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Detection of mecA Gene in Methicillin resistance Staphylococcus aureus (MRSA) from rural chickens, slaughter environment and personnel in Maiduguri, Borno State, Nigeria

Author Affiliations

  • 1Department of Veterinary Medicine, University of Maiduguri, Nigeria
  • 2Department of Veterinary Medicine, University of Maiduguri, Nigeria
  • 3Department of Veterinary Microbiology, University of Maiduguri, Nigeria
  • 4Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Nigeria
  • 5Department of Veterinary Medicine, University of Maiduguri, Nigeria
  • 6Department of Animal Health and Production, College of Agriculture Gujba, Yobe State, Nigeria

Res. J. Animal, Veterinary and Fishery Sci., Volume 6, Issue (2), Pages 1-7, March,24 (2018)


Cross sectional study was carried out from Monday and Gamboru markets in Maiduguri, Nigeria to detect the presence of methicillin-resistant Staphylococcus aureus (MRSA) and determine the interplay between rural chickens, slaughter environment and personnel in the spread of MRSA. Samples were taken from skin of rural chickens, dressing tables, containers, knives and from the skin and nostrils of slaughter personnel. A total of 135 presumptive S. aureus isolates were analyzed and subjected to ORSAB test to identify MRSA using standard techniques. The test detected 57 (42.2%) MRSA isolates out of which 25 were randomly selected and subjected to PCR to detect S. aureus specific sequence gene and mecA gene. The isolates showed evidence of expression of S. aureus specific sequence gene with 107 bp targeted band whilst only 1 isolate was positive for mecA gene with a targeted band of 532 bp. It is therefore evident from this result that mecA gene is present in MRSA isolate from rural chicken which means transmission between human and animals is possible. Awareness programs should be implemented to educate the slaughter personnel and general public on its veterinary and public health importance. Therefore, these emphasize the need for further research to be carried out on the phylogenesis of MRSA in poultry and other livestock in Nigeria.


