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Microbial technology for revegetation in overburden dumps of coal mined area of Assam, India - a review

Author Affiliations

  • 1Rain Forest Research Institute, Sotai, Jorhat-785010, Assam, India
  • 2Rain Forest Research Institute, Sotai, Jorhat-785010, Assam, India
  • 3Institute of Advanced Study in Science and Technology, Guwahati, India

Int. Res. J. Environment Sci., Volume 7, Issue (12), Pages 56-62, December,22 (2018)


Activities of open cast coal mining create large area of overburden dump (OBD) in Makum Coalbelt, Assam. These OBDs are highly acidic and low in organic carbon, nutritional status and microbial activity. The natural process of re-establishment of vegetation on the OBDs is very slow. However, research works indicated that application of microbial including arbuscular mycorrhiza (AM) fungi, phosphate solubilizing bacteria (PSB), lime and organic amendments (FYM) can enhance plant growth on OBDs. Application of natural forest topsoil could stimulate plant growth through improvement of microbial activity in the inert OBDs.


  1. Singh J.S. and Jha A.K. (1993)., Restoration of degraded lend: an overview., Restoration of Degraded Land: Concepts and Strategies, Rastogie Publication, Meerut, India, 1-9. ISBN 10: 8171331637
  2. Hazarika P., Talukdar N.C. and Singh Y.P. (2004)., Arbuscular Mycorrhizal Fungi and Soil Amendments in Restoration and Revegetation of Coal mine Overburden Dumps of Margherita Coalbelt, Assam., Ecology, Environment and Conservation, 10(4), 431-442.
  3. Hazarika P., Talukdar N.C. and Singh Y.P. (2010)., Arbuscular mycorrhizal association in naturally invading plant species in overburden dumps and adjacent natural forest sites of Tikak Colliery, Margherita, Assam, India., Eco. Env. and Cons., 16(1), 13-24.
  4. Parkinson D. (1979)., Microbes, Mycorrhizae and mine spoil., Ecology and Coal resource development, Pergaman Press, New York, 634-642. ISBN: 978-81-322-0850-1 (Print) 978-81-322-0851-8 (eBook). DOI: 10.1007/978-81-322-0851-8.
  5. Visser S., Zak J. and Parkinson D. (1979)., Effects of surface mining on soil microbial communities and process., Ecology and Coal Resource development. (Ed. M.K. Wali), Pergamon Press, New York, 2, 643-651. ISBN: 978-81-322-0850-1 (Print) 978-81-322-0851-8 (eBook). DOI: 10.1007/978-81-322-0851-8.
  6. Zak J.C. and Parkinson D. (1983)., Effects of surface amendation of two mine spoils in Alberta, Canada, on vesicular-arbuscular mycorrhizal development of slender wheatgrass: a 4-year study., Canadian Journal of Botany, 61(3), 798-803.
  7. Kumar U. and Jena S.C. (1996)., Trial of integrated biotechnical approach in biological reclamation of coal mine spoil dumps in South-Eastern Coalfields Limited (SECL), Bilaspur (Madhya Pradesh)., Indian Forester, 122(12), 1085-1091.
  8. Dugaya D., Williams A.J., Chandra K.K., Gupta B.N. and Banerjee S.K. (1996)., Mycorrhizal development and plant growth in amended coal mine overburden., Indian Journal of Forestry, 19(3), 222-226.
  9. Pandya S.R., Patil M.R. and Kharat R.B. (1997)., Revegetation of Coalspoil by Flyash and Pulp and Papermill Waste., Journal of Industrial Pollution Control, 13, 151-157.
  10. Janos D.P. (1980)., Mycorrhizae influence tropical succession., Biotropica, 56-64.
  11. Allen M.F. (1989)., Mycorrhizae and rehabilitation of disturbed arid soils: processes and practices., Arid Land Research and Management, 3(2), 229-241.
  12. Bowen G.D. (1973)., Mineral nutrition of mycorrhizas., Ectomycorrhizas. (Eds. G.C. Marks and T.T. Kozlowski), 151-201. ISBN: 9780124728509(Print), 9780323149495 (ebook).
