, Odisha-751015, India Argawanon Jessica A. 1 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Advincula Jenelle 2 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Nolasco Glen S. 3 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines Dicuangco John Dave A. 4 Don Honorio Ventura State University, Bacolor, Pampanga, Philippines Arcilla Marilyn S. 5 Institute of Arts and Sciences, Mabalacat City College, Mabalacat City, Pampanga, Philippines > Research Paper 2024 10 12 2025 02 10 Pomacea canaliculata, also referred as the golden apple snail, is an invasive species significantly affecting agricultural crop production. Its rapid proliferation, secretion of contaminants like eggs and slime, and aggressive feeding habits have driven the search for safe, toxic-free alternatives to conventional pesticides. Among various plants, Muntingia calabura L., has gained attention for its potential as a natural pesticide. This study aimed to assess the molluscicidal activity of ethanolic leaf extract of M. calabura (ELEMC). Snail biossay was performed in simulated rice field, exposing the P. canaliculata to varying concentrations ranging from 200 to 1000 mg/L for 48 h. Mortality rates were recorded every 12h. Results showed that concentrations from 800 mg/L (T4) to 1000 mg/L (T5) were comparable to the commercial positive control. Probit analysis revealed that the LC50 was at 870.96 mg/L. A very high positive correlation (r=0.8358) was also observed between the concentration and mortality. Histopathological analysis of gills of ELEMC-treated snails revealed severe damage to the gills showing vacuolization, complete loss of cilia, degenerated columnar cells, reduced hemocyte quantity, splitting and degeneration of gill filaments. The molluscicidal activity of the extract may be accounted for from the presence of phytochemicals as revealed from literature. In conclusion, the ELEMC could be a potential source of molluscicide against P. analiculata. Copyright@ International Science Community Association