Change in bacterial community and pollution load in lab-scale bioreactors designed with waste material for the treatment of paper and pulp mill effluent
- 1Institute of Environmental Studies, Kurukshetra University, Kurukshetra-136119, India
- 2Institute of Environmental Studies, Kurukshetra University, Kurukshetra-136119, India
- 3Institute of Environmental Studies, Kurukshetra University, Kurukshetra-136119, India
Int. Res. J. Environment Sci., Volume 7, Issue (5), Pages 19-28, May,22 (2018)
Two lab-scale airlift bioreactors using different waste material such as coconut shells (ALBRc) and gravel (ALBRg) as substrates for bacterial growth and a rotating biological contactor (RBC), were designed for the treatment of paper mill effluents. Change in community during the treatment was assessed and it was found that biodiversity of the bacterial species changed during the acclimatization process, with the replacement of some species with the dominant ones at the end of acclimatization. The results showed Bacillus sp., Flavobacterium sp. Pseudomonas sp. and Arthrobacter sp. as the most dominant species. Maximum bacterial growth was observed in ALBRc (120×106 CFU/ml), followed by RBC (98×106 CFU/ml). Hence, ALBRc was the most effective bioreactor for the microbial biomass production and also for wastewater treatment of paper mill effluents, maximum reduction in pollution load (pH, EC, TDS, BOD, COD and phosphates) was observed in case of ALBRc, (7.05%, 32.9%, 33.9%, 41.8%, 53.7% and 37.4%, respectively). Therefore, it can be concluded that the bacteria related to nutrient removal dominate in the bioreactors and at the same time make the system efficient not only for the wastewater treatment but for biomass production as well.
- Yadav R.D., Chaudhry S. and Dhiman S.S. (2010)., Biopulping and its potential to reduce effluent loads from bleaching of hardwood kraft pulp., Bioresources., 5(1), 159-171.
- Ramos D.L.S.W., Poznyak T., Chairez I., and Córdova R.I. (2009)., Remediation of lignin and its derivatives from pulp and paper industry wastewater by the combination of chemical precipitation and ozonation., J. Hazard. Mater., 169(1-3), 428-434.
- Erickson L.E. (2000)., Bioreactor, In: The Desk Encyclopedia of microbiology, 2nd ed. (Schaechter M., and Lederberg J., eds.)., Academic Press. San Diego, CA., USA., 189-195. ISBN: 0-12-621361-5
- Duque A.F., Bessa V.S. and Castro P.M. (2014)., Bacterial community dynamics in a rotating biological contactor treating 2-fluorophenol-containing wastewater., J. Ind. Microbiol. Biotechnol., 41(1), 97-104.
- Metcalf E. (2003)., Wastewater Engineering: Treatment and reuse., 4th ed., McGraw Hill, New York. ISBN-13: 978-0070418783
- Syron E. and Casey E. (2008)., Membrane-aerated biofilms for high rate biotreatment: Performance appraisal, engineering principles, scale-up, and development Requirements., Environ. Sci. Technol., 42(6), 1833-1844.
- Xia S.Q., Duan L., Song Y., Li J., Piceno Y.M., Andersen G.L., Alvarez-Cohen L., Moreno-Andrade I., Huang C.L., and Hermanowicz S.W. (2010)., Bacterial community structure in geographically distributed biological wastewater treatment reactors., Environ. Sci. Technol., 44(19), 7391-7396.
- Bergey D.H., Holt J.G., Krieg N.R. and Sneath P.H.A. (1994)., Bergey, 9th ed., (Breed R.S., Murray E.G.D., and Smith N.R., eds.) Williams and Wilkims, Baltimore.
- Sharifi-Yazdi M.K., Azimi C. and Khalili M.B. (2001)., Isolation and Identification of Bacteria Present in the Activated Sludge Unit, in the Treatment of Industrial Waste Water., Iranian. J. Public. Health., 30(3-4), 91-94.
- Nishimori E., Kita-Tsukamoto K. and Wakabayashi H. (2000)., Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis., Int. J. Syst. Evol. Microbiol., 50(1), 83-89.
- Raj A., Reddy M.M.K. and Chandra R. (2007)., Identification of low molecular weight aromatic compounds by gas chromatography–mass spectrometry GC–MS. from kraft lignin degradation by three Bacillus sp., Int. Biodeterior. Biodegradation., 59(4), 292-296.
- Franzetti L. and Scarpellini M. (2007)., Characterisation of Pseudomonas spp. isolated from foods., Ann. Microbiol., 57(1), 39-47.
- Sipma J., Osuna M.B., Emanuelsson M.A.E. and Castro P.M.L. (2010)., Biotreatment of industrial wastewaters under transient-state conditions: process stability with fluctuations of organic load, substrates, toxicants, and environmental parameters., Crit. Rev. Environ. Sci. Technol., 40(2), 147-197.
- Paliwal R., Uniyal S. and Rai J.P.N. (2015)., Evaluating the potential of immobilized bacterial consortium for black liquor biodegradation., Environ. Sci. Pol. Res., 22(9), 6842-6853.
- Cabrol L., Malhautier L., Poly F., Lepeuple A-S. and Fanlo J-L. (2012)., Bacterial dynamics in steady-state biofilters: beyond functional stability., FEMS Microbiol. Eco., 79(1), 260-271.
- Singhal V., Kumar A. and Rai J.P.N. (2005)., Bioremediation of pulp and paper mill effluent with Phanerochaete chrysosporium., J. Environ. Biol., 26(3), 525-229.
- Esposito E., Canhos V.P. and Durán N. (1991)., Screening of lignin-degrading fungi for removal of color from Kraft mill wastewater with no additional extra carbon-source., Biotechnology letters, 13(8), 571-576.