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Eficacy of Gloriosa Superba L. On Plant Pathogenic Fungi

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Author Affiliations

  • 1Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, Jharkhand, INDIA
  • 2Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, Jharkhand, INDIA
  • 3Department of Veterinary Pathology, Bihar Veterinary College, Patna-800014, Bihar, INDIA
  • 4Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, Jharkhand, INDIA
  • 5Laboratory of Plant Physiology and Biotechnology, University Department of Botany, Ranchi University, Ranchi, Jharkhand, INDIA

Res. J. Agriculture & Forestry Sci., Volume 3, Issue (3), Pages 1-6, March,8 (2015)

Abstract

Gloriosa superba is a endangered toxic plant belong the family Colchicaceae (Liliaceae) commonly known as kalihari (Hindi), glory lily (English) containing high levels of colchicines and gloriocine both are toxic alkaloid. Traditionally it is used for the treatment of various diseases. The aim of present research screening of phytochemical profile and evaluate the Antifungal activity of Gloriosa superba against four plant pathogenic fungal spp. namely Fusarium oxysporum, Alternaria species, Sclerotium rolfsii and Rhizoctonia solani. These selected fungi causes a wide range of commercially significant plant disease; some time also in animals and human. To determine the antifungal activity, food poisoning method was employed. Different concentration of methanoic extract (6.25, 12.5, 25, 37.5, 50, 62.5 µg/ml) of Gloriosa superba were tested against fungal strain and the phytochemical screening were perform by standard harbone method. The methanol extract of Gloriosa superba L. showed very good antifungal activity against selected plant pathogen fungi. The excellent inhibitory activity was observed against Rhizoctonia solanii (99.33%) and Alternaria sp. (98.13%) followed by Sclerotium rolfsii (84.26%) and Fusarium oxysporum (63.23%) at the concentration of 62.5 µg/ml. The results of the phytochemical screening revealed the presence of alkaloids, steroid, carbohydrates, tannins, phenolic compounds, terpenoid, cardiac glycoside, flavonoids and absence of phlobotannin, saponin, anthraquinone. It may be due to presence of phytochemicals like colchicines (alkaloid) plant show antifungal activity. The excellent inhibitory results conclude that the crude plant extact can be used as alternative of biopesticide and some antifungal drugs.

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  24. Cleeland R.S. and Squires E., Evaluation of new antimicrobials in vitro and in experimental animal infections, In: Lorian VMD (ed), Antibiotics in Laboratory Medicine, Baltimore : Williams and Wilins, 739-88 (1991)
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  26. Ceolho D., Souza G., Haas A.P.S., Von Poser G.L., Schapova E.E.S. and Elisabetsky E., Ethnopharmacological studies of antimicrobial remedies in the south of Brazil, J Ethnopharmacol, 90, 135-43 (2004)
  27. Sanders F.T., The Role of the EPA in the Regulation of Antimicrobial Pesticides in the United States, Pesticide Outlook, 14 (2), 251-255 (2003)
  28. Haroon K., Murad A.K., Tahira M. and Muhammad I.C., Antimicrobial activities of Gloriosa superba Linn (Colchicaceae) extracts, Journal of enzyme inhibition and medicinal chemistry, (2008)
  29. Jain P., Sharma H.P., Pathak C. and Anita, Antimicrobial Activity and Phytochemical Analysis of Plumbago zeylanica L. (An Anti-Cancer Plant), Journal of International Academic Research For Multidisciplinary, 2(4), 695-706 (2014)
  30. Jain P., Kumari S.S., Sharma H.P. and Basri F., Phytochemicals Screening and Antifungal Activity of Semecarpus anacardium L. (An Anti-Cancer Plant), International Journal of Pharmaceutical Sciences and Research, 5(5), 1884-1891 (2014)