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Screening of amylase producing bacteria from soil samples of evergreen and deciduous forest

Author Affiliations

  • 1Department of Biotechnology and Research, K.V.M. College of Science and Technology, Kokkothamangalam P. O., Cherthala-688583, Alappuzha District, Kerala, India
  • 2National College of Arts and Science, Kallattumukku, Manacaud, Thiruvananthapuram, Kerala, India
  • 3Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram, Kerala, India
  • 4Department of Biotechnology and Research, K.V.M. College of Science and Technology, Kokkothamangalam P. O., Cherthala-688583, Alappuzha District, Kerala, India

Int. Res. J. Biological Sci., Volume 8, Issue (6), Pages 1-6, June,10 (2019)

Abstract

Amylase is an industrially important enzyme used for the hydrolysis of dietary starch into disaccharides and trisaccharides and ultimately to glucose. Plants, animals and certain microorganisms produce this amylase and are used for various biochemical reactions. The most stable and reliable source of amylase is obtained from microbes when compared to other sources. The purpose of the current study was to isolate and characterize amylase producing bacteria from soil samples collected from the evergreen and deciduous forest of Jawaharlal Nehru Tropical Botanic Garden and Research Institute (JNTBGRI), Palode, Thiruvananthapuram, Kerala, India. The bacterial colonies were isolated through serial dilution and plating techniques. One bacterial colony which showed the maximum zone of clearance in starch agar plates were isolated and cultured separately. The screening was done on the basis of hydrolysis of starch by amylase on agar plate containing 1% starch. The 16S rDNA sequence of the selected strain was isolated and screened using Clustal W programme and a phylogenetic tree was constructed using a Mega 6 software using neighbor joining method. The bacteria were identified as Bacillus sps.

References

  1. Aiyer P.D. (2004)., Effect of C: N ratio on alpha amylase production by Bacillus licheniformis SPT 27., African Journal of Biotechnology, 3(10), 519-522.
  2. Monnet D., Joly C., Dole P. and Bliard C. (2010)., Enhanced mechanical properties of partially beta-amylase trimmed starch for material applications., Carbohydrate Polymers, 80(3), 747-752.
  3. Souza P.M. and Magalhaes P.O. (2010)., Application of Microbial α-Amylase in Industry - A Review., Braz. J. Microbiol., 41(4), 850-861.
  4. Banks W. and Greenwood C.T. (1975)., Starch and its components., Edinberg University press, Edinberg. ISBN 13: 9780470047118.
  5. Dhanya G., Madhavan N.K., Swetha S. and Ashok P. (2009)., Immobilized bacterial alpha amylases for effective hydrolysis of raw starch and soluble starch., Food. Res. Int., 42(4), 436-442.
  6. Calik P. and Ozdamar T.H. (2001)., Carbon sources affect metabolic capacities of Bacillus species for the production of industrial enzymes: theoretical analyses for serine and neutral proteases and alpha-amylase., Biochem. Eng. J., 8(1), 61-81.
  7. Gupta R., Gigras P., Mohapatra H., Goswami V.K. and Chauhan B. (2003)., Microbial α-amylases: a biotechnological perspective., Process Biochem., 38(11), 1599-1616.
  8. Gupta A., Gupta V.K., Modi D.R. and Yadava L.P. (2008)., Production and characterization of α-amylase from Aspergillus niger., Biotechnol., 7(3), 551-556.
  9. Fogarty W.M. and Kelly C.T. (1980)., Microbial Enzymes and Biotechnology., Academic Press Inc, New York, 5, 115-170.
  10. Wind R.D., Buitelaar R.M., Huizing H.J. and Dijkhuizen L. (1994)., Characterization of a new Bacillus stearothermophilus isolate: a highly thermostable α-amylase producing strain., Appl. Micrbiol.Biotechnol., 41(2), 155-162.
  11. Takasaki Y. (1983)., An amylase producing maltotetraose and from maltopentaose from B. circulans., Agric. Biol. Chem., 47(10), 2193-2199.
  12. Brumm P.J., Hebeda R.E. and Teague W.M. (1991)., Purification and characterization of the commercialized, cloned Bacillus megaterium-amylase. Part I: purification and hydrolytic properties., Starch Staerke., 43(8), 315-319.
  13. Takasaki Y. (1985)., An amylase producing maltotriose from B. subtilis., Agric. Biol. Chem., 49(4), 1091-1097.
  14. Murray H.G. and Thompson W.F. (1980)., Rapid Isolation of High Molecular Weight DNA., Nucleic Acids Res., 8(19), 4321-4326.
  15. Lin L.L., Tsau M.R. and Chu W.S. (1994)., General characteristics of thermostable amylopullulanases and amylases from the alkalophilic Bacillus sp. TS-23., Appl. Microbiol. Biotechnol., 42(1), 51-56.
  16. Saxena R.K., Dutt K., Agarwal L. and Nayyar P. (2007)., A highly thermostable and alkaline amylase from a Bacillus sp. PN5., Bioresour. Technol., 98(2), 260-265.
  17. El-Banna T.E., Abd-Aziz A.A., Abou-Dobara M.I. and Ibrahim R.I. (2007)., Production and immobilization of alpha-amylase from Bacillus subtilis., Pak. J.Biol. Sci., 10(12), 2039-2047.
  18. Divakaran D., Chandran A. and Chandran R.P. (2011)., Comparative Study on Production of α-Amylase from Bacillus licheniformis strains., Braz. J. Microbiol., 42(4), 1397-1404.
  19. Pandey A., Nigam P., Soccol C.R., Soccol V.T., Singh D. and Mohan R. (2000)., Advances in microbial amylases., Biotechnol. Appl. Biochem., 31, 135-152.
  20. Smits J.P., Rinzema A., Tramper J., Sonsbeek H.M. and Knol W. (1996)., Solid-state fermentation of wheat bran by Trichoderma reesei QM9414: substrate composition changes, C balance, enzyme production, growth and kinetics., Appl. Microbiol. Biotechnol., 46, 489-496.