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Evaluating genetic diversity and relationships among eastern Indian Moringa oleifera Lam. genotypes at molecular level

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Author Affiliations

  • 1ICFRE-Eco-Rehabilitation Centre, 3/1 Lajpatrai Rod, Prayagraj-211002, India
  • 2Jharkhand State Livelihood Promotion Society, Ranchi, India

Res. J. Agriculture & Forestry Sci., Volume 11, Issue (3), Pages 15-28, November,8 (2023)

Abstract

Genetic diversity and relationships among 24 genotypes of Moringa oleifera collected from wide geographical range consisting of 11 agroclimatic zones in eastern India was studied employing Random Amplified Polymorphic DNA (RAPD) and microsatellite markers. Both the markers appeared as highly informative for assessment of intra-specific genetic diversity among populations of M. oleifera from different geographical areas. A total of 137 fragments ranging in size from 0.4–3.3 kb with 87.63% polymorphic loci were produced from the amplification profiles of total genomic DNA from the genotypes with 25 RAPD primers. The extent of genetic diversity across the genotypes was also evaluated employing 10 microsatellite markers dispersed across the genome. Total 87 alleles were identified with the help of 9 markers. The number of allele per locus varied from 5 to 15, with a mean of 9.66 allele per locus. 100 % polymorphism was exhibited by the makers. Polymorphism in the present study as well as Nei’s mean estimates of diversity- H can be considered sufficiently high. Assemblage of genotypes irrespective of geographical locations in the cluster analysis through both the marker systems indicates high within population genetic variation possibly due to seed source variability, mutations and/or breeding systems. The study reveals that there exists sufficient genetic variation among genotypes from different areas of eastern India, despite the fact that the species has acquired status of a predominantly clonal propagated species in the region. As eastern India is the native range, genotypes collected from the region should be considered while selecting materials for improvement of cultivated individuals and conservation of M. oleifera genetic resources.

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