  13. Gaur A.C. and Rana J.P.S. (1990)., Role of mycorrhizae, Phosphate solubulizing bacteria and their interactions on growth and uptake of nutrients by wheat crops., Trends in Mycorrhizal Research. Proceedings of National Conference on Mycorrhizae (Eds. B. L. Jalali and H. Chand). Hariana Univ. Hisser, India, 14th -16th Feb, 105-106.
  14. Potty V.P. and Harikummer V.S. (1995)., Interaction of vesicular arbuscular mycorrhizal fungi and Phosphobacterium in Sweet potato rhizophere., Proceedings of 3rd national conference on Mycorrhiza: Mycorrhizae: Biofertilizer for the future. ( Eds: Alok Adholeya, Sujan Singh). TERI, India.13th -15th March, 240-244.
  15. Norland M.R., Veith D.L. and Dewar S.W. (1992)., Vegetation response to organic soil amendments on coarse taconite tailing., In Proceedings National Meeting of the American Society for Surface Mining and Reclamation.(Duluth, MN), 341-360.
  16. Noyd R.K., Pfleger F.L. and Norland M.R. (1996)., Field responses to added organic matter, arbuscular mycorrhizal fungi, and fertilizer in reclamation of taconite iron ore tailing., Plant and Soil, 179(1), 89-97.
  17. Hazarika P., Talukdar N.C. and Singh Y.P. (2010)., Effect of application of beneficial microorganisms, lime, organic amendments and topsoil on growth of Crotolaria striata D C in coal mine overburden dump spoils., Asian Jr. of Microbiol. Biotech. Env. Sc., 12(3), 495-504.
  18. Hazarika P., Singh Y.P. and Talukdar N.C. (2003)., Effect of coal mining on soil microbial biomass and nutritional status in different aged overburden dumps and natural recovery pattern., J. Trop. For., 19(3-4), 24-34.
  19. Srivastava S.C. (1999)., Effect of coal mining on microbial biomass and nutrient availability in dry tropical forest of Vindhya Hill region., Journal Tropical Forestry., 15, 15-23.
  20. Woodmansee R.G., Reeder J.D. and Berg W.A. (1980)., Nitrogen in drastically disturbed lands., In: C.T. Youngberg (ed) Forest Soils and Land Use., Proceedings, Fifth North American Forest Soils Conference, Colorado State University Press, Fort Collins, CO,. August, 376-392.
  21. Nandeswar D.L., Dugya D., Mishras T.K., Williums A.J. and Banerjee S.K. (1996)., Natural succession of an age series of coal mine spoils in a subtropical region., Adv. Plant. Sci. Res. India, 3, 105-124.
  22. Jha A.K. and Singh J.S. (1991)., Spoil characteristics and vegetation development of an age series of mine spoils in a dry tropical environment., Vegetation, 97, 63-76.
  23. Prasad R. and Awasthi R.P. (1992)., Nutritional status affected coal mine overburden dumps of Dhanpur in Madhya Pradesh., J. Trop.For., 8(11), 109-118.
  24. Hazarika P., Talukdar N.C. and Singh Y.P. (2010)., Performance of Native Arbuscular Mycorrhizal Plant Species and Soil Amendments in Revegatation of Coalmine Overburden., Global Journal of Environmental Research, 4(3), 192-198.
  25. Hazarika P., Talukdar N.C. and Singh Y.P. (2014)., Arbuscular Mycorrhizal Fungi (AMF) in Revegetated Coal Mine Overburden Dumps of Margherita, Assam, India., Life Sciences Leaflets, 51, 40-58.
  26. Wilson H.A. (1965)., The microbiology of strip-mine spoil., West Virginia University Agriculture Experiment Station Bulletin., 506, 44
  27. Lawrey J.D. (1977)., Soil fungal populations and soil respiration in habitats variously influenced by coal strip-mining., Environmental Pollution, 14(3), 195-205.
