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Isolation and Biochemical Characterization of Anti Neoplastic bacteria obtained from Domestic Liquid Waste: A potential Source of Janthinobacterium lividum

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Author Affiliations

  • 1Laboratory of Environment and Biotechnology, Department of Botany, Department of Biochemistry, Patna University-800005, India
  • 2Laboratory of Environment and Biotechnology, Department of Botany, Department of Biochemistry, Patna University-800005, India
  • 3Laboratory of Environment and Biotechnology, Department of Botany, Department of Biochemistry, Patna University-800005, India
  • 4Laboratory of Environment and Biotechnology, Department of Botany, Department of Biochemistry, Patna University-800005, India
  • 5Laboratory of Environment and Biotechnology, Department of Botany, Department of Biochemistry, Patna University-800005, India

Int. Res. J. Environment Sci., Volume 11, Issue (3), Pages 16-26, July,22 (2022)

Abstract

Exploration of the microbial community from the domestic sewage was the principal area of research. The domestic liquid sewage was utilized as a test to contemplate the different variety of the microorganisms present in it. Morphology, growth and pigmentation attributes of the various microorganisms were studied with the assistance of biochemical tests. A screen for antibiosis identified an atypical pale blue-purple producing bacteria, designated as JS-1 and related to Janthinobacterium lividum. Different types of culture media (NA, TYEG, TSA, and LB) were used to isolate and grow the bacteria of interest, JS-1 and it is a gram negativebacilliform capnophillic bacteria. According to the findings, strainJS-1 should indeed be classified as a new strain of Janthinobacterium.

