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Bioefficacy and Progeny Production of native new-to-science species of entomopathogenic nematodes, Steinernema dharanaii (TFRIEPN-15) against forest insect pest, Albizia defoliator, Spirama retorta Cramer (Lepidoptera: Noctuidae)

Author Affiliations

  • 1Northern Regional Centre, Zoological Survey of India, 218, Kaulagarh Road, Deharadun-248 195, Uttarakhand, India
  • 2Forest Entomology Division, Tropical Forest Research Institute, P. O. RFRC, Mandla Road, Jabalpur- 482021, Madhya Pradesh, India and Institute of Forest Productivity (IFP) Gumla, National Highway-23, Lalgutwa, Ranchi – 835303, Jharkhand, India

Res. J. Agriculture & Forestry Sci., Volume 7, Issue (4), Pages 10-16, October,8 (2019)

Abstract

The laboratory study examined bioefficacy and progeny production of locally isolated new –to science species of entomopathogenic nematode (EPNTFRI-15), Steinernema dharanaii against forest insect pest, Albizia defoliator, Spirama reortra Cram. (Lepidoptera: Noctuidae) in the laboratory. The larvae were exposed to ten dosages viz., 3, 5, 10, 15, 20, 30, 40, 50, 100 and 200IJs larva-1 plate. This EPN species for different time periods and they were found to be susceptible to all the doses tested in filter paper bioassay. However, the susceptibility and progeny production of larvae to nematode infection varied according to the dosages of IJs and their exposure periods. The doses of 3IJs Larva-1 exhibited negligible but statistically significant mortality (17.14%) over untreated control (P<0.05).There were significantly superior (P<0.05) mortalities 74.29%, 100.0% and recorded at 50, 100 and 200IJs Larva-1. The progeny production data from the larvae exposed to different doses of IJs indicated progeny production to be proportional to the IJ doses up to 100IJs Larva-1 at which it was significantly higher 37,400 IJs (P<0.05), which decreased with increase in IJ doses. However, progeny production at the highest dose of 200 IJs Larva-1 (18,180IJs) was still significantly superior over IJs obtained at the lowest dose (P<0.05). The probit analysis performed, indicated 7.07 (UL 11.47 and LL 4.37IJs Larva-1) and 34.67IJs Larva-1 (UL 51.32 and LL 23.42IJs Larva-1) were required to cause, respectively 50 and 90.0% mortality in albizia defoliator larvae in laboratory (P<0.05) . At the same time 34.51 (UL 80.60 and LL 14.42 hrs) and 131.82 hrs (UL 218.98 and LL 79.35hrs) were required for causing 50 and 90.0% mortality, respectively. This locally isolated new-to-science EPN species, Steinernmea dharanaii thus have potential scope for the management of major insect pests of forestry and agricultural importance in India.

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