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The effects of heat and molotov incendiary device fluids on DNA analysis

Author Affiliations

  • 1Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
  • 2Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
  • 3Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
  • 4Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
  • 5Institute of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
  • 6Faculty of Pharmacy, Mersin University, Mersin, Turkey
  • 7Faculty of Health Sciences, Istanbul Yeni Yuzyil University, Istanbul, Turkey
  • 8Department of Forensic Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey
  • 9Faculty of Health Sciences, Istanbul Yeni Yuzyil University, Istanbul, Turkey

Res. J. Forensic Sci., Volume 7, Issue (2), Pages 8-14, July,29 (2019)

Abstract

Using the findings from terror attacks such as hand-made incendiary devices, the chemical constituency of the explosive can be elucidated to identify the terrorist group responsible for the attack. It is also possible to clarify the incident by analyzing the DNA from the fingerprint. The purpose of this study is to obtain DNA from the fingerprint found on the explosive at the crime scene and to analyze the thermal effect of the DNA on profiling. Fingerprint samples were put on glass, paper and metal sheet surfaces and were individually exposed to diffrent heat conditions for 3 minutes. A total of 108 samples were subjected to DNA analysis using GlobalFiler® PCR Amplification Kit and were typed using the ABI 3130 electrophoresis device. From the samples, complete DNA profile was obtained at 50oC and 90oC on the paper surface and at 50oC on the glass and metal surfaces. On all surfaces and at all temperatures, the gender-identifying locus, amelogenin, was able to be observed. This study, which is conducted for the first time in Turkey, may significantly contribute to the identification of attackers and clarification of the events particularly in terror acts.

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