References

  1. Adebayo, F., & Obiekezie, S. (2018)., Microorganisms in Waste Management., Research Journal of Science and Technology, 10(1), 28. https://doi.org/10.5958/2349-2988.2018.00005.0.
  2. Gallo, M., & Ventresca, S. (2016)., The Role of Microorganisms in the Ecosystem., American Society for Microbiology Education Department.
  3. Satyanarayana, T., Johri, B. N., & Prakash, A. (Eds.). (2012)., Microorganisms in environmental management: microbes and environment., Springer Science & Business Media.XXI(1), 1–819.
  4. Ambrožič Avguštin, J., Žgur Bertok, D., Kostanjšek, R., & Avguštin, G.. (2013)., Isolation and characterization of a novel violaceiNegative Resultlike pigment producing psychrotrophic bacterial species Janthinobacterium svalbardensis sp. nov., Antonie Van Leeuwenhoek, 103(4), 763–769. https://doi.org/10.1007/s10482-012-9858-0.
  5. Singh, S. K., Kanth, M. K., Kumar, D., Raj, R., Kashyap, A., Jha, P. K., Anand, A., Puja, K., Kumari, S., Ali, Y., Lokesh, R. S., & Kumar, S. (2017)., Physicochemical and Bacteriological Analysis of Drinking Water Samples from Urban Area of Patna District, Bihar, India., International Journal of Life-Sciences Scientific Research, 3(5), 1355–1359. https://doi.org/10.21276/ijlssr.2017.3.5.15.
  6. Bharucha, E. (2005)., Textbook of environmental studies for undergraduate courses., Universities Press.
  7. Rai, J.P.N., and Rathore, V.S. (1993)., Pollution of Nainital lake water and its Management., Ecology and Pollution of Indian Lakes and Reservoirs, 83-92.
  8. Ramdass, A. C., & Rampersad, S. N. (2021)., Molecular signatures of Janthinobacterium lividum from Trinidad support high potential for crude oil metabolism., BMC microbiology, 21(1), 287. https://doi.org/10.1186/s12866-021-02346-4.
  9. Valdes, N., Soto, P., Cottet, L., Alarcon, P., Gonzalez, A., Castillo, A., Corsini, G., & Tello, M. (2015)., Draft genome sequence of Janthinobacterium lividum strain MTR reveals its mechanism of capnophilic behavior., Standards in genomic sciences, 10, 110. https://doi.org/10.1186/s40793-015-0104-z.
  10. Pantanella, F., Berlutti, F., Passariello, C., Sarli, S., Morea, C., & Schippa, S. (2007)., Violacein and biofilm production in Janthinobacterium lividum., Journal of applied microbiology, 102(4), 992–999. https://doi.org/10.1111/j.1365-2672.2006.03155.x.
  11. Baricz, A., Teban, A., Chiriac, C. M., Szekeres, E., Farkas, A., Nica, M., Dascălu, A., Oprișan, C., Lavin, P., & Coman, C. (2018)., Investigating the potential use of an Antarctic variant of Janthinobacterium lividum for tackling antimicrobial resistance in a One Health approach., Scientific reports, 8(1), 15272. https://doi.org/10.1038/s41598-018-33691-6.
  12. Wilkinson, M. D., Lai, H. E., Freemont, P. S., & Baum, J. (2020)., A Biosynthetic Platform for Antimalarial Drug Discovery., Antimicrobial agents and chemotherapy, 64(5), e02129-19. https://doi.org/10.1128/AAC.02129-19.
  13. Kumar, N., & Shardendu. (2020)., Application of 16 rRNA Gene of V3-V4 Region for Meta Barcoding of Bacterial Community in High Density Population of Eastern India., Bioscience Biotechnology Research Communications, 13(4), 1871–1878. https://doi.org/10.21786/bbrc/13.4/36.
  14. Golterman H.L. (1983)., The Winkler Determination., In: Gnaiger E., Forstner H. (eds) Polarographic Oxygen Sensors. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81863-9_31.
  15. Blenden, D. C., & Goldberg, H. S. (1965)., Silver Impregnation Stain for Leptospira and Flagella., Journal of bacteriology, 89(3), 899–900. https://doi.org/10.1128/jb.89.3.899-900.1965.
  16. Salanitro, J. P., Fairchilds, I. G. & Zgornicki, Y. D. (1974)., Isolation, culture characteristics, and identification of anaerobic bacteria from the chicken cecum., Applied microbiology, 27(4), 678–687. https://doi.org/10.1128/am.27.4.678-687.1974.
  17. Holding, A. J., & Collee, J. G. (1971)., Routine biochemical tests., Methods in Microbiology, 6(A), Academic Press, New York, 2–32.
  18. Stanier, R. Y., Palleroni, N. J. & Doudoroff (1966)., The Aerobic Pseudomonads a Taxonomic Study., Journal of General Microbiology, 43(2), 159–271.
  19. Stolp, H., & Gadkari, D. (1981)., Nonpathogenic members of the genus Pseudomonas., The Prokaryotes, Springer, 1, 719–741. https://link.springer.com/book/10.1007%2F978-94-009-4378-0.
  20. Miller, J. H. (1977)., Formulas and recipes., Experiments in Molecular Genetics, 431–432.
  21. Ostle, A. G., & Holt, J. G. (1982)., Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate., Applied and environmental microbiology, 44(1), 238–241. https://doi.org/10.1128/aem.44.1.238-241.1982.
  22. Hugh, R., & Leifson, E. (1953)., The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria., Journal of bacteriology, 66(1), 24–26. https://doi.org/10.1128/jb.66.1.24-26.1953.
  23. APHA AWWA, W. P. C. F. (1998)., Standard methods for the examination of water and wastewater 20th edition., American Public Health Association, American Water Work Association, Water Environment Federation, Washington, DC.
  24. Lapage, S. P. (1976)., Biochemical tests for identification of medical bacteria., Journal of clinical pathology, 29(10), 958.
  25. Simmons, J. S. (1926)., A Culture Medium for Differentiating Organisms of Typhoid-Colon Aerogenes Groups and for Isolation of Certain Fungi: With Colored Plate., Journal of Infectious Diseases, 39(3), 209–214. https://doi.org/10.1093/infdis/39.3.209.
  26. Barry, A. L., Bernsohn, K. L., Adams, A. P., & Thrupp, L. D. (1970)., Improved 18-hour methyl red test., Applied microbiology, 20(6), 866–870. https://doi.org/10.1128/am.20.6.866-870.1970.
  27. Werkman, C. H. (1930)., An improved technic for the Voges-Proskauer test., Journal of Bacteriology, 20(2), 121-125.
  28. Gillis, M., & De Ley, J. (2006)., The genera Chromobacterium and Janthinobacterium., Prokaryotes, 5, 737-746.
  29. McClung, L. S. (1985)., Bergey
  30. 80.00. International Journal of Systematic and Evolutionary Microbiology, 35(3), 408-408.
  31. Moss, M. O., Ryall, C., & Logan, N. A. (1978)., The Classification and Characterization of Chromobacteria from a Lowland River., Journal of General Microbiology, 105(1), 11–21. https://doi.org/10.1099/00221287-105-1-11.
  32. Ryall, C., & Moss, M. O. (1975)., Selective media for the enumeration of Chromobacterium spp. in soil and water., The Journal of applied bacteriology, 38(1), 53–59. https://doi.org/10.1111/j.1365-2672.1975.tb00500.x.
  33. Friedrich, I., Hollensteiner, J., Schneider, D., Poehlein, A., Hertel, R., & Daniel, R. (2020)., First Complete Genome Sequences of Janthinobacterium lividum EIF1 and EIF2 and their Comparative Genome Analysis., Genome biology and evolution, 12(10), 1782–1788. https://doi.org/10.1093/gbe/evaa148.
  34. Rivero Berti, I., Rodenak-Kladniew, B., Onaindia, C., Adam, C. G., Islan, G. A., Durán, N., & Castro, G. R. (2020)., Assessment of in vitro cytotoxicity of imidazole ionic liquids and inclusion in targeted drug carriers containing violacein., RSC Advances, 10(49), 29336–29346. https://doi.org/10.1039/d0ra05101b.
  35. Choi, S. Y., Lim, S., Cho, G., Kwon, J., Mun, W., Im, H., & Mitchell, R. J. (2020)., Chromobacterium violaceum delivers violacein, a hydrophobic antibiotic, to other microbes in membrane vesicles., Environmental microbiology, 22(2), 705–713. https://doi.org/10.1111/1462-2920.14888.
  36. Park, H., Park, S., Yang, Y. H., & Choi, K. Y. (2021)., Microbial synthesis of violacein pigment and its potential applications., Critical Reviews in Biotechnology, 41(6), 879–901. https://doi.org/10.1080/07388551.2021.1892579.