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Isolation and characterization of plant growth promoting non-rhizobial Root nodule bacteria of major legumes in Malawi

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Author Affiliations

  • 1Ministry of Agriculture, Chitedze Agricultural Research Station box 158, Lilongwe, Malawi
  • 2Malawi University of Science and Technology, P.O BOX 5196, Limbe, Malawi
  • 3Ministry of Agriculture, Chitedze Agricultural Research Station box 158, Lilongwe, Malawi
  • 4Department of Forest, Kasungu District Office, P.O BOX 314 Kasungu, Malawi

Res. J. Recent Sci., Volume 7, Issue (8), Pages 24-29, August,2 (2018)

Abstract

Plant Growth Promoting Rhizobacteria (PGPR) are important microorganisms inoculated into agricultural land and act positively to crop production to achieve sustainable agriculture. Rhizobium inoculation is the most commonly used PGPR for production of legumes. However, isolation, reproducibility, competitive survivability and efficacy of rhizobial inoculation need understanding of their compatibility in natural state. To address the problem research has shown that inoculation of nonrhizobial nodule-associated bacteria acts positively on plant growth and nodulation when coinoculated with rhizobia. This study investigated nonrhizobial nodule-associated bacteria for legume crops grown in Malawi. Microbes were isolated from root nodules using yeast extract mannitol agar supplemented with congo red. Biochemical test and genetic characterization using 16S rDNA gene were used for strain identification which was supplemented by testing for the presence of Plant Growth Promoting Traits (PGPT). Results showed diversity of gram-negative nonrhizobial nodule-associated bacteria in the genus of Klebsiella, Leclercia, Enterobacter, Pseudomonas and Enterococcus. Isolated microbes were not host specific and have PGPT. The study puts assumption that these nonrhizobial nodule-associated bacteria isolates are not crop specific but site specific and are responsible for increase in yield, yield components and nitrogen fixation in legume production.