  28. Banerjee S.K., Das P.K. and Mishra T.K. (2000)., Microbial and Nutritional Characteristics of Coal Mine Overburden Spoils in Relation to Vegetation Development., Journal of Indian Society of Soil Science., 48(1), 63-66.
  29. Banerjee S.K. (2001)., Natural recovery of iron minespoils of Dalli-Rajhara in Madhya Pradesh., Journal of the Indian Society of Soil Science, 49(2), 380-382.
  30. Soedarjo M. and Habte M. (1993)., Vesicular-arbuscular mycorrhizal effectiveness in an acid soil amended with fresh organic matter., Plant and soil, 149(2), 197-203.
  31. Prasad R. and Shukla P.K. (1985)., Reclamation and revegetation of coal mine overburdens in Madhya Pradesh., Journal Tropical Forestry, 1(1), 79-84.
  32. Linsey D.L., Cress W.A. and Aldon E.F. (1977)., The effects of Endomycorrhizae on growth of Rabbitbrush, Fouwing salt brush and corn in coal mine spoil material., USDA For. Service. Res. Note., RA-343.
  33. Khan A.G. (1981)., Growth responses of endomycorrhizal onions in unsterilized coal waste., New Phytologist, 87(2), 363-370.
  34. Gupta B.N., Singh A.K., Bhowmik A.K. and Banerjee S.K. (1994)., Suitability of different tree species for coal mine overburdens., Annals of Forestry, 2(1), 85-87.
  35. Lambert D.H. and Cole H. (1980)., Effects of Mycorrhizae on Establishment and Performance of Forage Species in Mine Spoil 1., Agronomy journal, 72(2), 257-260.
  36. Nurlaeny N., Marschner H. and George E. (1996)., Effects of liming and mycorrhizal colonization on soil phosphate depletion and phosphate uptake by maize (Zea mays L.) and soybean (Glycine max L.) grown in two tropical acid soils., Plant and soil, 181(2), 275-285.
  37. Anjos J.T. and Rowell D.L. (1987)., The effect of lime on phosphorus adsorption and barley growth in three acidic soils., Plant and Soil, 103, 75-82.
  38. Chabukdhara M. and Singh O.P. (2016)., Coal mining in northeast India: an overview of environmental issues and treatment approaches., International Journal of Coal Science and Technology, 3(2), 87-96. DOI 10.1007/s40789-016-0126-1
  39. Friesen D.K., Juo A.S.R. and Miller M.H. (1980)., Liming and Lime-Phosphorus-Zinc Interactions in Two Nigerian Ultisols: I. Interactions in the Soil 1., Soil Science Society of America Journal, 44(6), 1221-1226.
  40. Naidu R., Tillman R.W., Syers J.K. and Kirkman J.H. (1990)., Lime- alluminium- phosphorus interactions and growth of Leucanea leucocephala L plant growth studies., Plant and Soil, 126, 1-8.
  41. Aldon E.F. (1978)., Endomycorrhizae enhance shrub growth and survival on mine spoils., The reclamation of disturbed arid lands, 174-179. ISBN 0-7732-0886-0.
  42. Daft M.J. and Hacskaylo E. (1977)., Growth of endomycorrhizal and nonmycorrhizal red maple seedlings in sand and anthracite spoil., Forest Science, 23(2), 207-216.
  43. Zak J.C. and Parkinson D. (1982)., Initial vesicular-arbuscular mycorrhizal development of slender wheatgrass on two amended mine spoils., Canadian Journal of Botany, 60(11), 2241-2248.
  44. Amaranthus M.P. and Perry D.A. (1989)., Interaction effects of vegetation type and Pacific madrone soil inocula on survival, growth, and mycorrhiza formation of Douglas-fir., Canadian Journal of Forest Research, 19(5), 550-556.
  45. Helm D.J. and Curling D.E. (1993)., Use of soil transfer for reforestation on abandoned mined lands in Alaska. II. Effects of soil transfers from different successional stages on growth and Mycorrhizal formation by Populous balsamifera and Alnus crispa., Mycorrhiza, 3, 107-114.
