International Science Community Association International Research Journal of Biological Sciences 2278-3202 12 1 2023 05 10 Urease Inhibitory activity of fractions from Harungana madagascariensis fruit Lam. Ex Poir.(Hyperiaceae) 16 20 EN Sendaydiego Judy P. 1 Biology Department, Xavier University, Corrales Avenue, Cagayan de Oro City, Philippines Sinco Astrid L. 2 Biology Department, Xavier University, Corrales Avenue, Cagayan de Oro City, Philippines Kahlil R. Abejuela Kahlil R. 3 Biology Department, Xavier University, Corrales Avenue, Cagayan de Oro City, Philippines Hinacay Ric Louis 4 Biology Department, Xavier University, Corrales Avenue, Cagayan de Oro City, Philippines Jumawid Nel Kina 5 Biology Department, Xavier University, Corrales Avenue, Cagayan de Oro City, Philippines A.H. Gijo 1 Department of Biological Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria K.J Alagoa 2 Department of Biological Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria Okonkwo Onyinye Blessing 1 Department of Pharmacognosy and Traditional Medicine, Faculty of Pharmaceutical Sciences, Chukwuemeka Odumegwu Ojukwu Unuversity, Igbariam Campus and Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria Afieroho Ozadheoghene Eriarie 2 Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria and Nuclei for Phytomedicines and Chemical Ecology, Central Research Laboratory for Phytomedicines, Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria Abo Kio Anthony 3 Department of Pharmacognosy and Phytotherapy, Faculty of Pharmaceutical Sciences, University of Port Harcourt. Port Harcourt, Nigeria > Research Paper 2022 10 13 2023 05 10 Harungana madagascariensis is used in ethnomedicine to treat stomach diseases, bacterial infections some of which are due to ureolytic pathogens, and anemia among others. This study is a urease inhibition activity guided fractionation of the crude ethanol extract of the fruits of Harungana madagascariensis. The fruit of H. madagascariensis was extracted with 70% aqueous ethanol by cold maceration. The crude aqueous ethanol extract (CEE) was defatted with n-hexane and further partitioned with ethyl acetate to give n-hexane portion (HSF), ethyl acetate portion (ESF) and aqueous portion (ASF). Fractionation of most active ESF was done using chromatographic techniques and it yielded four fractions EA1 –EA4. The modified Berthelot’s colorimetric method was used for the in vitro urease inhibition assay. Standard phytochemical methods were used for phytochemical screening. The trend of percentage (%) inhibition for the crude extract and its partitioned fractions was: CEE(35.03±0.05)>ESF(33.29±0.00)>ASF(17.10±0.06)>HSF(12.97±0.06) at 2.00 mg/ml while the trend of activity for the chromatography fractions from the most active ESF at 2.00 mg/ml was observed thus; EA3(60.74±0.00) > EA4 (26.69± 0.06)>EA2(16.79±0.06)>EA1(14.16±0.06). The most active chromatography fraction EA3 had an IC50 of 1.0 mg/ml. phytochemical investigation of EA3 revealed the presence of anthraquinones and phenolics. This supports the use in ethnomedicine practice, of H. madagascariensis to treat various stomach and urinary diseases and also a potential lead source of urease inhibitors for sustainable agronomy. Copyright@ International Science Community Association