  1. Eshetu Y., Mulualem E., Ibrahim H., Berhanu A. and Aberra K. (2001)., Study of gastro-intestinal helminths of scavenging chickens in four rural districts of Amhara region, Ethiopia., Revue Scientifique Et Technique-Office International Des Epizooties, 20(3), 791-793.
  2. Osei S.A. and Dei H.K. (1998)., Poultry production on small scale farms in developing countries., Proceedings of the 8th World Conference on Animal Production, Seoul, South Korea. Symposium Series 2, 376-384.
  3. Alders R. and Spradbrow P.B. (2000)., Newcastle disease in village chickens: a field manual., Maputo, Mozambique, 46.
  4. Sakoulas G. and Moellering R.C. (2008)., Increasing antibiotic resistance among methicillin-resistant Staphylococcus aureus strains., Journal of Clinical and Infectious Diseases, 46(5), 360-367. https://doi.org/10.1086 /533592
  5. CDC (2009)., Healthcare-Associated Methicillin Resistant Staphylococcus aureus (HA-MRSA)., Retrieved April 24, 2009, from www.cdc.gov/ncidod/dhqp/ar_MRSA.html
  6. Sexton T., Clarke P., O, Environmental reservoirs of methicillin-resistant Staphylococcus aureus in isolation rooms: correlation with patient isolates and implications for hospital hygiene., Journal of Hospital Infection, 62(2), 187-194.
  7. Kwon N.H., Park K.T., Moon J.S., Jung W.K., Kim S.H., Kim J.M. and Park Y.H. (2005)., Staphylococcal cassette chromosome mec (SCC mec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCC mec subtype IVg isolated from bovine milk in Korea., Journal of Antimicrobial Chemotherapy, 56(4), 624-632.
  8. Nworie A., Azi S.O., Ibiam G.A., Egwu I.H., Odoh I., Oti-Wilberforce R.O. and Obi I.A. (2013)., Nasal carriage of methicillin resistant Staphylococcus aureus amongst meat sellers in Abakaliki Metropolis, Ebonyi State, Nigeria., Microbiology Research International, 1(3), 48-53.
  9. Armand-Lefevre L., Ruimy R. and Andremont A. (2005)., Clonal comparison of Staphylococcus aureus isolates from healthy pig farmers, human controls, and pigs., Emerging infectious diseases, 11(5), 711-114.
  10. Voss A., Loeffen F., Bakker J., Klaassen C. and Wulf M. (2005)., Methicillin resistant Staphylococcus aureus in pig farming., Emerging Infectious Diseases, 11(12), 1965-1966.
  11. Price L.B., Stegger M., Hasman H., Aziz M., Larsen J., Andersen P.S., Pearson T., Andrew E.W., Jeffrey T.F., James S., John G., Elizabeth D., Cindy M.L., Burkhard S., Irena Z., Battisti A., Franco A., Jacek Z. M., Stefan S., Patrick B., Eric J., Pomba C., Porrero M.C., Raymond R., Tara C.S., Robinson D.A., Weese J.S., Carmen S.A., Fangyou Yu, Frederic L., Paul K., Robert S. and Aarestrup F.M. (2012)., Staphylococcus aureus CC398: host adaptation and emergence of methicillin resistance in livestock., mBio, 3, e00305-e00311. DOI: 10.1128/mBio.00305-11
  12. Catry B., Van Duijkeren E., Pomba M.C., Greko C., Moreno M.A., Pyörälä S. and Törneke K. (2010)., Scientific Advisory Group on Antimicrobials (SAGAM) Reflection paper on MRSA in food-producing and companion animals: epidemiology and control options for human and animal health., Epidemiol Infect, 138(5), 626-644.
  13. Van Cleef B.A.G.L., Broens E.M., Voss A., Huijsdens X.W., Züchner L., Van Benthem B.H.B. and Van De Giessen A.W. (2010)., High prevalence of nasal MRSA carriage in slaughterhouse workers in contact with live pigs in The Netherlands., Epidemiology & Infection, 138(5), 756-763.
  14. Graveland H., Wagenaar J.A., Heesterbeek H., Mevius D., Van Duijkeren E. and Heederik D. (2010)., Methicillin resistant Staphylococcus aureus ST398 in veal calf farming: human MRSA carriage related with animal antimicrobial usage and farm hygiene., PloS one, 5(6), e10990.
  15. Mulders M.N., Haenen A.P.J., Geenen P.L., Vesseur P.C., Poldervaart E.S., Bosch T. and Mevius D. (2010)., Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The Netherlands., Epidemiology & Infection, 138(5), 743-755.
  16. Van Belkum A., Melles D.C., Peeters J.K., van Leeuwen W.B., van Duijkeren E., Huijsdens X.W. and Verbrugh H.A. (2008)., Dutch Working Party on Surveillance and Research of MRSASOM. Methicillin-resistant andsusceptible Staphylococcus aureus sequence type 398 in pigs and humans., Emerging Infect. Dis, 14, 479-483.
  17. Bauer A.W., Kirby W.M., Sherris J.C. and Turck M. (1966)., Antibiotic susceptibility testing by a standardized single method., American journal of Clinical Pathology, 45(4), 493-496.
  18. Martineau F.P., Roy P.M. and Bergeron M.G. (1998)., Species-specific and ubiquitous- DNA based assay for rapid identification of staphylococcus aureus., Clinical Microbiology, 36(3), 618-623.
  19. Strommenger B., Kettlitz C., Werner G. and Witte W. (2003)., Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus., Journal of Clinical Microbiology, 41(9), 4089-4094.
  20. García-Álvarez L., Holden M.T., Lindsay H., Webb C.R., Brown D.F., Curran M.D. and Parkhill J. (2011)., Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study., The Lancet infectious diseases, 11(8), 595-603.
  21. Stefania S., Chung D.R., Lindsay J.A., Friedrich A.W., Kearns A.M., Westh H. and MacKenzie F.M. (2012)., Meticillin-resistant Staphylococcus aureus (MRSA): global epidemiology and harmonisation of typing methods International., Journal of Antimicrobial Agents, 39(4), 273-282.
  22. Fitzgerald J.R. (2012)., Livestock-associated Staphylococcus aureus: origin, evolution and public health threat., Trends in microbiology, 20(4), 192-198.