References

  1. Castro-Sowinski S., Herschkovitz Y., Okon Y. and Jurkevitch E. (2007)., Effects of inoculation with plant growth-promoting rhizobacteria on resident rhizosphere microorganisms., FEMS Microbiol Lett., 276(1), 1-11. doi:10.1111/ j.1574-6968.2007.00878.x.
  2. Rajendran G., Patel M.H. and Joshi S.J. (2012)., Isolation and characterization of nodule-associated Exiguobacterium sp. from the root nodules of fenugreek (Trigonella foenum-graecum) and their possible role in plant growth promotion., International journal of microbiology. doi:10.1155/2012/693982.
  3. Ghevariya K.K. and Desai P.B. (2014)., Rhizobacteria of sugarcane: in vitro screening for their plant growth promoting potentials., Res. J. Recent Sci, 3, 52-58.
  4. Leite J., Fischer D., Rouws L.F., Fernandes-Júnior P.I., Hofmann A., Kublik S. and Radl V. (2017)., Cowpea nodules harbor non-rhizobial bacterial communities that are shaped by soil type rather than plant genotype., Frontiers in plant science, 7, 2064, 1-11. doi:10.3389/ fpls.2016.02064.
  5. Sciences P. and Technology C. (2017)., Co-inoculation of phosphate solubilizing bacteria and rhizobia for improving growth and yield of mungbean., Vigna ... 2011;(July 2017).
  6. Argaw A. (2011)., Evaluation of co-inoculation of Bradyrhizobium japonicum and Phosphate solubilizing Pseudomonas spp. effect on soybean (Glycine max L. Merr.) in Assossa Area., Journal of Agricultural Science and Technology, 14(1), 213-224.
  7. Aditya B., Ghosh A. and Chattopadhyay D. (2009)., Co-inoculation effects of nitrogen fixing and phosphate solublising microorganisms on teak (Tectona grandis) and indian redwood (Chukrasiatu bularis)., J. Biol. Sci, 1, 23-32.
  8. Aamir M., Aslam A., Khan M.Y. and Usman M. (2013)., Co-inoculation with rhizobium and plant growth promoting rhizobacteria (PGPR) for inducing salinity tolerance in mung bean under field condition of semi-arid climate., Asian J. Agri. Biol. 1(1), 7-12.
  9. Bourque F.G. (2011)., The Isolation, Identification and Characterization of Endophytes of Sw itchgrass (Panicu m virgatum L .)., Bioenergy Crop, 2011.
  10. Santi C., Bogusz D. and Franche C. (2013)., Biological nitrogen fixation in non-legume plants., Annals of botany, 111(5), 743-767. doi:10.1093/aob/mct048.
  11. Stajković O., De Meyer S., Miličić B. and Willems A. (2009)., Isolation and characterization of endophytic non-rhizobial bacteria from root nodules of alfalfa (Medicago sativa L.)., Botanica serbica, 33(1), 107-114.
  12. Habte M. (1985)., Selective medium for recovering specific populations of rhizobia introduced into tropical soils., Applied and environmental microbiology, 50(6), 1553-1555.
  13. Wedage W.M.M. and Gunawardana D. (2016)., Rhizobial and non-Rhizobial nodulators of Pueraria phaseoloides., 4-8.
  14. Mwafulirwa S., Obiero G., Mpeketula P. and Aboge G. (2017)., Isolation of indigenous glyphosate degrading microbes from selected agro ecological zones of Malawi., 6(12), 1-9.
  15. Zhao L., Xu Y., Sun R., Deng Z., Yang W. and Wei G. (2011)., Identification and characterization of the endophytic plant growth prompter Bacillus cereus strain MQ23 isolated from Sophora alopecuroides root nodules., Brazilian Journal of Microbiology, 42(2), 567-575.
  16. Qureshi M.A., Iqbal A., Akhtar N., Shakir M.A. and Khan A. (2012)., Co-inoculation of phosphate solubilizing bacteria and rhizobia in the presence of L-tryptophan for the promotion of mash bean (Vigna mungo L.)., Soil & Environment, 31(1), 47-54.
  17. Hossain M.Z. and Lundquist P.O. (2016)., Nodule Inhabiting Non-rhizobial Bacteria and Their influence on growth of selected leguminous plants of Bangladesh., Biores Commun, 2, 139-145.
  18. Sharma V., Archana G. and Kumar G.N. (2011)., Plasmid load adversely affects growth and gluconic acid secretion ability of mineral phosphate-solubilizing rhizospheric bacterium Enterobacter asburiae PSI3 under P limited conditions., Microbiological research, 166(1), 36-46. doi:10.1016/j.micres. 2010.01.008.
  19. Kumar G.K., Ram M.R. (2017)., Plant Growth Promoting Characteristics of Non- Rhizobial Strains Isolated From Root Nodules of Vigna Trilobata Cultivars., 7(2), 273-278.
  20. Stajković-Srbinović O.L.I.V.E.R.A., Delić D.U.Š.I.C.A., Kuzmanović D.J.O.R.D.J.E., Protić N.A.D.A., Rasulić N. A.T.A.Š.A. and Knežević-Vukčević J.E.L.E.N.A. (2014)., Growth and nutrient uptake in oat and barley plants as affected by rhizobacteria., Rom. Biotechnol. Lett., 19(3), 9429-9436.
  21. Pandya M., Kumar Naresh G. and Rajkumar S. (2013)., Invasion of rhizobial infection thread by non-rhizobia for colonization of Vigna radiata root nodules., FEMS microbiology letters, 348(1), 58-65. doi:10.1111/1574-6968.12245.
  22. Rugheim A.M.E. and Abdelgani M.E. (2012)., Effects of microbial and chemical fertilization on yield and seed quality of faba bean (Vicia faba)., International information system for the agricultural science and technology, 19(2), 417-422.
  23. Abd-alla M.H., El-enany A.E., Nafady N.A., Khalaf D.M. and Morsy F.M. (2014)., Synergistic interaction of Rhizobium leguminosarum bv. viciae and arbuscular mycorrhizal fungi as a plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soil., Microbiol Res., 169(1), 49-58. doi:10.1016/j.micres.2013.07.007.
  24. Li J.F., Zhang S.Q., Huo P.H., Shi S.L. and Miao Y.Y. (2013)., Effect of phosphate solubilizing rhizobium and nitrogen fixing bacteria on growth of alfalfa seedlings under P and N deficient conditions., Pak. J. Bot, 45(5), 1557-1562.
  25. Sarathambal C. and Ilamurugu K. (2014)., Phosphate solubilising diazotrophic bacteria associated with rhizosphere of weedy grasses., Indian Journal of Weed Science, 46(4), 364-369.
  26. Sachdev D.P., Chaudhari H.G., Kasture V.M., Dhavale D.D. and Chopade B.A. (2009)., Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth., CSIR, 47, 993-1000.
  27. López-Ortega M.D.P., Criollo-Campos P.J., Gómez-Vargas R.M., Camelo-Rusinque M., Estrada-Bonilla G., Garrido-Rubiano M.F. and Bonilla-Buitrago R. (2013)., Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants., Colombian Journal of Biotechnology, 15(2), 115-123.