  46. Bowen G.D. and Theodorou C. (1979)., Interactions between bacteria and ectomycorrhizal fungi., Soil Biology and Biochemistry, 11(2), 119-126.
  47. Azcon R., Barea J.M. and Hayman D.S. (1976)., Utilization of rock phosphate in alkaline soils by plants inoculated with mycorrhizal fungi and phosphate-solubilizing bacteria., Soil Biology and Biochemistry, 8(2), 135-138.
  48. Falkowski P.G., Fenchel T. and Delong E.F. (2008)., The microbial engines that drive Earth, Science, 320(5879), 1034-1039.
  49. Prasad R. and Mahammad G. (1990)., Effectiveness of Nitrogen fixing Trees (NFT) s in improving microbial status of Bauxite and coal mined out areas., Journal of Tropical Forestry, 6(1), 86-94.
  50. Kugler M. (1986)., Mycorrhizae: The beneficial bounding of plants and fungi., IDRC Report., 15(1), 56.
  51. Jones M.D. and Hutchinson T.C. (1988)., Nickel toxicity in mycorrhizal birch seedlings infected with Lactarius reutus or Scleroderma flavidum. I. Effects on growth photosynthesis, respiration and transpiration., New phytol, 108, 451-459.
  52. Prasad R. (1988)., Technology for wasteland development., Associated Publishing Company New Delhi, India, 27-69. ISBN: 9788185211190
  53. Mohammod G. (1988)., Response of Leucanea leucocephala K-8.to inoculation with Rhizobium, Bacillus- megaterium and Glomus fasciculatum, Leucenea., Research report, 9, 51-52.
  54. Rudawaska M. (1983)., The effect of nitrogen and phosphorus on auxin and cytokine production by mycorrhizal fungi., Arboretum Konickie, 28, 219-236.
  55. Prasad R. and Awasthi R.P. (1992)., Nutritional status affected coalmine overburden dumps of Dhanpur in Madhya Pradesh., J. Trop.For., 8(11), 109-118.
  56. Hazarika P. (2002)., Vesicular Arbuscular Mycorrhizal Fungi of Margherita Coal belt, Assam and Their Significance in Revegetation of Coal mine Spoils., 137 (PhD thesis), FRI (D) University, Dehradun, India. (Unpublished)
  57. Sumner M.E., Fey M.V. and Noble A.D. (1991)., Nutrient status and toxicity problems in Acid soils., Soil Acidity Eds. By B.Ulrich, M.E.Sumner, Springlar Varlag Pub., 149-182. ISBN 978-3-642-74442-6.
  58. Meyer J.R. and Linderman R.G. (1986)., Response of Subteranean clover to dual inoculation with vesicular arbuscular mycorrhizal fungi and a plant growth promoting bacterium Pseudomonas putida., Soil. Biol. Biochem., 18, 185-190.
  59. Klyuchnikov A.A. and Kozhevin P.A. (1990)., Dynamics of Pseudomonas fluorescens and Azospirillum brasiliense populations during the formation of the vesicular-arbuscular mycorrhiza., Microbiology (New York), 59(4), 449-452.
  60. Linderman R.G. (1992)., Vesicular-arbuscular mycorrhizae and soil microbial interactions., In: G.J. Bethlenfalvay and R.G.Linderman (eds) Mycorrhizae in Sustainable Agriculture. ASA special Publication, No. 54. American Society of Agronomy, Inc. Crop Science Society of America, Inc, Soil Science Society of America, Inc. Madison, Wisconsin, USA., 45-69.
  61. Tarafdar J.C. and Marshaner H. (1995)., Dual inoculation with Aspergillus fumigatus and Glomus mosse enhances biomass production and nutrient uptake in wheate (Triticum astivam L) supplied with organic phosphorus as Na-phtate., Plant and Soil, 173, 97-102.
  62. Paul E.A. and Clerke F.E. (1989)., Soil Microbiology and Biochemistry., XII + 273 S., 86 Abb., 60 Tab. San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1989.Acedemic press, Inc. New York. ISBN: 0‐12‐546805‐9.\\